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Evaluation of the NanoCHIP® Infection Control Panel test for direct detection and screening of methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria and vancomycin-resistant Enterococcus (VRE)

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Abstract

Purpose

Rapid detection of infection control targets is needed and several bacterial target assays are commercially available. Detection of patients colonized with Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae (KPC-CRE), methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) comprises an essential part of infection control programs. This study evaluated the performance and feasibility of a novel molecular-based diagnostic screening test, the NanoCHIP® Infection Control Panel (ICP) assay (Savyon Diagnostics, Israel), which enables simultaneous detection of KPC-CRE, MRSA and VRE directly from swab samples and compares its sensitivity and specificity to culture.

Methods

Prospective direct swab analysis of 338 (70 CRE, 198 MRSA and 70 VRE) screening swab samples.

Results

Including all targets and all valid samples, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of the NanoCHIP® ICP assay were 91.1, 99.5, 99.1 and 94.9 %, respectively.

Conclusions

As far as we know, this is the first report regarding a single molecular-based system that detects all three targets (CRE-KPC, MRSA and VRE) simultaneously, directly from swab samples, using the same reaction and platform. Overall, the assay was easy to perform, enabling medium- to high-throughput screening. Same day results enable efficient infection control interventions, such as carrier isolation.

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Acknowledgments

This work was supported in part by a grant from Savyon Diagnostics.

Conflict of interest

Dr. Paitan has served as a consultant to Savyon Diagnostics since June 2011. The other authors have no potential conflicts of interest to declare.

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Author notes

    Authors and Affiliations

    1. Clinical Microbiology Laboratory, Meir Medical Center, 59 Tschernihovsky St., 44281, Kfar Saba, Israel

      Judith Weiss, Haia Arielly, Nirit Ganor & Yossi Paitan

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    1. Judith Weiss
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    2. Haia Arielly
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    3. Nirit Ganor
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    4. Yossi Paitan
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    Corresponding author

    Correspondence to Yossi Paitan.

    Additional information

    J. Weiss, H. Arielly and N. Ganor contributed equally to the routine microbiology diagnostics.

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    Weiss, J., Arielly, H., Ganor, N. et al. Evaluation of the NanoCHIP® Infection Control Panel test for direct detection and screening of methicillin-resistant Staphylococcus aureus (MRSA), Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria and vancomycin-resistant Enterococcus (VRE). Infection 43, 331–338 (2015). https://doi.org/10.1007/s15010-015-0754-1

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