Skip to main content

Advertisement

SpringerLink
Log in

Double injection–single detector flow injection spectrophotometric method for simultaneous determination of ascorbic acid and paracetamol in pharmaceutical formulations

  • Original Paper
  • Published:
Journal of the Iranian Chemical Society Aims and scope Submit manuscript

Abstract

A simple and low-cost flow injection spectrophotometric method was proposed for the simultaneous determination of ascorbic acid (AA) and paracetamol (PCM) in pharmaceutical formulations. The present method is based on the reduction of Fe(III) to Fe(II) with AA and PCM in a closed flow system which subsequently combines with the 1,10-phenanthroline to form an orange-coloured complex that is measured at 510 nm. The increase in the absorbance was proportional to the concentration of AA in the range of 0.1–12 µg/ml with a correlation coefficient of 0.9997 and detection limit of 0.31 µg/ml, and to the concentration of PCM in the range of 0.4–24 µg/ml with a correlation coefficient of 0.9999 and detection limit of 0.39 µg/ml. The precision for ten serial estimations of 2 and 7 µg/ml of AA exhibited a relative standard deviation of 3.33% and 2.47% and of 6 and 15 µg/ml of PCM with a relative standard deviation of 3.008% and 2.23%, respectively. The present FI method permits determination of 9 samples per hour with no interference from the common excipients. The developed method was applied to different tablets containing AA and PCM with the recovery of AA from four spiked samples ranged from 95.31 to 104.73%, and of PCM ranged from 95.03 to 104.93%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12

