Abstract
A large number of animal by-products including the bones, skins, tendons, scales, and feet are being generated around the world. These by-products are produced by meat processing facilities, slaughterhouses, and fish processing units. These are often put into landfills without any treatment, thus causing environmental pollution. However, these by-products can be used for the production of valuable compounds such as collagen and gelatin that can be used in different food products. Gelatin is the most widely used hydrocolloid in the world. By the year 2025, the market size of gelatin will exceed ~ $5 billion and is predicted to reach $6.7 billion in 2027. Therefore, gelatin recovery is beneficial both economically and environmentally. Commercially available gelatin is derived from porcine and bovine bones and skins. There are several other by-products from which gelatin can be extracted including by-products from chickens, ducks, fish, and camels. The increase in sustainable and green technologies that eliminate the usage of toxic solvents and less time for extraction has increased the demand for novel extraction processes in the food industries. The application of more recently developed techniques can be used for the extraction of gelatin to overcome several limitations of the traditional process. The present review discusses the application of conventional (hot water bath) and advanced technologies (ultrasound, microwave, or enzyme-assisted extraction) to extract the gelatin from different animal by-products. The physicochemical properties of gelatin depend on the source, extraction conditions, and pretreatment methods. These advanced techniques improve the functional properties, quality, and process efficiency of gelatin.
Similar content being viewed by others
Data availability
Not applicable.
References
Gautam K, Vishvakarma R, Sharma P, Singh A, Gaur VK, Varjani S, Srivastava JK (2022) Production of biopolymers from food waste: constrains and perspectives. Bioresour Technol 367:127650
Kumar V, Sharma N, Umesh M, Selvaraj M, Al-Shehri BM, Chakraborty P, Duhan L, Sharma S, Pasrija R, Lakkaboyana AMK, SR, (2022) Emerging challenges for the agro-industrial food waste utilization: a review on food waste biorefinery. Bioresour Technol 362:127790
Ominski K, McAllister T, Stanford K, Mengistu G, Kebebe EG, Omonijo F, Cordeiro M, Legesse G, Wittenberg K (2021) Utilization of by-products and food waste in livestock production systems: a Canadian perspective. Anim Front 11:55–63
Nayak A, Bhushan B (2019) An overview of the recent trends on the waste valorization techniques for food wastes. J Environl Manage 233:52–370
Toldrá F, Mora L, Reig M (2022) Current developments in meat by-products. In New Aspects of Meat Quality (pp. 649–665). Woodhead Publishing United Kingdom.
Plazzotta S, Manzocco L (2019) Food waste valorization. In Saving Food (pp. 279–313). Academic Press. https://doi.org/10.1016/B978-0-12-815357-4.00010-9.
Usman M, Sahar A, Inam-Ur-Raheem M, Rahman UU, Sameen A, Aadil RM (2022) Gelatin extraction from fish waste and potential applications in food sector. Int J Food Sci Technol 57:154–163
Gutierrez IR, Tovar AK, Godínez LA (2018) Sustainable sorbent materials obtained from orange peel as an alternative for water treatment. In T. Yonar (ed.), Wastewater and water quality, IntechOpen, London. 201–218 https://doi.org/10.5772/intechopen.76137.
Rigueto CVT, Rosseto M, Alessandretti I, de Oliveira R, Wohlmuth DAR, Menezes JF, Loss RA, Dettmer A, Pizzutti IR (2022) Gelatin films from wastes: a review of production, characterization, and application trends in Food Preservation and Agriculture. Food Res Int 162:112114
Liu D, Nikoo M, Boran G, Zhou P, Regenstein JM (2015) Collagen and gelatin. Annu Rev Food Sci Technol 6:527–557
Tukiran NA (2019) Analytical methods for gelatin differentiation. JHIS 2:1–10
Mehta MJ, Bharmoria P, Bhayani K, Kumar A (2017) Gelatin solubility and processing in ionic liquids: an approach towards waste to utilization. ChemistrySelect 2:9895–9900
Boran G, Regenstein JM (2009) Optimization of gelatin extraction from silver carp skin. J Food Sci 74:432–441
