Abstract
Effects of concentration on the rheological behavior of acylated pepsin-solubilized collagen solutions were investigated by steady shear tests, dynamic frequency sweep, creep tests and thixotropic loop measurements in this paper. The results showed that both acylated collagen and native collagen solutions exhibited the typical pseudoplastic behavior and displayed shear thinned behavior with the increase of shear rate. With the increase of acylated collagen concentrations from 5 to 10 mg/mL, shear viscosity, elasticity modulus (G′), viscous modulus (G″), complex viscosity (η*), and the ability to resist deformation increased due to the physical entanglement, whilst loss tangent (tan δ) decreased. Additionally, with the increase of acylated collagen concentrations, the area of thixotropic loop increased from 6.94 to 44.40 watts/m3, indicating that the thixotropy of acylated collagen increased. Compared with native collagen solution, acylated collagen solution had stronger shear viscosity, η*, thixotropy, and ability to resist deformation. Furthermore, Power law model, Carreau model, Cross model, Leonov model and Burger model, were suitable for the fitting of the experimental data.
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Adams, M.L., A. Lavasanifar, and G.S. Kwon, 2003, Amphiphilic block copolymers for drug delivery, J. Pharm. Sci. 92, 1343–1355.
Avery, N.C. and A.J. Bailey, 2006, The effects of the Maillard reaction on the physical properties and cell interactions of collagen, Pathol. Biol. 54, 387–395.
Chen, Y.H., M. Zhang, W.T. Liu, and G.Y. Li, 2011, Properties of alkali-solubilized collagen solution crosslinked by N-hydroxysuccinimide activated adipic acid, Korea-Aust. Rheol. J. 23, 41–48.
Choung, S.H., M.-S. Chun, and C.Y. Kim, 2011, Single molecule tracking of a semiflexible polyelectrolyte chain in solvent under uniform electroosmotic flows, J. Korean Phys. Soc. 59, 2847–2854.
de Paula, M., G. Goissis, V.C.A. Martins, and J.C. da Silva Trindade, 2005, Injectable gels of anionic collagen: Rhamsan composites for plastic correction: Preparation, characterization, and rheological properties, J. Biomed. Mater. Res. Part B: Appl. Biomater. 75B, 393–399.
Dobraszczyk, B.J. and M.P. Morgenstern, 2003, Rheology and the breadmaking process, J. Cereal. Sci. 38, 229–245.
Dolz, M., M.J. Hernándea, J. Delegido, and J. Muñoz, 2007, Influence of xanthan gum and locust bean gum upon flow and thixotropic behavior of food emulsions containing modified starch, J. Food Eng. 81, 179–186.
Duan, L., J. Li, C. Li, and G. Li, 2013, Effects of NaCl on the rheological behavior of collagen solution, Korea-Aust. Rheol. J. 25, 137–144.
Ferry, J.D., 1989, Viscoelastic Properties of Polymers, 3rd Ed., Wiley, New York, p.369.
Friess, W., 1998, Collagen-biomaterial for drug delivery, Eur. J. Pharm. Biopharm. 45, 113–136.
Friess, W. and M. Schlapp, 2001, Effects of processing conditions on the rheological behavior of collagen dispersions, Eur. J. Pharm. Biopharm. 51, 259–265.
Friess, W. and M. Schlapp, 2006, Sterilization of tentamicin containing collagen/PLGA microparticle composites, Eur. J. Pharm. Biopharm. 63, 176–187.
Gebhard, S., 2000, A Practical Approach to Rheology and Rheometry, 2nd Ed., Gebrueder HAAKE GmbH, Karlsruhe.
Hsieh, T.T., C. Tiu, G.P. Simon, and R.Y. Wu, 1999, Rheology and miscibility of thermotropic liquid crystalline polymer blends, J. Non-Newton. Fluid Mech. 86, 15–35.
Kao, N., S.N. Bhattacharya, R., Shanks, and L.H. Coopes, 1998, The effect of temperature on the viscoelastic properties of model and industrial dispersion, J. Rheol. 42, 493–506.
