Abstract
BACKGROUND:
Despite the many advantages of recombinant subunit vaccines, they have critical weaknesses that include a low efficacy for promoting cellular and humoral immune responses against antigens because of their poor immunogenicity, and a rapidly cleared properties as a result of proteolytic enzymes in the body. To circumvent these problems, we developed mannan-decorated inulin acetate microparticles (M-IA MPs) that functioned as carriers and adjuvants for immunization with the recombinant foot-and-mouth disease multi-epitope subunit vaccine (M5BT).
METHODS:
The M5BT-loaded M-IA MPs were obtained by a double-emulsion solvent-evaporation method. Their properties including morphology, size and release ability were determined by field emission scanning electron microscope, dynamic light-scattering spectrophotometer and spectrophotometer. To assess the immunization efficacy of the MPs, mice were immunized with MPs and their sera were analyzed by ELISA.
RESULTS:
The M-IA MPs obtained by a double-emulsion solvent-evaporation method were spherical and approximately 2–3 µm, and M5BT was encapsulated in the M-IA MPs. The M5BT-loaded M-IA MPs showed higher antigen-specific IgG, IgG1, IgG2a and anti-FMDV antibodies than the M5BT-loaded IA MPs and the Freund’s adjuvant as a control.
CONCLUSION:
The M-IA MPs showed a powerful and multifunctional polymeric system that combined two toll-like receptor agonists compared to the conventional adjuvant.
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Acknowledgements
This works was founded by the Ministry of Science, ICT and Future Planning (Project No. 2016R1D1A1B03933491). The first authors were supported by Brain Korea 21 Plus program.
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Mouse in vivo experiment were carried out following the policy and regulations for the care and use of laboratory animal (Laboratory animal center, Seoul National University, Korea). All protocols were reviewed and approved by the Animal Care and Use Committee at Seoul National University (SNU-141201-1).
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Yoon, SY., Kang, SK., Lee, HB. et al. Enhanced Efficacy of Immunization with a Foot-and-Mouth Disease Multi-Epitope Subunit Vaccine Using Mannan-Decorated Inulin Microparticles. Tissue Eng Regen Med 17, 33–44 (2020). https://doi.org/10.1007/s13770-019-00228-5
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DOI: https://doi.org/10.1007/s13770-019-00228-5