Abstract
Developing the most reliable and eco-friendly techniques for nanoparticle synthesis is an emerging step in green nanotechnology. Intending to avoid the potential risks of harmful substances for a safe environment, research on environmentally friendly methods of generating metal/metal oxide nanoparticles (NP) is increasing. In this study, the aqueous leaf extract of Elephantopus scaber was used to create bimetallic nanoparticles (Ag-ZnO), and the synthesized Ag-ZnO NPs were characterized for their physical and chemical properties. Different spectroscopic and microscopic methods, including UV–visible spectroscopy, FTIR, scanning electron microscopy (SEM), EDX, and XRD, were used to characterize the synthesized Ag-ZnO NPs. SEM revealed the nanoparticles’ irregular shape, and FTIR demonstrated that secondary metabolites served as capping and stabilising agents during the production of the particles. The antioxidant activity of biosynthesised bimetallic nanoparticles was determined using DPPH, hydroxyl and reducing power assays. The E. scaber-mediated Ag-ZnO NPs were tested for their antibiofilm, antibacterial, and antioxidant effects and are reported. The biosynthesised nanoparticles showed significant antioxidant activity of 62% at 500 µg/ml concentration. The results of antibacterial activity showed that MIC of biosynthesised Ag-ZnO NPs was 0.125 μg/ml against Staphylococcus aureus and Bacillus subtilis. The results of studying the antibiofilm activity of Ag-ZnO NPs showed that it was concentration-dependent. Results obtained from the present study indicate that the biosynthesised Ag-ZnO NPs using E. scaber leaf extract are eco-friendly and can be used as a good antibacterial, antibiofilm and antioxidant agent.
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References
Tariq M, Mohammad KN, Ahmed B, Siddiqui MA, Lee J (2022) Biological synthesis of silver nanoparticles and prospects in plant disease management. Molecules 27(15):4754. https://doi.org/10.3390/molecules27154754
Ramos RMCR, Regulacio MD (2021) Controllable synthesis of bimetallic nanostructures using biogenic reagents: a green perspective. ACS Omega 6(11):7212–7228. https://doi.org/10.1021/acsomega.1c00692
Sharma G, Kumar Amit, Sharma S, Naushad Mu, Dwivedi RP, AlOthman ZA, Mola GT (2017) Novel development of nanoparticles to bimetallic nanoparticles and their composites: a review. J King Saud Univ- Sci 31(2):257–269
Shafey AME (2020) Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: a review. Green Process Synth 9(1):304–339. https://doi.org/10.1515/gps-2020-0031
John M (2002) The history of India (Greenwood Histories of Modern Nations) Green Wood Press; Annotated edition.
Ramkumar KM, Rajaguru P, Ananthan R (2007) Antimicrobial properties and phytochemical constituents of an antidiabetic plant Gymnema montanum. Adv Biol Res 1(1–2):67–71
Ho WY, Yeap SK, Ho CL, Raha AR, Suraini AA, Alitheen NB (2011) Elephantopus scaber induces cytotoxicity in MCF-7 human breast cancer cells via p53- induced apoptosis. J Med Pl Res 5(24):5741–5749
Blois MS (1958) Antioxidant determination by the use of a stable free radical. Nature 181:1199–1200
Halliwell B, Gutteridge JMC (1999) Free radicals in biology and medicine, 3rd edn. Oxford University Press, Oxford, UK
Kumar RS, Hemalatha S (2011) In vitro antioxidant activity of alcoholic leaf extract and subfractions of Alangium lamarckii Thwaites. J Chem Pharm Res 3:259–267
Ansari MA, Khan HM, Khan AA, Cameotra SS, Alzohairy MA (2015) Anti-biofilm efficacy of silver nanoparticles against MRSA and MRSE isolated from wounds in a tertiary care hospital. Indian J Med Microbiol 33:101–109
