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Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds

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  • Tissue Culture/Biotechnology
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Abstract

Dendrobium candidum is an important medicinal plant rich in polysaccharides, phenols, flavonoids and its bioactive compounds are known for various pharmacological activities. Natural populations of D. candidum are depleting due to overexploitation and increasing demand for medicinal and health products. An efficient protocol for protocorm suspension cultures of Dendrobium candidum in shake flasks was developed and the effect of auxins, basal media, nitrogen concentration, sucrose concentration, organic supplements like banana homogenate, coconut water and potato extract were investigated for the production of biomass and bioactive compounds. For the production of polysaccharides, polyphenolics, and flavonoids, the optimized culture conditions were found to be MS basal medium supplemented with 0.5 mg·L−1 NAA, 5:25 mM NH4:NO3 as nitrogen source, 2.5% (w/v) sucrose, and 1% (v/v) banana homogenate. Results of the current study could be used for scale-up process.

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Literature Cited

  • Arditti, J. and R. Ernst. 1993. Microporpagation of orchids. John Wiley & Song, New York p. 609–619.

    Google Scholar 

  • Bao, X.S., Q.S. Shun, and L.Z. Chen. 2001. The medicinal plants of Dendrobium (Shi-Hu) in China, a coloured atlas. Fudan University Press, Shanghai.

    Google Scholar 

  • Baque, M.A., E.J. Lee, and K.Y. Paek. 2010. Medium salt strength induced changes in growth, physiology and secondary metabolite content in adventitious roots of Morinda citrifolia: The role of antioxidant enzymes and phenylalanine ammonia lyase. Plant Cell Rep. 29:685–694.

    Article  Google Scholar 

  • Chu, C.C. 1978. The N6 medium and its applications to anther culture of cereal crops, p. 43–50. In: Proc. symp. plant tissue culture. Science Press, Beijing.

    Google Scholar 

  • Cui, H.Y., H.N. Murthy, S.H. Moh, Y.Y. Cui, E.J. Lee, and K.Y. Paek. 2014. Production of biomass and bioactive compounds in protocorm cultures of Dendrobium candidum Wall ex Lindl. using balloon type bubble bioreactors. Ind. Crop Prod. 53:28–33.

    Article  CAS  Google Scholar 

  • Cui, X.H., H.N. Murthy, Y.X. Jin, Y.H. Yim, J.Y. Kim, and K.Y. Paek. 2011. Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift bioreactor. Bioresour. Technol. 102:10072–10079.

    Article  CAS  PubMed  Google Scholar 

  • Fujita, Y., Y. Hara, T. Ogino, and C. Suga. 1981. Production of shikonin derivatives by cell suspension cultures of Lithospermum erythrorhizon: I. Effects of nitrogen sources on the production of shikonin derivatives. Plant Cell Rep. 1:59–60.

    Article  CAS  PubMed  Google Scholar 

  • Gao, R., S.Q. Wu, X.C. Piao, S.Y. Park, and M.L. Lian. 2014. Micropropagation of Cymbidium sinense using continuous and temporary airlift bioreactor systems. Acta Physiol. Plant. 36:117–124.

    Article  CAS  Google Scholar 

  • Gamborg, O.L., R.A. Miller, and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158.

    Article  CAS  PubMed  Google Scholar 

  • Hellwig, S., J. Drossard, R.M. Twyman, and R. Fischer. 2004. Plant cell cultures for the production of recombinant proteins. Nat. Biotechnol. 22:1415–1422.

    Article  CAS  PubMed  Google Scholar 

  • Hossain, M.M., M. Sharma, and P. Pathak. 2013. In vitro propagation of Dendrobium aphyllum (Orchidaceae)-seed germination to flowering. J. Plant Biochem. Biotechnol. 22:157–167.

    Article  Google Scholar 

  • Knudson, L. 1946. A new nutrient solution for the germination of orchid seed. Am. Orchid Soc. Bull. 14:214–217.

    Google Scholar 

  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15:473–497.

    Article  CAS  Google Scholar 

  • Murthy, H.N., E.J. Lee, and K.Y. Paek, K.Y. 2014. Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell Tiss. Organ Cult. 118:1–16.

    Article  CAS  Google Scholar 

  • Murthy, H.N. and N. Praveen. 2013. Carbon source and medium pH affects the growth of Withania somnifera (L.) Dunal adventitious roots and withanolide-A production. Nat. Prod. Res. 27:185–189.

    Article  CAS  PubMed  Google Scholar 

  • Murthy, H.N. and N. Praveen. 2012. Influence of macro-elements and nitrogen source on adventitious root growth and withanolide-A production in Withania somnifera (L.) Dunal. Nat. Prod. Res. 26:466–473.

    Article  CAS  PubMed  Google Scholar 

  • Nakagawa, K., A. Konagai, H. Fukui, and H. Tabata. 1984. Release and crystallization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep. 3:254–257.

    Article  CAS  PubMed  Google Scholar 

  • Nagella, P. and H.N. Murthy. 2011. Effects of macroelements and nitrogen source on biomass accumulation and withanolide-A production from cell suspension cultures of Withania somnifera (L.) Dunal. Plant Cell Tiss. Organ Cult. 104:119–124.

    Article  CAS  Google Scholar 

  • Nagella, P., I.M. Chung, and H.N. Murthy. 2011. In vitro production of gymnemic acid from cell suspension culture of Gymnema sylvestre R. Br. Eng. Life Sci. 11:537–540.

