Skip to main content
SpringerLink
Log in

Occurrence of iris yellow spot virus from onion crops in Egypt

  • Original Article
  • Published:
VirusDisease Aims and scope Submit manuscript

Abstract

Iris yellow spot virus (IYSV) is a infects onion bulb and seed crops in many countries including Egypt. Results of the mechanical inoculation reveled that, small chlorotic lesions and systemic necrosis were observed on both Nicotiana benthamiana and Datura stramonium after 10 days, while there were no symptoms were appeared on the onion plant. The viral biological transmission with Thrips tabaci was highly reported to be efficiently for virus transmitted. Our results confirmed the presentence of virus-like particles of a Tospovirus infected onion leaf using transmission electron microscopy. Both of sequence and phylogenetic analysis of N gene revealed that our viral isolate is IYSV with 95 % identity with reported Israel isolate. The sequence of N gene had three motifs: casein kinase II Phosphorylation site, N-myristoylation site and protein kinase C phosphorylation site. These motifs are involved in regulation, activity and stability of IYSV. To our knowledge, this is the first molecular characterization of IYSV in Egypt.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

References

  1. Hafez EE, Abdelkhalek AA, El-Morsi AA, El-Sbahaby OA. First report of iris yellow spot virus infection of garlic and Egyptian leek in Egypt. Plant Dis. 2011;96:594.

    Article  Google Scholar 

  2. Schwartz HF, Mohan SK, Havey MJ, Crowe FJ. Introduction. In: Schwartz HF, Mohan SK, editors. Compendium of onion and garlic diseases and pests. 2nd ed. St. Paul: American Phytopathological Society; 2006.

    Google Scholar 

  3. Pappu HR, Jones RA, Jain RK. Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Res. 2009;141:219–36.

    Article  CAS  PubMed  Google Scholar 

  4. Mandal B, Jain RK, Krishnareddy M, Krishna Kumar NK, Ravi KS, Pappu HR. Emerging problems of tospoviruses (Bunyaviridae) and their management in the Indian subcontinent. Plant Dis. 2012;96:468–79.

    Article  Google Scholar 

  5. Gent DH, Schwartz HF, Khosla R. Distribution and incidence of iris yellow spot virus in colorado and its relation to onion plant population and yield. Plant Dis. 2004;88:446–52.

    Article  Google Scholar 

  6. Gent DH, du Toit LJ, Fichtner SF, Mohan SK, Pappu HR, Schwartz HF. Iris yellow spot virus: an emerging threat to onion bulb and seed production. Plant Dis. 2006;90:1468–80.

    Article  Google Scholar 

  7. Pappu HR, Rosales IM, Druffel KL. Serological and molecular assays for rapid and sensitive detection of Iris yellow spot virus infection of bulb and seed onion crops. Plant Dis. 2008;92:588–94.

    Article  CAS  Google Scholar 

  8. Bag S, Pappu HR. Symptomatology of Iris yellow spot virus in selected indicator hosts. Plant Health Prog. 2009;. doi:10.1094/PHP-2009-0824-01-BR.

    Google Scholar 

  9. Pappu HR, duToit LJ, Schwartz HF, Mohan SK. Sequence diversity of the nucleoprotein gene of iris yellow spot virus (Genus Tospovirus, Family Bunyaviridae) isolates from the western region of the United States. Arch Virol. 2006;151:1015–23.

    Article  CAS  PubMed  Google Scholar 

  10. Mandal B, Pappu HR, Culbreath AK. Factors affecting mechanical transmission of tomato spotted wilt virus to peanut (Arachis hypogaea). Plant Dis. 2001;85:1259–63.

    Article  Google Scholar 

  11. Birithia R, Subramanian S, Pappu HR, Muthomi J, Narla RD. Analysis of iris yellow spot virus replication in vector and non-vector thrips species. Plant Pathol. 2013;. doi:10.1111/ppa.12057.

    Google Scholar 

  12. Orion D, Frank A. An electron microscopy study of cell wall lysis by Meloidogyne javanica gelatinous matrix. Rev Nematol. 1990;13:105–7.

    Google Scholar 

  13. FAO (Food and Agricultural Organization of the United Nations), 2012 statistics. http://faostat.fao.org (2012).

  14. Francki RIB, Fauquet CM, Knudson DL, Brown F. Classification and nomenclature of viruses. In: Fifth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York. 1991. pp. 186–199

  15. Van Regenmortel, MHV, Fauquet CM, Bishop DHL, Carstens EB, Ester MK, Leman SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle DJ, Wickner RB. Virus taxonomy. classification and nomenclature of viruses. In: Seventh Report of the International Committee on Taxonomy of Viruses. San Diego: Academic Press; 2000.

  16. Moyer JW. Tospoviruses (Bunyaviridae). In: Granoff A, Webster RG, editors. Encyclopedia of virology. San Diego: Academic Press; 1999. p. 1803–7.

    Chapter  Google Scholar 

  17. Prins M, Goldbach R. The emerging problem of Tospovirus infection and nonconventional methods of control. Trends Microbiol. 1998;6:31–5.

    Article  CAS  PubMed  Google Scholar 

  18. Cortês I, Livieratos J, Derks A, Peters D, Kormelink R. Molecular and serological characterization of iris yellow spot virus, a new and distinct tospovirus species. J Phytopathol. 1998;88:1276–82.

    Article  Google Scholar 

  19. Pozzer L, Bezerra IC, Kormelink R, Prins M, Peters D, Resende RdeO, de Avilla AC. Characterization of a tospovirus isolate of iris yellow spot virus associated with a disease in onion fields in Brazil. Plant Dis. 1999;83:345–50.

    Article  CAS  Google Scholar 

  20. Kritzman A, Beckleman H, Alexandrow S, Cohen J, Lampel M, Zeidan M, Raccah B, Gera A. Lisianthus leaf necrosis: a new disease of lisianthus caused by Iris yellow spot virus. Plant Dis. 2000;84(11):1185–9.

    Article  CAS  Google Scholar 

  21. Kritzman A, Lampel M, Raccah B, Gera A. Distribution and transmission of iris yellow spot virus. Plant Dis. 2001;85(8):838–42.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Plant Protection and Bimolecular Diagnosis Department, Arid Lands Cultivation Research Institute, City of Scientific Research and Technological Applications, Alexandria, Egypt

    E. E. Hafez & A. A. Abdelkhalek

  2. Botany Department, Faculty of Science, Mansoura University, Mansoura, Egypt

    A. A. El-Morsi & O. A. El-Shahaby

Authors
  1. E. E. Hafez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. A. El-Morsi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. A. El-Shahaby
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. A. Abdelkhalek
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. E. Hafez.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hafez, E.E., El-Morsi, A.A., El-Shahaby, O.A. et al. Occurrence of iris yellow spot virus from onion crops in Egypt. VirusDis. 25, 455–459 (2014). https://doi.org/10.1007/s13337-014-0235-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13337-014-0235-7

Keywords

Search

Navigation