Selvaraj A, Jayasree T, Valliammai A, Pandian SK (2019) Myrtenol attenuates MRSA biofilm and virulence by suppressing sarA expression dynamism. Front Microbil 10:2027. https://doi.org/10.3389/fmcib.2019.02027
Blando F, Russo R, Negro C, De Bellis L, Frassinetti S (2019) Antimicrobial and antibiofilm activity against Staphylococcus aureus of Opuntia ficus-indica (L.) Mil.l cladode polyphenolic extracts. Antioxidants 8(5):117. https://doi.org/10.3390/antiox8050117
Singh P, Kim YJ, Wang C, Mathiyalagan R, El-AgamyFarh M, Yang DC (2016) Biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications. Artific Cells Nanomed Biotechnol 44(3):811–816. https://doi.org/10.3109/21691401.2015.1008514
Krajczewski J, Kołątaj K, Kudelski A (2017) Plasmonic nanoparticles in chemical analysis. RSC Adv 7(28):17559–17576. https://doi.org/10.1039/C7RA01034F
Zare M, Namratha K, Alghamdi S, Mohammad YHE, Hezam A, Zare M, Drmosh QA, Byrappa K, Chandrashekar BN, Ramakrishna S, Zhang X (2019) Novel green biomimetic approach for synthesis of ZnO-Ag nanocomposite; antimicrobial activity against food-borne pathogen, biocompatibility and solar photocatalysis. Sci Rep 9(1):8303. https://doi.org/10.1038/s41598-019-44309-w
Krithika R, Balasasirekha R (2021) FTIR spectrum and XRD of postbiotics-exopolysaccharides zinc oxide nanoparticles. J Adv Sci Res12 (3) Suppl 2: 292–300
Alomari AA, Kloub Fares KE, Moustafa NE (2018) Green synthesis of assembled silver nanoparticles in nano capsules of Peganum harmala L leaf extract Antibacterial activity and conjugate investigation. Cogent Chem 4(1):1532374. https://doi.org/10.1080/23312009.2018.1532374
Azizi M, Sedaghat S, Tahvildari K, Derakhshi P, Ghaemi A (2017) Synthesis of silver nanoparticles using Peganum harmala extract as a green route. Green Chem Lett Rev 10(4):420–427. https://doi.org/10.1080/17518253.2017.1395081
Banerjee P, Satapathy M, Mukhopahayay A, Das P (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterisation, antimicrobial property and toxicity analysis. Bioresour Bioprocessing 1(3):1–10. https://doi.org/10.1186/s40643-014-0003-y
Hiremath R, Jalalpure S, Pethakar S (2016) Chromatographic fingerprint analysis of hydro-alcoholic extract of medicinally important plant Elephantopus scaber l. Using HPTLC technique. Indian J Pharmaceut Educ Res 50(4):689–694. https://doi.org/10.5530/ijper.50.4.21
Kharat SN, Mendhulkar VD (2016) Synthesis, characterisation and studies on antioxidant activity of silver nanoparticles using Elephantopus caber leaf extract. Mat Sci Eng C 62:719–724. https://doi.org/10.1016/j.msec.2016.02.024
Contreras-Guzman ES, Strong FC III (1982) Determination of tocopherols (vitamin E) by reduction of cupric ion. J Asso Off Anal Chem 65(5):1215–1221. https://doi.org/10.1093/jaoac/65.5.1215
Perillo B, Di Donato M, Pezone A, Di Zazzo E, Giovannelli P, Galasso G, Castoria G, Migliaccio A (2020) ROS in cancer therapy: the bright side of the moon. Experiment Mole Med 52(2):192–203. https://doi.org/10.1038/s12276-020-0384-2
Murray CJ, Ikuta KS, Sharara F, Swetschinski L, Aguilar GR, Gray A, Han C, Bisignano C, Rao P, Wool E, Johnson SC (2022) Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis. The Lancet 399(10325):629–655. https://doi.org/10.1016/S0140-6736(21)02724-0
O'Neill J (2016) Tackling drug-resistant infections globally: final report and recommendations. Publisher: Government of the United Kingdom 84
Alexander J (2009) History of the medical use of silver. Surg Infect 10:289–292. https://doi.org/10.1089/sur.2008.9941
Frei A, Verderosa AD, Elliott AG, Zuegg J, Blaskovich MAT (2023) Metals to combat antimicrobial resistance. Nature Rev Chem. 7(3):02-224. https://doi.org/10.1038/s41570-023-00463-4