Similar content being viewed by others

References

  1. A. Selimović, M. Salkić, A. Selimović, Int. J. Basic Appl. Sci. 11, 106 (2011)

    Google Scholar 

  2. H. Lullman, H. Lutz, M. Klaus, B. Detlef, Color Atlas of Pharmacology, 3rd edn. (Thieme, Stuttgard, 2005), p. 198

    Google Scholar 

  3. H.R. Zare, B. Moradiyan, Z. Shekari, A. Benvidi, Measurement 90, 510 (2016)

    Google Scholar 

  4. S.P. Arya, M. Mahajan, P. Jain, Indian J. Chem. 38, 1303 (1999)

    Google Scholar 

  5. S.H. Davies, S.J. Masten, Anal. Chim. Acta 248, 225 (1991)

    CAS  Google Scholar 

  6. X. Gu, C. Chen, T. Zhou, Fresenius J. Anal. Chem. 355, 94 (1996)

    CAS  Google Scholar 

  7. K. Güçlü, K. Sözgen, E. Tütem, M. Özyürek, R. Apak, Talanta 65, 1226 (2005)

    PubMed  Google Scholar 

  8. S.S. Hassan, M.A. El Fattah, M. Zaki, Fresenius’ Zeitschrift für analytische Chemie 277, 369 (1975)

    CAS  Google Scholar 

  9. H.-H. Shieh, T.R. Sweet, Anal. Biochem. 96, 1 (1979)

    CAS  PubMed  Google Scholar 

  10. A. Jain, A. Chaurasia, K.K. Verma, Talanta 42, 779 (1995)

    CAS  PubMed  Google Scholar 

  11. A. Molina-Dıaz, I. Ortega-Carmona, M. Pascual-Reguera, Talanta 47, 531 (1998)

    PubMed  Google Scholar 

  12. A.V. Pereira, O. Fatibello-Filho, Talanta 47, 11 (1998)

    CAS  PubMed  Google Scholar 

  13. D.G. Themelis, P.D. Tzanavaras, F.S. Kika, Talanta 55, 127 (2001)

    CAS  PubMed  Google Scholar 

  14. M. Yebra, R. Cespon, A. Moreno-Cid, Anal. Chim. Acta 448, 157 (2001)

    CAS  Google Scholar 

  15. M. Noroozifar, M. Khorasani-Motlagh, Talanta 61, 173 (2003)

    CAS  PubMed  Google Scholar 

  16. M.R. Rama, A.R. Medina, A.M. Dı́az, Microchem. J. 78, 157 (2004)

    Google Scholar 

  17. A.B. Vishnikin, T.Y. Svinarenko, H. Sklenářová, P. Solich, Y.R. Bazel, V. Andruch, Talanta 80, 1838 (2010)

    CAS  PubMed  Google Scholar 

  18. L. Kukoc-Modun, M. Biocic, N. Radić, Talanta 96, 174 (2012)

    CAS  PubMed  Google Scholar 

  19. R. Hassan, A. Faizullah, J. Iran. Chem. Soc. 8, 662 (2011)

    CAS  Google Scholar 

  20. G.G. Rao, G.S. Sastry, Anal. Chim. Acta 56, 325 (1971)

    CAS  PubMed  Google Scholar 

  21. M. Barakat, S. Shehab, N. Darwish, E. El-Zoheiry, Anal. Biochem. 53, 245 (1973)

    CAS  PubMed  Google Scholar 

  22. D.E. Hughes, J. Pharm. Sci. 72, 126 (1983)

    CAS  PubMed  Google Scholar 

  23. K.G. Kumar, P. Indrasenan, Talanta 37, 269 (1990)

    CAS  PubMed  Google Scholar 

  24. S.M. Sultan, Talanta 40, 593 (1993)

    CAS  PubMed  Google Scholar 

  25. B. Vahid, J. Chem. Soc. Pak.34, 1510–1512 (2012)

    CAS  Google Scholar 

  26. M. Koupparis, T. Hadjiioannou, Anal. Chim. Acta 94, 367 (1977)

    CAS  Google Scholar 

  27. J.C.B. Fernandes, L. Rover Jr., L.T. Kubota, G.D. Oliveira Neto, J. Braz. Chem. Soc. 11, 182 (2000)

    CAS  Google Scholar 

  28. R. Thangamuthu, S.S. Kumar, K.C. Pillai, Sens. Actuators B Chem. 120, 745 (2007)