Ali AMM, Kishimura H, Benjakul S (2018) Physicochemical and molecular properties of gelatin from skin of golden carp (Probarbus jullieni) as influenced by acid pretreatment and prior-ultrasonication. Food Hydrocoll 82:164–172
Bahar A, Kusumawati N (2021) Comparison of the physico-chemical properties of type-B halal gelatin from bovine and goat skin material. In IOP Conf Ser.: Earth Environ Sci 709:012033
Uddin SMK, Hossain MM, Sagadevan S, Al-Amin M, Johan MR (2021) Halal and kosher gelatin: applications as well as detection approaches with challenges and prospects. Food Biosci 44:101422
Abedinia A, Nafchi AM, Sharifi M, Ghalambor P, Oladzadabbasabadi N, Ariffin F, Huda N (2020) Poultry gelatin: characteristics, developments, challenges, and future outlooks as a sustainable alternative for mammalian gelatin. Trends Food Sci Technol 104:14–26
Bichukale A, Koli J, Sonavane A, Vishwasrao V, Pujari K, Shingare P (2018) Functional properties of gelatin extracted from poultry skin and bone waste. IJAB 6:87–101
Chakka AK, Muhammed A, Sakhare P, Bhaskar N (2017) Poultry processing waste as an alternative source for mammalian gelatin: extraction and characterization of gelatin from chicken feet using food grade acids. Waste Biomass Valorization 8:2583–2593
Ai-Temimi WKA, Kalaf A, Imran RA, Alsamir ZA (2021) Enzymatic and thermal extraction of gelatin from fish scales and study its sensory properties. Int J Pharm Res 13:1043–1054
Jamal P, Jaswir I, Yusof N, Jami M (2017) Novel method for gelatin extraction of various local fish using high pressure processing (HPP). Int Food Res J 24:533–539
Mirzapour-Kouhdasht A, Sabzipour F, Taghizadeh MS, Moosavi-Nasab M (2019) Physicochemical, rheological, and molecular characterization of colloidal gelatin produced from common carp by-products using microwave and ultrasound-assisted extraction. J Texture Stud 50:416–425
Mirzapour Kouhdasht A, Moosavi-Nasab M, Aminlari M (2018) Gelatin production using fish wastes by extracted alkaline protease from Bacillus licheniformis. J Food Sci Technol 55:5175–5180
Abdelfadeel HFA (2012) Extraction and characterization of gelatin from melon bug (Aspongubus viduatus) and sorghum bug (Agonoscelis pubescens) for application in to ice cream making. Thesis, Sudan University for Science & Technology, Khartoum, Sudan, MSc
Mariod AA (2020) Watermelon bug (Aspongopus viduatus) as a source of edible oil, protein, and gelatin. In African edible insects as alternative source of food, oil, protein and bioactive components. 159–168. Cham, Switzerland: Springer.
Mariod AA, Fadul H (2013) Gelatin, source, extraction and industrial applications. Acta Sci Pol Technol Aliment 12:135–147
Islam MR, Yuhi T, Meng D, Yoshioka T, Ogata Y, Ura K, Takagi Y (2021) Purity and properties of gelatins extracted from the head tissue of the hybrid kalamtra sturgeon. LWT-Food Sci Technol 142:110944
Tümerkan ETA, Cansu U, Boran G, Regenstein JM, Özoğul F (2019) Physiochemical and functional properties of gelatin obtained from tuna, frog and chicken skins. Food Chem 287:273–279
Karim AA, Bhat R (2009) Fish gelatin: properties, challenges, and prospects as an alternative to mammalian gelatins. Food Hydrocoll 23:563–576
Hou PZ, Regenstein JM (2004) Optimization of extraction conditions for pollock skin gelatin. J Food Sci 69:393–398
Ahmad T, Ismail A, Ahmad SA, Khalil KA, Kumar Y, Adeyemi KD, Sazili AQ (2017) Recent advances on the role of process variables affecting gelatin yield and characteristics with special reference to enzymatic extraction: a review. Food Hydrocoll 63:85–96
Kittiphattanabawon P, Benjakul S, Sinthusamran S, Kishimura H (2016) Gelatin from clown featherback skin: extraction conditions. LWT-Food Sci Technol 66:186–192
Huang T, Tu ZC, Xinchen-Shangguan WH, Zhang L, Sha XM (2017) Rheological and structural properties of fish scales gelatin: effects of conventional and ultrasound-assisted extraction. Int J Food Prop 20:1210–1220
Kumar DP, Chandra MV, Elavarasan K, Shamasundar B (2017) Structural properties of gelatin extracted from croaker fish (Johnius sp) skin waste. Int J Food Prop 20:2612–2625
Duthen S, Levasseur-Garcia C, Kleiber D, Violleau F, Vaca-Garcia C, Tsuchikawa S, Raynaud CD, Daydé J (2021) Using near-infrared spectroscopy to determine moisture content, gel strength, and viscosity of gelatin. Food Hydrocoll 115:106627
