Abstract
This study investigated for the first time the production of β-glucosidase from Penicillium roqueforti ATCC 10110 by solid-state fermentation using the forage palm (Nopalea cochenillifera) as a substrate. For the optimization of incubation parameters, time, and temperature, the central composite rotational statistical model was used, resulting in an increase in enzymatic activity, by 94.70% with a maximum yield of 935.07 ± 21.70 IU/g at 23 °C and 56% moisture content. The β-glucosidase produced to show higher reactivity and stability at pH 5.0 at 52 °C. In addition to good halotolerance, maintaining 139.93% and 95.34% of the residual activity in the reaction and 57.93% and 94.28% after 24 h of incubation. The addition of Na+, Fe2 +, Mg2+, EDTA, Triton X-100, lactose, and dichloromethane improved β-glucosidase activity, while Ca2+, Zn2+, and Co2+ were not expressed, and SDS, ethanol, acetone, and methanol were inhibitors. Therefore, it was possible to produce β-glucosidase with a halotolerant profile, indicating a promising alternative to obtain this enzyme for biotechnological and industrial applications.
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The authors would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for their financial support and the State University of Santa Cruz (UESC) for its administrative and technical support.
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das Neves, C.A., de Menezes, L.H.S., Soares, G.A. et al. Production and biochemical characterization of halotolerant β-glucosidase by Penicillium roqueforti ATCC 10110 grown in forage palm under solid-state fermentation. Biomass Conv. Bioref. 12, 3133–3144 (2022). https://doi.org/10.1007/s13399-020-00930-8
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DOI: https://doi.org/10.1007/s13399-020-00930-8