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The role of corchorus in spreading of tomato yellow leaf curl virus on tomato in Jeddah, Saudi Arabia

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Abstract

Corchorus (Corchorus capsularis L. and Corchorus olitorius L.) is one of the most important fiber crops grown in tropical and subtropical regions throughout the world. Field survey was conducted and naturally infected leaf samples were collected from corchorus and tomato plants in Jeddah, Saudi Arabia. The causal virus was transmitted by whiteflies to tomato plants and begomovirus infection was confirmed by Polymerase chain reaction. The complete viral genome and associated betasatellites were amplified, cloned and sequenced from both corchorus and tomato samples. The genetic variability and phylogenetic relationships were determined for both isolates (corchorus and tomato). The complete genome sequences showed highest (99.5 % nt) similarity with tomato yellow leaf curl virus (TYLCV) and formed closest cluster with TYLCV-Tomato reported from Jizan and Al-Qasim, Saudi Arabia and betasatellites sequences showed highest similarity (99.8 % nt) with Tomato yellow leaf curl betasatellites-Jeddah followed by Tomato yellow leaf curl Oman betasatellites and formed closed cluster with TYLCV-Tomato. On the basis of results obtained from whiteflies transmission, sequence similarity and phylogenetic relationships; it is concluded that the identified virus could be a variant of TYLCV circulating in the Kingdom. The significance of this study demonstrated that the corchorus is serving as reservoir and alternative host and playing an important role in spreading the begomovirus associated disease in the Kingdom of Saudi Arabia.

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Acknowledgments

Author would like to thank General directorate of research grants (GDRG), King Abdulaziz City for science and technology (KACST-Riyadh) for providing large grant, bearing number: AT-66-34. Author would also like to gratefully acknowledge the research facility provided by Special Infectious Agents Unit, King Fahd Medical Research Center (KFMRC), King Abdulaziz University, Jeddah, Saudi Arabia.

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  1. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Post Box No-80216, Jeddah, 21589, Saudi Arabia

    Sayed Sartaj Sohrab

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Correspondence to Sayed Sartaj Sohrab.

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Sohrab, S.S. The role of corchorus in spreading of tomato yellow leaf curl virus on tomato in Jeddah, Saudi Arabia. VirusDis. 27, 19–26 (2016). https://doi.org/10.1007/s13337-015-0292-6

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