    CAS  Google Scholar 

  29. M. Motshakeri, J. Travas-Sejdic, A.R.J. Phillips, P.A. Kilmartin, Electrochim. Acta 265, 184 (2018)

    CAS  Google Scholar 

  30. M. Doulache, M. Trari, B. Saidat, J. Chem. Sci. 130, 110 (2018)

    Google Scholar 

  31. A.A. Hathoot, K.M. Hassan, W.A. Essa, M. Abdel-Azzem, J. Iran. Chem. Soc. 14, 1789 (2017)

    CAS  Google Scholar 

  32. K.M. Hassan, J. Iran. Chem. Soc. 15, 1007–1014 (2018)

    CAS  Google Scholar 

  33. T. Maki, N. Soh, K. Nakano, T. Imato, Talanta 85, 1730 (2011)

    CAS  PubMed  Google Scholar 

  34. Y. Matsuoka, M. Yamato, T. Yamasaki, F. Mito, K.-I. Yamada, Free Radic. Biol. Med. 53, 2112 (2012)

    CAS  PubMed  Google Scholar 

  35. G. Xue, Z. Yue, Z. Bing, T. Yiwei, L. Xiuying, L. Jianrong, J. Lumin. 180, 146 (2016)

    CAS  Google Scholar 

  36. X. Li, A.A. Franke, J. Chromatogr. B 877, 853 (2009)

    CAS  Google Scholar 

  37. M. Ciulu, S. Solinas, I. Floris, A. Panzanelli, M.I. Pilo, P.C. Piu, N. Spano, G. Sanna, Talanta 83, 924 (2011)

    CAS  PubMed  Google Scholar 

  38. R. Kanďár, P. Drábková, R. Hampl, J. Chromatogr. B 879, 2834 (2011)

    Google Scholar 

  39. R. Ferin, M.L. Pavão, J. Baptista, Clin. Biochem. 46, 665 (2013)

    CAS  PubMed  Google Scholar 

  40. R. Zuo, S. Zhou, Y. Zuo, Y. Deng, Food Chem. 182, 242 (2015)

    CAS  PubMed  Google Scholar 

  41. T.Z. Attia, Spectrochim. Acta Part A Mol. Biomol. Spectrosc. 169, 82 (2016)

    CAS  Google Scholar 

  42. F. Turak, R. GŘzel, E. Dinš, J. Food Drug Anal. 25, 285 (2017)

    CAS  PubMed  Google Scholar 

  43. J.T. Afshari, T.-Z. Liu, Anal. Chim. Acta 443, 165 (2001)

    CAS  Google Scholar 

  44. Z.A. ALOthman, M.A. Abdalla, Arab. J. Chem. 4, 239 (2011)

    CAS  Google Scholar 

  45. H. Filik, M. Hayvali, E. Kilic, Anal. Chim. Acta 535, 177 (2005)

    CAS  Google Scholar 

  46. F.A. Mohamed, M.A. AbdAllah, S.M. Shammat, Talanta 44, 61 (1997)

    CAS  PubMed  Google Scholar 

  47. P. Nagaraja, K.S. Murthy, K. Rangappa, J. Pharm. Biomed. Anal. 17, 501 (1998)

    CAS  PubMed  Google Scholar 

  48. Z. Bouhsain, S. Garrigues, A. Morales-Rubio, M. de la Guardia, Anal. Chim. Acta 330, 59 (1996)

    CAS  Google Scholar 

  49. A.R. Medina, M.F. de Córdova, A.M. Dıaz, Anal. Chim. Acta 394, 149 (1999)

    Google Scholar 

  50. A. Criado, S. Cárdenas, M. Gallego, M. Valcárcel, Talanta 53, 417 (2000)

    CAS  PubMed  Google Scholar 

  51. M. Knochen, J. Giglio, B.F. Reis, J. Pharm. Biomed. Anal. 33, 191 (2003)

    CAS  PubMed  Google Scholar 

  52. A.F. Lavorante, C.K. Pires, B.F. Reis, J. Pharm. Biomed. Anal. 42, 423 (2006)

    CAS  PubMed  Google Scholar 

  53. J.M. Calatayud, C.G. Benito, Anal. Chim. Acta 231, 259 (1990)

    Google Scholar 

  54. J.M. Pulgarín, L.G. Bermejo, Anal. Chim. Acta 333, 59 (1996)

    Google Scholar 

  55. V.A. Pedrosa, D. Lowinsohn, M. Bertotti, Electroanal. Int. J. Devoted Fundam. Pract. Asp. Electroanal. 18, 931 (2006)

