Abstract
Congenital human cytomegalovirus (HCMV) infection causes sensorineural hearing loss (SNHL) and other neurological disorders, although the neuropathogenesis of HCMV infection is not well understood. Here, we show that the expression of SLITRK6, one of causative genes for hereditary SNHL, was robustly downregulated by HCMV infection in cultured neural cells. We also show that HCMV-encoded immediate-early 2 (IE2) proteins mediate this downregulation and their carboxy-terminal region, especially amino acid residue Gln548, has a critical role. These findings suggest that the downregulation of SLITRK6 expression by IE2 may have a role in HCMV-induced SNHL and other neurological disorders.
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Acknowledgments
We thank M. Iikura for the excellent technical assistance. We also thank N. Inoue for providing the reagents.
This work was partly supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan (24591616) to H.N., the Ministry of Health, Labour and Welfare of Japan (H23-Jisedai-Ippan-001) to H.N., and the Grants of National Center for Child Health and Development (24-17 and 27-18 to H.N., 25-9 to S.F.).
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Liao, H., Sato, H., Chiba, R. et al. Human cytomegalovirus downregulates SLITRK6 expression through IE2. J. Neurovirol. 23, 79–86 (2017). https://doi.org/10.1007/s13365-016-0475-y
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DOI: https://doi.org/10.1007/s13365-016-0475-y