Chandrangsu P, Rensing C, Helmann JD (2017) Metal homeostasis and resistance in bacteria. Nature Rev Microbiol 15(6):338–350. https://doi.org/10.1038/nrmicro.2017.15
Padilla-Cruz AL, Garza-Cervantes JA, Vasto-Anzaldo XG, Rivas GG, Bultimea AL, Ramirez JRM (2021) Synthesis and design of Ag–Fe bimetallic nanoparticles as antimicrobial synergistic combination therapies against clinically relevant pathogens. Sci Rep 11:5351. https://doi.org/10.1038/s41598-021-84768-8
Padmavathy N, Vijayaraghavan R (2011) Interaction of ZnO nanoparticles with microbes—a physio and biochemical assay. J Biomed Nanotechnol 7(6):813–822. https://doi.org/10.1166/jbn.2011.1343
Azizi S, Mohamad R, Rahim RA, Moghaddam AB, Moniri M, Ariff A, Saad WZ, Namvab F (2016) ZnO-Ag core shell nanocomposite formed by green method using essential oil of wild ginger and their bactericidal and cytotoxic effects. Appl Surf Sci 384:517–524. https://doi.org/10.1016/j.apsusc.2016.05.052
Matai I, Sachdev A, Dubey P, Kumar SU, Bhushan B, Gopinath P (2014) Antibacterial activity and mechanism of Ag–ZnO nanocomposite on S. aureus and GFP-expressing antibiotic resistant E. coli. Colloids Surf B: Biointerfaces 115:359–367. https://doi.org/10.1016/j.colsurfb.2013.12.005
Rai MK, Deshmukh SD, Ingle AP, Gade AK (2012) Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. J Appl Microbial 112(5):841–852. https://doi.org/10.1111/j.1365-2672.2012.05253.x
Gopinath K, Gowri S, Arumugam A (2013) Phytosynthesis of silver nanoparticles using Pterocarpus santalinus leaf extract and their antibacterial properties. J Nanostr Chem 3:68
Wang L, Hu C, Shao L (2017) The antimicrobial activity of nanoparticles: present situation and prospects for the future. Internat J Nanomed 12:227. https://doi.org/10.2147/IJN.S121956
Gurunathan S, Han JW, Kwon DN, Kim JH (2014) Enhanced antibacterial and anti-biofilm activities of silver nanoparticles against Gram-negative and Gram-positive bacteria. Nanoscale Res Lett 9(1):1–17
Cha SH, Hong J, McGuffie M, Yeom B, VanEpps JS, Kotov NA (2015) Shape-dependent biomimetic inhibition of enzyme by nanoparticles and their antibacterial activity. ACS Nano 9(9):9097–9105. https://doi.org/10.1021/acsnano.5b03247
Cruz DM, Mostafavi E, Vernet-Crua A, Barabadi H, Shah V, Cholula-Díaz JL, Guisbiers G, Webster TJ (2020) Green nanotechnology-based zinc oxide (ZnO) nanomaterials for biomedical applications: a review. J Phys Mater 3(3):034005. https://doi.org/10.1088/2515-7639/ab8186. (034005)
Korshed P, Li L, Liu Z, Mironov A, Wang T (2019) Size-dependent antibacterial activity for laser-generated silver nanoparticles. J Interdiscipl Nanomed 4(1):24–33. https://doi.org/10.1002/jin2.54
Franco D, Calabrese G, Guglielmino SPP, Conoci S (2022) Metal-based nanoparticles: antibacterial mechanisms and biomedical application. Microorganisms 10(9):1778. https://doi.org/10.3390/microorganisms10091778
Kavitha A, Doss A, Pole RP, Rani TKP, Prasad R, Satheesh S (2023) A mini review on plant-mediated zinc oxide nanoparticles and their antibacterial potency. Biocatal Agri Biotechnol 48:102654
Moore MN (2006) Do nanoparticles present ecotoxicological risks for the health of the aquatic environment? Environ Int 32:967–976. https://doi.org/10.1016/j.envint
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Suresh, P., Doss, A., Selvi, G.S.A. et al. Antibiofilm, antibacterial and antioxidant activity of biofabricated bimetallic (Ag-ZnO) nanoparticles from Elephantopus scaber L. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-04230-9
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DOI: https://doi.org/10.1007/s13399-023-04230-9