    Article  CAS  Google Scholar 

  • Naik, P.M., S.H. Manohar, N. Praveen, and H.N. Murthy. 2010. Effect of sucrose and pH levels on in vitro shoot regeneration form leaf explants of Bacopa monnieri and accumulation of bacoside-A in regenerated shoots. Plant Cell Tiss. Organ Cult. 100:235–239.

    Article  Google Scholar 

  • Ng, T.B., J. Liu, J.H. Wong, X. Ye, S.C.W. Sze, Y. Tong, and K.Y. Zhang. 2012. Review of research on Dendrobium, a prized folk medicine. Appl. Microbiol. Biotechnol. 93:1795–1803.

    Article  CAS  PubMed  Google Scholar 

  • Ono, N.N. and L. Tian. 2010. The multiplicity of hairy root cultures: Prolific possibilities. Plant Sci. 180:439–446.

    Article  PubMed  Google Scholar 

  • Paek, K.Y. and H.N. Murthy. 2002. Temperate oriental Cymbidium species, p. 235–286. In: T. Kull and J. Arditti (eds.). Orchid biology: Reviews and perspectives, Vol. VIII. Kulwer Academic Publisher, Dordrecht.

    Chapter  Google Scholar 

  • Park, S.Y., H.N. Murthy, and K.Y. Paek. 2000. Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tiss. Organ Cul. 63:67–72.

    Article  Google Scholar 

  • Praveen, N. and H.N. Murthy. 2013. Withanolide A production from Withania somnifera hairy root cultures with improved growth by altering the concentrations of macro elements and nitrogen source in the medium. Acta Physiol. Plant. 35:811–816.

    Article  CAS  Google Scholar 

  • Praveen, N. and H.N. Murthy. 2012. Synthesis of withanolide A depends on carbon source and medium pH in hairy root cultures of Withania somnifera. Ind. Crops Prod. 35:241–243.

    Article  CAS  Google Scholar 

  • Praveen, N., H.N. Murthy, and I.M. Chung. 2011. Improvement of growth and gymnemic acid production by altering the macroelements concentration and nitrogen source supply in cell suspension cultures of Gymnema sylvestre R. Br. Ind. Crops Prod. 33:282–286.

    Article  CAS  Google Scholar 

  • Rao, R.S. and G.A. Ravishankar. 2002. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv. 20:10–153.

    Google Scholar 

  • Schenk, R.U. and A.C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledons and dicotyledonous plant cell cultures. Can. J. Bot. 50:199–204.

    Article  CAS  Google Scholar 

  • Slinkard, K. and V.L. Singleton. 1977. Total phenol analysis: automation and comparison with manual methods. Am. J. Enol.Viticul. 28:49–55.

    CAS  Google Scholar 

  • Vacin, E. and F.W. Went. 1949. Some pH changes in nutrient solutions. Bot. Gaz. 110:605–613.

    Article  CAS  Google Scholar 

  • Wang, Y. and Y. Liu. 2006. Cryopreservation of ornamental plant germplasm. Plant Physiol. Commun. 42:559–566.

    Google Scholar 

  • White, P.R. 1963. The cultivation of animal and plant cells. 2nd ed. Ronald Press, New York.

    Google Scholar 

  • Winarto, B., F. Rachmawati, A. Santi, and J.A. Teixeira da Silva. 2013. Mass propagation of Dendrobium ‘Zahar FR 62’, a new hybrid used for cut flowers, using bioreactor culture. Sci. Hort. 161:170–180.

    Article  CAS  Google Scholar 

  • Zha, X.Q., L.P. Luo, S.T. Jiang, and J.H. Wang. 2007. Enhancement of polysaccharide production in suspension cultures of protocorm-like bodies form Dendrobium huoshanense by optimization of medium compositions and feeding of sucrose. Process Biochem. 42:344–351.

    Article  CAS  Google Scholar 

  • Zhao, P., W. Wang, F.S. Feng, F. Wu, and Z.Q. Yang. 2007. High frequency shoot regeneration through transverse thin cell layer culture in Dendrobium candidum Wall. ex Lindl. Plant Cell Tiss. Organ Cult. 90:131–139.

    Article  Google Scholar 

  • Zheng, Y. 2005. Shihu in China pharmacopeia. Chemical Technology, Beijing p. 61–63.

    Google Scholar 

  • Zhuang, X.P., Y.Y. Lu, and G.S. Yang. 1992. Extraction and determination of flavonoid in gingko. Chin. Herb. Med. 23:122–124.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Korea

    Hai-Yan Cui, Hosakatte Niranjana Murthy & Kee-Yoeup Paek

  2. Department of Botany, Karnatak University, Dharwad, 580003, India

    Hosakatte Niranjana Murthy

  3. Antiageing Research Institute, Incheon, 406-840, Korea

    Sang Hyun Moh

  4. School of Agriculture and Food Science, Zheijian Agriculture and Forestry University, Lin’an, Hangzjpi, 311300, China

    Yong-Yi Cui

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  1. Hai-Yan Cui
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  2. Hosakatte Niranjana Murthy
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  3. Sang Hyun Moh
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  4. Yong-Yi Cui
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  5. Kee-Yoeup Paek
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Correspondence to Kee-Yoeup Paek.

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Cui, HY., Murthy, H.N., Moh, S.H. et al. Establishment of protocorm suspension cultures of Dendrobium candidum for the production of bioactive compounds. Hortic. Environ. Biotechnol. 56, 114–122 (2015). https://doi.org/10.1007/s13580-015-0082-5

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  • DOI: https://doi.org/10.1007/s13580-015-0082-5

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