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Optimization of ultrasonic-assisted extraction of flavonoid from Portulaca oleracea L. by response surface methodology and chemical composition analysis

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Abstract

Optimization of ultrasonic extraction of Portulaca oleracea L. flavonoids (POF) was investigated using single-factor experimentation combined with response surface methodology. The optimal conditions for the highest yield (16.25 mg RE/g DW) of POF was 39.01% ethanol, 55.25°C extraction temperature, 15 min extraction time, and 23.92 (v/m) liquid-to-solid ratio. The crude extract of POF was purified on the polyamide resin. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a yellow tetrazole assays of the flavonoids were evaluated, which suggested the concentrations of the flavonoids (0–1 mg/mL) and quercetin (0–1 mg/mL). Quercetin was identified in the extract by comparing relative retention time of the reference standard.

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Authors and Affiliations

  1. School of Life Science, Jiangxi Science & Technology Normal University, Nanchang, Jiangxi, 330013, China

    Chenxi Wang, Yuping Li, Lihua Yao, Guangjie Wu, Jun Chang, Chengchuang Shu & Meiqin Chen

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  1. Chenxi Wang
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  2. Yuping Li
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  3. Lihua Yao
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  4. Guangjie Wu
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  5. Jun Chang
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  6. Chengchuang Shu
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  7. Meiqin Chen
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Correspondence to Yuping Li.

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Wang, C., Li, Y., Yao, L. et al. Optimization of ultrasonic-assisted extraction of flavonoid from Portulaca oleracea L. by response surface methodology and chemical composition analysis. J Korean Soc Appl Biol Chem 57, 647–653 (2014). https://doi.org/10.1007/s13765-014-4058-4

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  • DOI: https://doi.org/10.1007/s13765-014-4058-4

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