    CAS  Google Scholar 

  56. Z. Xu, Q. Yue, Z. Zhuang, D. Xiao, Microchim. Acta 164, 387 (2009)

    CAS  Google Scholar 

  57. G.G. Oliveira, B.C. Janegitz, M.B. Batistão, F.H. Salami, O. Fatibello-Filho, O.D. Leite, Quim. Nova 32, 1755 (2009)

    CAS  Google Scholar 

  58. W. Ruengsitagoon, S. Liawruangrath, A. Townshend, Talanta 69, 976 (2006)

    CAS  PubMed  Google Scholar 

  59. K.G. Kumar, R. Letha, J. Pharm. Biomed. Anal. 15, 1725 (1997)

    CAS  PubMed  Google Scholar 

  60. G. Burgot, F. Auffret, J.-L. Burgot, Anal. Chim. Acta 343, 125 (1997)

    CAS  Google Scholar 

  61. A.K. Baytak, S. Duzmen, T. Teker, M. Aslanoglu, Mater. Sci. Eng. C 57, 164 (2015)

    CAS  Google Scholar 

  62. L. Ahmadpour-Mobarakeh, A. Nezamzadeh-Ejhieh, Mater. Sci. Eng. C 49, 493 (2015)

    CAS  Google Scholar 

  63. S.H. Lee, J.H. Lee, V.-K. Tran, E. Ko, C.H. Park, W.S. Chung, G.H. Seong, Sens. Actuators B Chem. 232, 514 (2016)

    CAS  Google Scholar 

  64. F. Cao, Q. Dong, C. Li, J. Chen, X. Ma, Y. Huang, D. Song, C. Ji, Y. Lei, Sens. Actuators B Chem. 256, 143 (2018)

    CAS  Google Scholar 

  65. Y. Xu, W. Lei, J. Su, J. Hu, X. Yu, T. Zhou, Y. Yang, D. Mandler, Q. Hao, Electrochim. Acta 259, 994 (2018)

    CAS  Google Scholar 

  66. B. Habibi, M. Jahanbakhshi, M. Abazari, J. Iran. Chem. Soc. 11, 511 (2014)

    CAS  Google Scholar 

  67. M.A. Oliva, R.A. Olsina, A.N. Masi, Talanta 66, 229 (2005)

    Google Scholar 

  68. E. Llorent-Martínez, D. Šatínský, P. Solich, P. Ortega-Barrales, A. Molina-Díaz, J. Pharm. Biomed. Anal. 45, 318 (2007)

    PubMed  Google Scholar 

  69. H. Montaseri, P.B. Forbes, Mater. Today Commun. 17, 480 (2018)

    CAS  Google Scholar 

  70. M. Locatelli, R. Cifelli, C. Di Legge, R.C. Barbacane, N. Costa, M. Fresta, C. Celia, C. Capolupo, L. Di Marzio, J. Chromatogr. A 1388, 79 (2015)

    CAS  PubMed  Google Scholar 

  71. M.-H. Langlois, A. Vekris, C. Bousses, E. Mordelet, N. Buhannic, C. Séguard, P.-O. Couraud, B.B. Weksler, K.G. Petry, K. Gaudin, J. Chromatogr. B 988, 20 (2015)

    CAS  Google Scholar 

  72. N.W. Ali, M. Gamal, M. Abdelkawy, Arab. J. Chem. 10, S1868 (2017)

    CAS  Google Scholar 

  73. H. Khajehsharifi, Z. Eskandari, A. Asadipour, Drug Test. Anal. 2, 162 (2010)

    CAS  PubMed  Google Scholar 

  74. S. Sharifian, A. Nezamzadeh-Ejhieh, Mater. Sci. Eng. C 58, 510 (2016)

    CAS  Google Scholar 

  75. M. Taei, M.S. Jamshidi, Microchem. J. 130, 108 (2017)

    CAS  Google Scholar 

  76. C. Li, J. Xu, Y. Wu, Y. Zhang, C. Zhang, W. Lei, Q. Hao, J. Electroanal. Chem. 824, 52 (2018)

    CAS  Google Scholar 

  77. N.H. Phong, T.T.T. Toan, M.X. Tinh, T.N. Tuyen, T.X. Mau, D.Q. Khieu, J. Nanomater. 2018, 1–15 (2018)

    Google Scholar 

  78. M. Gioia, P. Andreatta, S. Boschetti, R. Gatti, J. Pharm. Biomed. Anal. 48, 331 (2008)

    CAS  PubMed  Google Scholar 

  79. B. Tsvetkova, I. Pencheva, P. Peikov, Afr. J. Pharm. Pharmacol. 6, 1332 (2012)

    CAS  Google Scholar 

  80. F. Ibrahim, N. El-Enany, R.N. El-Shaheny, I.E. Mikhail, Anal. Sci. 31, 943 (2015)

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chemistry Department, College of Science, Salahadin University, Erbil, Iraq

    Hunar Yasin Muhammad & Azad Tawfiq Faizullah

Authors
  1. Hunar Yasin Muhammad
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Azad Tawfiq Faizullah
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hunar Yasin Muhammad.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Muhammad, H.Y., Faizullah, A.T. Double injection–single detector flow injection spectrophotometric method for simultaneous determination of ascorbic acid and paracetamol in pharmaceutical formulations. J IRAN CHEM SOC 17, 111–118 (2020). https://doi.org/10.1007/s13738-019-01758-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13738-019-01758-4

Keywords

Search

Navigation