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Antibiofilm activity of biosurfactant produced by a sponge-associated marine Cobetia sp. JCG-23

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Abstract

Marine symbiotically associated microbes play a vital role and are an excellent source of natural compounds that exert wide biological activities. In this study, we have reported on the identification, characterization, phylogenetic relationship, and anti-biofilm surface-active compound-producing abilities of marine invertebrate sponge-associated Cobetia sp. JCG-23. Among 24 isolates, a total of five strains (JCG2, JCG19, JCG20, JCG22, and JCG23) have active surface molecule producing potential on the emulsification index assay. Interestingly, the potential candidate JCG-23, produces biosurfactants with low surface tension (22 Nm-1) that exert anti-biofilm activity against Pseudomonas aeruginosa PAO1. The isolate was identified as genus Cobetia sp. JCG-23 with 99.1% sequence similarity to Cobetia crustatorum (EU909460) based on 16S rRNA gene sequence analysis. The large-scale production, purification, stability, and characterization of biosurfactant were carried out and its surface activity was determined using the oil drop method. Subsequent spectral analysis such as UV, FT-IR, and GC-MS analysis indicated that the purified biosurfactant was a hydroxyl fatty acid, namely octadecanoic acid (C18H36O2) with a molecular weight of 284 m/z. Furthermore, the effect of antibiofilm activity on the viability of Pseudomonas aeruginosa PAO1 by static ring tube and light and confocal laser scanning microscopy analysis revealed that the octadecanoic acid from Cobetia sp. JCG-23 has strong biofilm dismantle ability against Pseudomonas aeruginosa PAO1. Further characterization of the biosurfactant from the isolate Cobetia sp. JCG-23 can pave the way for developing novel bioactive agents targeting biofilm-forming pathogens on topical and medical devices.

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Funding

The authors would like to thank the Department of Chemistry, Sri Shanmugha College of Engineering and Technology, Salem 637304, Tamil Nadu, India for their constant support for this work. The authors acknowledge the funding of SBT, Madurai Kamaraj University, 625021, Tamil Nadu and providing equipment support as institutional infrastructure. The authors would like to acknowledge the late Prof. Dr SRD Jebakumar for his valuable guidance.

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Authors and Affiliations

  1. Department of Chemistry, Sri Shanmugha College of Engineering and Technology, Salem, Tamil Nadu, 637304, India

    Govindarajan Ganesan

  2. Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India

    Chandrasekar Balu & Samuel Raj Babu Arulmani

  3. Department of Physics, Government college of Engineering, Dharmapuri, Tamil Nadu, 6367041, India

    Suganthi Ganesan

  4. Department of Biotechnology, PSGR Krishnammal College for Women, 641004, Coimbatore, Tamil Nadu, India

    Sabariswaran Kandasamy

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  1. Govindarajan Ganesan
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Contributions

Govindarajan Ganesan: supervision, writing—original draft and conceptualization. Chandrasekar Balu performed the experiments. Suganthi Ganesan: technical correction and validation. Samuel Raj Babu Arulmani: writing—review and editing. Sabariswaran Kandasamy: writing—review and editing.

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Ganesan, G., Balu, C., Ganesan, S. et al. Antibiofilm activity of biosurfactant produced by a sponge-associated marine Cobetia sp. JCG-23. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04808-3

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