Abstract
Stoneflies (Plecoptera) are aquatic insects widely used in ecological and monitoring studies as indicators of environmental quality due to their high sensitivity to pollution. Among Brazilian Plecoptera, Perlidae is the most diverse family, with four genera (Anacroneuria Klapálek 1909, Enderleina Jewett 1960, Kempnyia Klapálek 1914, and Macrogynoplax Enderlein 1909) and about 150 species. Herein, based on data from the barcode region of the mitochondrial cytochrome c oxidase subunit I (COI) gene and morphological characters, we studied specimens of Enderleina and Macrogynoplax, mainly from the Brazilian Savanna. Our alignment with a total length of 608 base pairs included 23 COI sequences, of which 10 are new and 13 were obtained from GenBank. The sequences and congruence of the obtained species clusters were analyzed using neighbor joining. As a result, we describe a new species of Enderleina, the first of this genus in the Brazilian Savanna, and update the geographic distribution of some Macrogynoplax species. A new record was included for Macrogynoplax veneranda Froehlich 1984 in São Paulo state and the first record of M. yupanqui Stark 1996 in Brazil was also included. First records were made for Macrogynoplax matogrossensis Bispo and Neves 2005 and a Macrogynoplax specimen in Goiás and Bahia states, respectively. Consequently, we expanded the distribution of the genus in the Brazilian Savanna and Atlantic Forest. In addition, we provide observations on the morphological differences found among these species, discuss about the distribution of the genera, present molecular data discussing their contributions to the knowledge of the genus, and comment on the recommendations for future research considering a scenario of integrative approaches. Lastly, we present a key to identify the adult males of Enderleina.
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Acknowledgements
The authors would like to thank all collectors and their team for sampling the material and the federal funding agencies for financially supporting this study (CNPq and FAPESP). The authors thank Dr. Neusa Hamada, for sending us some specimens of Enderleina froehlichi, which were essential for the development of the current study. The authors are especially grateful to Dr. Lourivaldo Amancio de Castro, for his help in showing the Enderleina collection locality in Nova Xavantina, Mato Grosso state, Brazil.
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Zoobank urn:lsid:zoobank.org:pub:35A15944-1C80-47EF-A7F6-BB6D37E569EB.
Funding
Research Foundation of São Paulo (FAPESP, 2019/22833–0 and BIOTA 2021/05986–8) and National Council for Scientific and Technological Development (CNPq, PROTAX 441119/2020–4). LHA had postdoctoral fellowships of CNPq (PROTAX 153038/2022–5) and of the São Paulo State University (UNESP). PNT had Master fellowship of FAPESP (22/15689–3) and VML PhD fellowship of CNPq (142299/2020–0). PCB had Research Productivity Fellowship of CNPq (306400/2022).
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All authors contributed to the study conception and design. All authors performed species sampling, material preparation, and analysis. L. H. A. and P. C. B. described the new species. L. H. A. wrote the first draft of the manuscript and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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de Almeida, L.H., Taniguti, P.N., Lopez, V.M. et al. Taxonomic Contributions on Macrogynoplax Enderlein and Enderleina Jewett (Plecoptera: Perlidae) from Brazil, Including a New Species Supported by Molecular and Morphological Data. Neotrop Entomol 52, 1119–1128 (2023). https://doi.org/10.1007/s13744-023-01084-8
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DOI: https://doi.org/10.1007/s13744-023-01084-8