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In vitro antioxidant and antibacterial activities of ethanolic extracts from the leaves and stems of Oudneya Africana R. growing in the El Oued (Algeria)

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Abstract

This work is a component of the development of the aerial parts (leaves and stems) of Oudneya africana R., of the Brassicaceae family. For that purpose, the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activities (DPPH, ABTS, β-carotene, and phenanthroline assays), and antimicrobial activity of the ethanolic extracts of this plant were evaluated. Ethanolic leaf extract had a higher quantity of phenolics (69.75 ± 1.87 μg GAE/mg) and flavonoids (91.88 ± 1.94 μg QE/mg) than stem extract. Despite their significant antioxidant activities, leaf and stem ethanolic extracts had a weaker capacity to block the DPPH radical, as well as the ABTS radical, the β-carotene-linoleate bleaching assay, and the phenanthroline test, than industrial antioxidants, namely butylhydroxytoluene (BHT) and butylhydroxyanisole (BHA). The remarkable antioxidant activity was observed in ethanolic stem extract on the ABTS assay with an IC50 value of 15.90 μg/mL and in the β-carotene with an IC50 value of 20.21 μg/mL. The ethanolic extract from leaves and stems showed excellent antibacterial activity against Candida albicans, with inhibition diameters of 13 mm and 12 mm, respectively. This work comprises a preliminary investigation, and as such, it suggests Oudneya africana R. as a potential candidate for having antibacterial and antioxidant properties and also draws attention to the potential value of using this plant as a traditional treatment.

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Acknowledgments

This research has been funded by Scientific Research Deanship at University of Ha’il - Saudi Arabia through project number <<RG-23 014>>.

Funding

This research has been funded by Scientific Research Deanship at University of Ha’il - Saudi Arabia through project number <<RG-23 014>>.

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Authors and Affiliations

  1. Faculty of Exact Sciences, Chemistry Department, El Oued University, P.O. Box 789, 39000, El Oued, Algeria

    Salah Neghmouche Nacer, Ammar Zobeidi & Ammar Haouat

  2. Biotechnology Research Center, UV 03, P.O. Box E73, Ali Mendjeli, Constantine, 25000, Algeria

    Chawki Bensouici

  3. Faculty of Technology, Process Engineering Department, El Oued university, P.O. Box 789, 39000, El Oued, Algeria

    Mohammed Larbi Ben Amor

  4. CHEMS Research Unit, University of the Mentouri Brothers of Constantine 1, 25000, Constantine, Algeria

    Fadila Louafi

  5. Faculty of Sciences of Sfax, Organic Chemistry Laboratory (LR17ES08), University of Sfax, 3029, Sfax, Tunisia

    Younes Moussaoui & Ridha Ben Salem

  6. Faculty of Sciences of Gafsa, University of Gafsa, 2112, Gafsa, Tunisia

    Younes Moussaoui

  7. Research Institute of Sciences and Engineering (RISE), University of Sharjah, Sharjah, 27272, United Arab Emirates

    Muhammad Imran Khan

  8. Chemical Engineering Department, College of Engineering, University of Ha’il, P.O. Box 2440, Ha’il, 81441, Saudi Arabia

    Djamel Ghernaout & Noureddine Elboughdiri

  9. Chemical Engineering Department, Faculty of Engineering, University of Blida, P.O. Box 270, 09000, Blida, Algeria

    Djamel Ghernaout

  10. Chemical Engineering Process Department, National School of Engineers Gabes, University of Gabes, Street Omar ibn El-Khattab, 6029, Gabes, Tunisia

    Noureddine Elboughdiri

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  1. Salah Neghmouche Nacer
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Contributions

Conceptualization: S.N.N., M.I.K. and C.B.; methodology: A.Z. and A.H.; software: M.L.B.A., N.E., and D.G.; validation: A.H., C.B. M.I.K., and Y.M.; formal analysis: N.E., F.L., and C.B.; investigation: S.N.N. and N.E.; resources: A.Z., R.B.S., and S.N.N.; data curation: S.N.N.; writing—original draft preparation: N.E, S.N.N., C.B.; writing—review and editing: D.G., S.N.N., and Y.M.; visualization: R.B.S. and A.Z.; supervision: N.E. and F.L. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Noureddine Elboughdiri.

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Nacer, S.N., Zobeidi, A., Bensouici, C. et al. In vitro antioxidant and antibacterial activities of ethanolic extracts from the leaves and stems of Oudneya Africana R. growing in the El Oued (Algeria). Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04856-9

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