Karaman, S., M.T. Yilmaz, H. Cankurt, A. Kayacier, and O. Sagdic, 2012, Linear creep and recovery analysis of ketchup-processed cheese mixtures using mechanical simulation models as a function of temperature and concentration, Food Res. Int. 48, 507–519.
Kasapis, S. and J.R. Mitchell, 2001, Definition of the rheological glass transition temperature in association with the concept of iso-free-volume, Int. J. Biol. Macromol. 29, 315–321.
Kataoka, K., G.S. Kwon, M. Yokoyama, T. Okano, and Y. Sakurai, 1993, Block copolymer micelles as vehicles for drug delivery, J. Control. Release 24, 119–132.
Kim, J.-S. and J.W. Park, 2004, Characterization of acid-soluble collagen from pacific whiting surimi processing byproducts, J. Food Sci. 69, 637–642.
Korhonen, M., L. Hellen, J. Hirvonen, and J. Yliruusi, 2001, Rheological properties of creams with four different surfactant combinations-effect of storage time and conditions, Int. J. Pharm. 221, 187–196.
Lai, G.L., Z.L. Du, and G.Y. Li, 2007, The rheological behaviour of collagen dispersion/poly (vinyl alcohol) blends, Korea-Aust. Rheol. J. 19, 81–88.
Lapasin, R. and S. Pricl, 1995, Rheology of Industrial Polysaccharides: Theory and Applications, 1st Ed., Blackie Academic & Professional, London.
Lee, C.H., A. Singla, and Y. Lee, 2001, Biomedical applications of collagen, Int. J. Pharm. 221, 1–22.
Leonov, A.I., 1990, On the rheology of filled polymers, J. Rheol. 34, 1039–1068.
Li, C., W., Liu, L. Duan, Z. Tian, and G. Li, 2014, Surface activity of pepsin-solubilized collagen acylated by lauroyl chloride along with succinic anhydride, J. Appl. Polym. Sci. 131, 40174–40181.
Li, C., H. Tian, L. Duan, Z. Tian, and G. Li, 2013, Characterization of acylated pepsin-solubilized collagen with better surface activity, Int. J. Biol. Macromolec. 57, 92–98.
Li, C., Z. Tian, W. Liu, and G. Li, 2015, Structural properties of pepsin-solubilized collagen acylated by lauroyl chloride along with succinic anhydride, Mater. Sci. Eng. C: Mater. Biol. Appl. 55, 327–334.
Moresi, M., S.L. Presti, and M. Mancini, 2001, Rheology of scleroglucan dispersion, J. Food Eng. 50, 235–245.
Nik, W.B.W., F.N. Ani, H.H. Masjuki, and S.G.E. Giap, 2005, Rheology of bio-edible oils according to several rheological models and its potential as hydraulic fluid, Ind. Crop. Prod. 22, 249–255.
Stoodley, P., Z. Lewandowski, J.D. Boyle, and H.M. Lappin-Scott, 1999, Structural deformation of bacterial biofilms caused by short-term fluctuations in fluid shear: An in situ investigation of biofilm rheology, Biotechnol. Bioeng. 65, 83–92.
Yang, Y.L. and L.J. Kaufman, 2009, Rheology and confocal reflectance microscopy as probes of mechanical properties and structure during collagen and collagen/hyaluronan self-assembly, Biophys. J. 96, 1566–1585.
Yoshimura, K., M. Terashima, D. Hozan, and K. Shirai, 2000, Preparation and dynamic viscoelasticity characterization of alkali-solubilized collagen from shark skin, J. Agric. Food Chem. 48, 685–690.
Zhang, Z.K., G.Y. Li, and B. Shi, 2006, Physicochemical properties of collagen, gelatin and collagen hydrolysate derived from bovin limed split wasts, J. Soc. Leath. Tech. Ch. 90, 23–28.
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Li, C., Duan, L., Tian, Z. et al. Rheological behavior of acylated pepsin-solubilized collagen solutions: Effects of concentration. Korea-Aust. Rheol. J. 27, 287–295 (2015). https://doi.org/10.1007/s13367-015-0028-6
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DOI: https://doi.org/10.1007/s13367-015-0028-6