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Highly expressed TLX1NB and NPSR1-AS1 lncRNAs could serve as diagnostic tools in colorectal cancer

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Abstract

Colorectal cancer is the main cause of human death due to cancer. This fact could be due to the insufficiency of early diagnosis or poor therapeutic strategies. Various molecular tools have been utilized in studies to assess their potentials as diagnostic biomarkers or determining factors in precision medicine. Among these molecules, long non-coding RNAs (lncRNA) have been emerging as accurate and potent transcripts to improve the detection of cancer. The overexpressed lncRNAs could also be deeply studied as the molecules for the targeted therapy in different malignancies, in particular colorectal cancer. Thus, we utilized an unbiased approach to select the up-regulated lncRNAs in colon adenocarcinoma via analyzing the TCGA dataset. Then, we validated the overexpression of two first-ranked lncRNAs, i.e., NPSR1-AS1 and TLX1NB, in our in-house colorectal cancer samples as compared to the paired adjacent normal tissues. The analyses revealed that these lncRNAs could significantly distinguish the tumor against the normal samples. The results may have implications in the early diagnosis and targeted therapy of colorectal cancer.

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Acknowledgements

We would like to appreciate the tissue donors for this study.

Funding

Not applicable.

Author information

Author notes

  1. Shaghayegh Dastjerdi and Mehdi Valizadeh equally contributed to this work.

Authors and Affiliations

  1. Gene Raz Bu Ali, Genetic and Biotechnology Academy, Isfahan, Iran

    Shaghayegh Dastjerdi, Nasrin Fattahi Dolatabadi & Atefeh Zamani

  2. Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Shaghayegh Dastjerdi

  3. Unit of Genomics Research, Digestive Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran

    Mehdi Valizadeh

  4. Department of Internal Medicine, School of Medicine and Allied Medical Sciences, Digestive Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran

    Rasoul Nemati

  5. Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

    Mohammad Amin Honardoost & Hossein Tabatabaeian

  6. Anahid Cancer Clinic, Isfahan Healthcare City, Isfahan, Iran

    Hossein Tabatabaeian

Authors
  1. Shaghayegh Dastjerdi
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  2. Mehdi Valizadeh
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  3. Rasoul Nemati
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  4. Mohammad Amin Honardoost
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  5. Nasrin Fattahi Dolatabadi
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  6. Atefeh Zamani
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  7. Hossein Tabatabaeian
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Corresponding author

Correspondence to Hossein Tabatabaeian.

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All authors declare that they have no conflict of interest.

Research involving human participants and/or animals

All the samples were collected in accordance with the guidelines issued by the Ethics Committee of Isfahan University of Medical Sciences (approval number: 6593769), regarding the 64th World Medical Association General Assembly of Helsinki declaration amended in October 2013.

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The informed consent was taken from all the patients who participated in this study.

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Dastjerdi, S., Valizadeh, M., Nemati, R. et al. Highly expressed TLX1NB and NPSR1-AS1 lncRNAs could serve as diagnostic tools in colorectal cancer. Human Cell 34, 1765–1774 (2021). https://doi.org/10.1007/s13577-021-00597-x

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  • DOI: https://doi.org/10.1007/s13577-021-00597-x

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