Abstract
Grapevine canker caused by Botryosphaeriaceae is currently one of most important trunk diseases in the world. This study identified one of the pathogens-B. dothidea that causes grapevine canker in most grapevine growing areas of China and verified its pathogenicity and geographical distribution. In total, 320 grapevine canker sympotoms samples were collected from 17 provinces between 2009 and 2011, and 4261 isolates were isolated from diseased samples. Among these, 1172 isolates purified from 150 samples were identified as B. dothidea by morphological and molecular characterizations. Three representative isolates were used to determine both optimal temperature and optimal nutrition medium for growth and germination. Pathogenicity of B. dothidea was determined in 25 grapevine cultivars. Pathogenicity results showed that most of the main grapevine cultivars growing in China are susceptible to the pathogen.
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References
Arauz LF, Sutton TB (1990) Protectant and after-infection activity of fungicides against Botryosphaeria obtusa on apple. Plant Dis 74:1029–1034
Auger J, Esterio M, Ricke G, Pérez I (2004) Black dead arm and basal canker of Vitis vinifera cv. Red Globe caused by Botryosphaeria obtusa in Chile. Plant Dis 88:1286
Beckman TG, Pusey PL, Bertrand PF (2003) Impact of fungal gummosis on peach trees. HortScience 38:1141–1143
Brown-Rytlewski DE, McManus PS (2000) Virulence of Botryosphaeria dothidea and Botryosphaeria obtusa on apple and management of stem cankers with fungicides. Plant Dis 84:1031–1037
Castillo-Pando M, Somers A, Green CD, Priest M, Sriskanthades M (2001) Fungi associated with dieback of Semillon grapevines in the Hunter Valley of New South Wales. Australas Plant Pathol 30:59–63
Choueiri E, Jreijiri F, Chlela P, Louvet G, Lecomte P (2006) Occurrence of grapevine decline and first report of Black dead arm associated with Botryosphaeria obtusa in Lebanon. Plant Dis 90:115
El-Goorani MA, El-Meleigi MA (1972) Dieback of grapevine by Botryodiplodia theobromae Pat. in Egypt. Phytophatol Mediterr 11:210–211
Filho OP, Ribeiro IJA, Kuniyuki H (1995) Podridao do troncoda videira (Vitis rinifera) causada por Dothiorella sp. forma anamorfica de Botryosphaeria dothidca. Summa Phytopathologica 21:40–42
Holmes J, Rich AE (1969) The control of Black rot cankers on apple trees in New Hampshire. Plant Dis Report 53:315–318
Larignon P, Dubos B (2001) The villainy of black dead arm. Wines Vines 82:86–89
Latorre BA, Toledo MV (1984) Occurrence and relative susceptibility of apple cultivars to Botryosphaeria canker in Chile. Plant Dis 68:36–39
Leavitt GM, Munnecke DE (1987) The occurrence, distribution, and control of Botryodiplodia theobromae on grapes (Vitis vinifera) in California. (Abstr.). Phytopathology 77:1690
Lehoczky J (1974) Black dead arm disease of grapevine caused by Botryosphaeria stevensii infection. Acta Phytopathol Hung 9:319–327
Li XH, Yan JY, Kong FF, Qiao GH, Zhang ZW, Wang ZY (2010) Botryosphaeria dothidea causing canker of grapevine newly reported in China. Plant Pathol 59:1170
Luque J, Martos S, Phillips AJL (2005) Botryosphaeria viticola sp. nov. on grapevines: a new species with a Dothiorella anamorph. Mycologia 97:1111–1121
Ma Z, Boehm EWA, Luo Y, Michailides TJ (2001) Population structure of Botryosphaeria dothidea from pistachio and other hosts in California. Phytopathology 91:665–672
Maas JL, Uecker FA (1984) Botryosphaeria dothidea cane canker of thornless blackberry. Plant Dis 68:720–726
Michailides TJ (1991) Pathogenicity, distribution, sources of inoculum, and infection courts of Botryosphaeria dothidea on pistachio. Phytopathology 81:566–573
Miliholland RD (1991) Muscadine grapes: some important diseases and their control. Plant Dis 75:113–117
Parker KC, Sutton TB (1993) Susceptibility of apple fruit to Botryosphaeria dothidea and isolate variation. Plant Dis 77:385–389
Pearson RC, Goheen AC (eds) (1988) Compendium of grape diseases and insects. APS, St. Paul, MN
Phillips AJL (1998) Botryosphaeria dothidea and other fungi associated with excoriose and dieback of grapevines in Portugal. Phytopath Z 146:327–332
Phillips AJL (2002) Botryosphaeria species associated with diseases of grapevines in Portugal. Phytopathol Mediterr 41:3–18
Qiu Y, Savocchia S, Steel CC, Ash GJ (2008) Botryosphaeria dothidea associated with grapevine trunk disease in south-eastern Australia. Australas Plant Pathol 37:482–485
Rovesti L, Montermini A (1987) A grapevine decline caused by Spaheropsis malorum widespread in the province of Reg-gio-Emilia. Inf Fitopatol 37:1–59
Rumbos C (1987) Twig and branch dieback of walnut trees induced by Botryosphaeria ribis. Plant Pathol 36:602–605
Savocchia S, Steel CC, Stodart BJ, Somers A (2007) Pathogenicity of Botryosphaeria species isolated from declining grapevines in sub tropical regions of Eastern Australia. Vitis 46:27–32
Siebert JB (2001) Eutypa: The economic toll on vineyards. Wines Vines April: 50–56
Starkey TE, Hendrix FF (1980) Reduction of substrate colonization by Botryosphaeriaobtusa. Plant Dis 64:292–294
Taylor A, Hardy GE, Wood P, Burgess T (2005) Identification and pathogenicity of Botryosphaeria spp. associated with grapevine decline in Western Australia. Australas Plant Pathol 34:187–195
Urbez-Torres JR, Gubler WD (2009) Pathogenicity of Botryosphaeriaceae Species Isolated from Grapevine Cankers in California. Plant Dis 93:584–592
Úrbez-Torres JR, Leavitt GM, Guerrero JC, Guevara J, Striegler K, Allen A (2007a) Identification of fungal pathogens associated with grapevine cankers in the main grape-growing areas of the United States and Mexico. Phytopathol Mediterr 46:109–110
Úrbez-Torres JR, Luque J, Gubler WD (2007b) First report of Botryosphaeria iberica and B. viticola associated with grapevine decline in California. Plant Dis 91:772
Van Niekerk JM, Crous PW, Groenewald JZ, Fourie PH, Halleen F (2004) DNA phylogeny, morphology and pathogenicity of Botryosphaeria species on grapevines. Mycologia 96:781–798
White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, Inc., New York, pp 315–322
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The research was supported by the Earmarked Fund for Modern Agro-industry Technology Research System (reference no. nycytx-30).
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Yan, J., Xie, Y., Yao, S. et al. Characterization of Botryosphaeria dothidea, the causal agent of grapevine canker in China. Australasian Plant Pathol. 41, 351–357 (2012). https://doi.org/10.1007/s13313-012-0135-5
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DOI: https://doi.org/10.1007/s13313-012-0135-5