Boran G, Regenstein JM (2010) Fish gelatin. Adv Food Nutr Res 60:119–143
Alipal J, Pu’ad NM, Lee T, Nayan N, Sahari N, Basri H, Idris M, Abdullah H (2021) A review of gelatin: properties, sources, process, applications, and commercialisation. Mater Today: Proc 42:240–250
Gómez-Guillén M, Giménez B, López-Caballero MA, Montero M (2011) Functional and bioactive properties of collagen and gelatin from alternative sources: a review. Food Hydrocoll 25:1813–1827
Nagarajan M, Benjakul S, Prodpran T, Songtipya P, Kishimura H (2012) Characteristics and functional properties of gelatin from splendid squid (Loligo formosana) skin as affected by extraction temperatures. Food Hydrocoll 29:389–397
Erge A, Zorba O (2018) Optimization of gelatin extraction from chicken mechanically deboned meat residue using alkaline pre-treatment. LWT-Food Sci Technol 97:205–212
Sila A, Martinez-Alvarez O, Krichen F, Gómez-Guillén MC, Bougatef A (2017) Gelatin prepared from European eel (Anguilla anguilla) skin: physicochemical, textural, viscoelastic and surface properties. Colloids Surf A: Physicochem Eng Asp 529:643–650
Kaewruang P, Benjakul S, Prodpran T, Nalinanon S (2013) Physicochemical and functional properties of gelatin from the skin of unicorn leatherjacket (Aluterus monoceros) as affected by extraction conditions. Food Biosci 2:1–9
Misra N, Martynenko A, Chemat F, Paniwnyk L, Barba FJ, Jambrak AR (2018) Thermodynamics, transport phenomena, and electrochemistry of external field-assisted nonthermal food technologies. Crit Rev Food Sci Nutr 58:1832–1863
Marić M, Grassino AN, Zhu Z, Barba FJ, Brnčić M, Brnčić SR (2018) An overview of the traditional and innovative approaches for pectin extraction from plant food wastes and by-products: ultrasound-, microwaves-, and enzyme-assisted extraction. Trends Food Sci Technol 76:28–37
Noor NQIM, Razali RS, Ismail NK, Ramli RA, Razali UHM, Bahauddin AR, Zaharudin N, Rozzamri A, Bakar J, Shaarani SM (2021) Application of green technology in gelatin extraction: a review. Processes 9:2227
Roselló-Soto E, Galanakis CM, Brnčić M, Orlien V, Trujillo FJ, Mawson R, Knoerzer K, Tiwari BK, Barba FJ (2015) Clean recovery of antioxidant compounds from plant foods, by-products and algae assisted by ultrasounds processing. Modeling approaches to optimize processing conditions. Trends Food Sci Technol 42:134–149
Chemat F, Rombaut N, Meullemiestre A, Turk M, Perino S, Fabiano-Tixier AS, Abert-Vian M (2017) Review of green food processing techniques. Preservation, transformation, and extraction. Innov Food Sci Emerg Technol 41:357–377
Senarathna PDS, Marapana RAUJ (2021) Comparative analysis of the effect of ultrasound-assisted and conventional water bath extraction methods on the physicochemical characteristics of tilapia scales gelatin. J Aquat Food Prod Technol 30:893–906
Karnjanapratum S, Benjakul S (2020) Asian bullfrog (Rana tigerina) skin gelatin extracted by ultrasound-assisted process: characteristics and in-vitro cytotoxicity. Int J Biol Macromol 148:391–400
Asih I, Kemala T, Nurilmala M (2019) Halal gelatin extraction from Patin fish bone (Pangasius hypophthalmus) by-product with ultrasound-assisted extraction. IOP Conf Ser.: Earth Environ Sci 299:2061
Ahmad T, Ismail A, Ahmad SA, Khalil KA, Awad EA, Leo TK, Imlan JC, Sazili AQ (2018) Characterization of gelatin from bovine skin extracted using ultrasound subsequent to bromelain pretreatment. Food Hydrocoll 80:264–273
Ahmad T, Ismail A, Ahmad SA, Khalil KA, Leo TK, Awad EA, Imlan JC, Sazili AQ (2018) Effects of ultrasound assisted extraction in conjugation with aid of actinidin on the molecular and physicochemical properties of bovine hide gelatin. Molecules 23:730
Tu ZC, Huang T, Wang H, Sha XM, Shi Y, Huang XQ, Man ZZ, Li DJ (2015) Physico-chemical properties of gelatin from bighead carp (Hypophthalmichthys nobilis) scales by ultrasound-assisted extraction. J Food Sci Technol 52:2166–2174
Widyasari R, Rawdkuen S (2014) Extraction and characterization of gelatin from chicken feet by acid and ultrasound assisted extraction. Food Appl Biosci J 2:85–97
