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Fabrication and characterization of bilayer scaffolds made of decellularized dermis/nanofibrous collagen for healing of full-thickness wounds

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Abstract

Skin tissue engineering has progressed from simple wound dressings to biocompatible materials with desired physico-chemical properties that can deliver regenerative biomolecules. This study describes using a novel biomimetic hybrid scaffold of decellularized dermis/collagen fibers that can continuously deliver stromal cell-derived factor-1 alpha (SDF-1α) for skin regeneration. In diabetic rat models, the idea that sustained SDF-1α infusion could increase the recruitment of CXCR4-positive cells at the injury site and improve wound regeneration was investigated. The morphology of the scaffold, its biocompatibility, and the kinetics of SDF-1 release were all assessed. SDF-1α was successfully incorporated into collagen nanofibers, resulting in a 200-h continuous release profile. The microscopic observations exhibited that cells are attached and proliferated on proposed scaffolds. As evaluated by in vivo study and histological examination, fabricated scaffold with SDF-1α release capacity exhibited a remarkably more robust ability to accelerate wound regeneration than the control group. Besides, the SDF-1α-loaded scaffold demonstrated functional effects on the proliferation and recruitment of CD31 and CXCR4-positive cells in the wound bed. Additionally, no adverse effects such as hyperplasia or scarring were found during the treatment period. It may be concluded that the fabricated hybrid scaffold based on natural polymer opens up a new option for topical administration of bioactive molecules. We believe the SDF-1α-loaded hybrid scaffold has promise for skin tissue engineering.

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The data sets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

Not applicable.

Funding

This work was supported by the Iran University of Medical Sciences (IUMS) through research Grant No.1397–01-117–33032 and the Qom University of Medical Sciences (MUQ) through research Grant No. IR. MUQ. REC. 1398. 121.

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Author notes

  1. Alireza Rezapour and Peiman Brouki Milan have contributed equally to this work and should be considered the corresponding joint author.

Authors and Affiliations

  1. Cellular and Molecular Research Centre, Iran University of Medical Sciences, Tehran, Iran

    Naser Amini, Ahmad Hivechi, Mazaher Gholipourmalekabadi, Ahvan Nasrolahi & Peiman Brouki Milan

  2. Department of Textile Engineering, Amirkabir University of Technology, Tehran, Iran

    Ahmad Hivechi

  3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Shiva Asadpour

  4. Department of Neurosurgery, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Kaveh Ebrahimzadeh

  5. Tissue Engineering Research Group (TERG), Department of Anatomy and Cell Biology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Saeid Kargozar

  6. Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mazaher Gholipourmalekabadi

  7. Department of Tissue Engineering and Regenerative Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

    Mazaher Gholipourmalekabadi, Melina Ghasemian, Masoud Mozafari & Peiman Brouki Milan

  8. Department of Mechanical Engineering, Arak University of Technology, Arak, Iran

    Amir Shafaat

  9. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, ON, Canada

    Masoud Mozafari

  10. Cellular and Molecular Research Centre, Qom University of Medical Sciences, Qom, Iran

    Alireza Rezapour

  11. Department of Tissue Engineering and Regenerative Medicine, School of Medicine, Qom University of Medical Sciences, Qom, Iran

    Alireza Rezapour

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  1. Naser Amini
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Contributions

NA, AS, and AH wrote the main manuscript, MGM and MM reviewed the manuscript. SA and SK carried out data analysis. KE and MG prepared figures and validated experiments. AN prepared the supplementary figures. AR and PBM supervised the project and wrote the part of the manuscript with the insights of all other authors. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Peiman Brouki Milan or Alireza Rezapour.

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All institutional and national guidelines for the care and use of laboratory animals were followed. All animal experiments were performed in compliance with guidelines approved by the Animal Use and Care Administrative Advisory Committee at the University of Iran, Tehran, Iran (Ethical code No. IR.IUMS.REC.1398.752). Furthermore, all animal experiments comply with the National Institutes of Health guide for the care and use of laboratory animals (NIH Publications No. 8023, revised 1978).

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Amini, N., Hivechi, A., Asadpour, S. et al. Fabrication and characterization of bilayer scaffolds made of decellularized dermis/nanofibrous collagen for healing of full-thickness wounds. Drug Deliv. and Transl. Res. 13, 1766–1779 (2023). https://doi.org/10.1007/s13346-023-01292-0

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