Abstract
In this study, we attempted a population survey of spiders in peach orchards with and without pesticide application; however, we had difficulty discriminating them. To identify spiders, we determined the nucleotide sequences of a ca. 640-bp region in the mitochondrial cytochrome oxidase subunit I (COI) gene from 17 species belonging to eight families. To identify five wolf spiders (Araneae: Lycosidae), which are the most abundant spiders in peach orchards in Okayama Prefecture, PCR and restriction fragment length polymorphism were developed on the basis of the COI gene sequences. Population survey results suggest that pesticide applications adversely affect spider populations in peach orchards.
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Acknowledgments
This study was supported by grants from the Ministry of Agriculture, Forestry and Fisheries (MAFF), the Wesco Scientific Promotion Foundation, and the Ohara Foundation for Agricultural Research. The authors also thank A. Tanikawa (The University of Tokyo) for identifying the samples.
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Sonoda, S., Yamashita, J., Kohara, Y. et al. Population survey of spiders using mt-DNA (COI) sequences in Japanese peach orchards. Appl Entomol Zool 46, 81–86 (2011). https://doi.org/10.1007/s13355-010-0011-0
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DOI: https://doi.org/10.1007/s13355-010-0011-0