Sarfarazi M, Jafari SM, Rajabzadeh G, Galanakis CM (2020) Evaluation of microwave-assisted extraction technology for separation of bioactive components of saffron (Crocus sativus L.). Ind Crops Prod 145:111978
Kim TK, Ham YK, Shin DM, Kim HW, Jang HW, Kim YB, Choi YS (2020) Extraction of crude gelatin from duck skin: effects of heating methods on gelatin yield. Poult Sci 99:590–596
Liu T, Dai H, Ma L, Yu Y, Tang M, Li Y, Hu W, Feng X, Zhang Y (2019) Structure of Hyla rabbit skin gelatin as affected by microwave-assisted extraction. Intl J Food Prop 22:1594–1607
Park JH, Choe JH, Kim HW, Hwang KE, Song DH, Yeo EJ, Kim HY, Choi YS, Lee SH, Kim CJ (2013) Effects of various extraction methods on quality characteristics of duck feet gelatin. Food Sci Anim Resour 33:162–169
Rossi E, Efendi R, Rahmayuni MSB, Yoenissa R (2022) Utilization of waste mixed pangasius fish fillet and pineapple core to produce peptone for lactic acid bacteria growth media. Int J Vet Sci 11:272–279
Mena-García A, Ruiz-Matute AI, Soria AC, Sanz ML (2019) Green techniques for extraction of bioactive carbohydrates. TrAC-Trends Anal Chem 119:115612
Nadar SS, Rao P, Rathod VK (2018) Enzyme assisted extraction of biomolecules as an approach to novel extraction technology: a review. Food Res Int 108:309–330
Matulessy DN, Erwanto Y, Nurliyani N, Suryanto E, Abidin MZ, Hakim TR (2021) Characterization and functional properties of gelatin from goat bone through alcalase and neutrase enzymatic extraction. Vet World 14:2397
Gaspar-Pintiliescu A, Stefan LM, Anton ED, Berger D, Matei C, Negreanu-Pirjol T, Moldovan L (2019) Physicochemical and biological properties of gelatin extracted from marine snail Rapana venosa. Mar Drugs 17:589
Abedinia A, Ariffin F, Huda N, Nafchi AM (2017) Extraction and characterization of gelatin from the feet of Pekin duck (Anas platyrhynchos domestica) as affected by acid, alkaline, and enzyme pretreatment. Int J Biol Macromol 98:586–594
Ahmad T, Ismail A, Ahmad SA, Khalil KA, Kee LT, Awad EA, Sazili AQ (2020) Extraction, characterization and molecular structure of bovine skin gelatin extracted with plant enzymes bromelain and zingibain. J Food Sci Technol 57:3772–3781
Ahmad T, Ismail A, Ahmad SA, Khalil KA, Teik Kee L, Awad EA, Sazili AQ (2019) Physicochemical characteristics and molecular structures of gelatin extracted from bovine skin: effects of actinidin and papain enzymes pretreatment. Int J Food Prop 22:138–153
Badii F, Howell NK (2006) Fish gelatin: structure, gelling properties and interaction with egg albumen proteins. Food Hydrocoll 20:630–640
Scopel S, Restelatto D, Baldasso C, Dettmer A, Santana RM (2019) Food Hydrocooloids Steam explosion as pretreatment to increase gelatin extraction yield from chromium tanned leather wastes. Environ Prog Sustain Energy 38:367–373
Rigueto CVT, Rosseto M, Gomes KS, Loss RA, Biduski B, Manera C, Godinho M, Brião VB, Dettmer A, Pizzutti IR (2023) Steam explosion pretreatment for bovine limed hide waste gelatin extraction. Food Hydrocoll 142:108854
Funding
This work was supported by the National Institute of Food Science & Technology, University of Agriculture, Faisalabad, Pakistan.
Author information
Authors and Affiliations
Contributions
Muhammad Usman: investigation—equal, writing—original draft—equal, writing—review and editing—equal. Anum Ishaq: methodology—equal, writing—review and editing—equal. Joe Mac Regenstein: investigation—equal, writing—review and editing—equal. Amna Sahar: investigation—equal, writing—review and editing—equal, supervision. Rana Muhammad Aadil: writing—review and editing—equal, supervision. Aysha Sameen: writing—review and editing—equal, conceptualization—equal. Muhammad Issa Khan: writing—review and editing—equal. Amna Alam: writing—review and editing—equal.
Corresponding author
Ethics declarations
Ethical approval
Not applicable.
Conflict of interest
The authors declare no competing interests.
Additional information
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Usman, M., Ishaq, A., Regenstein, J.M. et al. Valorization of animal by-products for gelatin extraction using conventional and green technologies: a comprehensive review. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04547-5
Received:
Revised:
Accepted:
Published:
DOI: https://doi.org/10.1007/s13399-023-04547-5