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In silico optimization of anthocyanin extraction from gladious flower extracts and evaluation of its antioxidant potential

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Abstract

Gladiolus (Gladiolus grandiflora Hort.) is one of the important horticultural crops belonging to Iridaceae family. It grows in flowerbeds, borders, and containers and has immense potential as cut flowers. In this study, we have optimized the anthocyanin extraction from gladiolus Pusa Suahagin (PS) flower using one-variable-at-a-time (OVAT) and response surface methodology (RSM) methods. The optimum conditions for maximum anthocyanin content of 40.45 g−1 DW was obtained considering the water:hydrochloride (1.8:0.6) ratio, temperature: 52.72 °C, time: 2 h and solid loading: 7% (w/v). MS studies confirmed the presence of pelargonidin, cyanidin, delphinidin, peonidin, petunidin, and malvidin compounds in PS flower extract. Free radical scavenging capability determined by DPPH assay of PS flower extract showed 84.63%, which implies that the PS flower extract can be used as colorant or nutraceutical in food industry and potential agent for enhancement of vase life pertinent to cut flowers in flower industry.

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Acknowledgements

The authors are grateful to the Director, ICAR-Directorate of Floricultural Research, Pune for facilitating the research work.

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Authors and Affiliations

  1. Department of Biotechnology, Vignan’s Foundation for Science, Technology & Research, Vadlamudi, Guntur, 522213, Andhra Pradesh, India

    Anjani Devi Chintagunta, N. S. Sampath Kumar & Janaki Kolla

  2. ICAR-Directorate of Floricultural Research, 411306, Pune, Maharashtra, India

    Ganesh B. Kadam, P. Naveen Kumar, K. V. Prasad & S. P. Jeevan Kumar

  3. ICAR-National Research Centre for Grapes, 412307, Pune, Maharashtra, India

    Ahammed Shabeer & Nasiruddin Shaikh

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  1. Anjani Devi Chintagunta
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Contributions

ADC conducted experiments and evaluated results, NSSK reviewed literature and analyzed the data, JK conducted the experiments, GBK cultivated the PS flower and supplied the flowers on time, PNK meticulously edited the paper, AS and NS conducted MS studies and analyzed the data of flower sample, KVP meticulously edited the MS and SPJK conceived the idea, analyzed, the data and drafted the paper.

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Correspondence to S. P. Jeevan Kumar.

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Chintagunta, A.D., Kumar, N.S.S., Kolla, J. et al. In silico optimization of anthocyanin extraction from gladious flower extracts and evaluation of its antioxidant potential. Biomass Conv. Bioref. (2022). https://doi.org/10.1007/s13399-022-03653-0

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