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An increased disulfide/native thiol ratio and oxidative stress index in metabolic syndrome patients with postprandial lipemia

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Abstract

Background

Metabolic syndrome (MetS) is closely related to lipid disorders and increased oxidant stress, and is associated with cardiovascular diseases.

Objective

The purpose of this research was to examine thiol/disulfide homeostasis and oxidative stress in MetS patients with postprandial lipemia (PPL) during fasting by considering time-dependent changes in the postprandial period.

Methods

Twenty-five patients with MetS and 25 healthy controls underwent a 6-h oral fat tolerance test. Dynamic thiol/disulfide homeostasis (native thiol, disulfide, and total thiol) values and total oxidant status (TOS), total antioxidant status (TAS), and Oxidative Stress Index (OSI): (TOS/TAS) were evaluated.

Results

Increased levels of disulfide, and higher disulfide/native thiol ratio, TOS, and OSI values were observed at fasting and in the postprandial period in MetS compared to the control group, peaking at the 4th hour in both groups (p < 0.05). ROC analysis showed that both fasting and 4th hour disulfide/native thiol ratios exhibited the highest values. Higher disulfide/native thiol ratio values were observed at the 4th hour and higher OSI in the 2nd hour in the upper tertiles for MetS (p < 0.05).

Conclusions

An increased disulfide/native thiol ratio and OSI level elevation in MetS may be closely associated with PPL. The disulfide/native thiol ratio in MetS subjects with PPL may play a role for evaluating oxidative stress, especially in postprandial 4th hour.

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Acknowledgment

The authors would like to thank the Karadeniz Technical University, Faculty of Medicine, and Department of Medical Biochemistry, where this study was performed. We are also indebted to all those individuals who agreed to participate in the research.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon, Turkey

    Serap Ozer Yaman, Fulya Balaban Yucesan, Asım Orem, Birgul Vanizor Kural & Huseyin Yaman

  2. Department of Cardiology, Faculty of Medicine, Karadeniz Technical University, 61080, Trabzon, Turkey

    Cihan Orem

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  1. Serap Ozer Yaman
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Contributions

S.O.Y., A.O., and F.B.Y. planned and designed this research. S.O.Y., A.O., and C.O. conducted the research. S.O.Y. and F.B.Y. performed and conceived the experiments. S.O.Y., F.B.Y., and H.Y. analyzed the data. S.O.Y., A.O., F.B.Y., and B.V.K. wrote the manuscript. All authors read and approved the final version.

Corresponding author

Correspondence to Serap Ozer Yaman.

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Ethics approval

Approvals from the local Ethics Committee were obtained (Submission Number 2021/142, dated May 5, 2021).

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Before enrolling in this study, written informed consent was obtained from all participants (both control and MetS groups).

Competing interests

The authors declare no competing interests.

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Ozer Yaman, S., Balaban Yucesan, F., Orem, A. et al. An increased disulfide/native thiol ratio and oxidative stress index in metabolic syndrome patients with postprandial lipemia. Int J Diabetes Dev Ctries 43, 125–133 (2023). https://doi.org/10.1007/s13410-022-01095-y

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