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DNA polymerase β mutations and survival of patients with esophageal squamous cell carcinoma in Linzhou City, China

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Tumor Biology

Abstract

Linzhou City in northern China has a high incidence of esophageal squamous cell carcinoma (ESCC). This study retrospectively analyzed the data of 231 cases with ESCC collected from 1998 to 2012. Mutations of DNA polymerase β (polβ) gene in the ESCC samples from patients in Linzhou City were examined by amplifying polβ cDNA by RT-PCR followed by cloning and sequencing. Mutations in polβ were found in 105 cases (45.9 %). Nine types of mutations were identified in the polβ cDNA; the most common were 177–234 nt deletion (11.3 %), 462 nt G → T (9.1 %), and 648 nt G → C (6.9 %). Mutations in polβ appeared to be associated with TNM status (P = 0.048). Follow-up data was used for survival analysis. The overall 5-year survival rate of the 231 patients was 37.4 %; the rate for patients with wild-type (WT) polβ was 41.8 %. Compared with the WT polβ group, the median survival for patients with specific mutations (177–234 nt deletion, 462 nt G → T, or 613 nt A → T) was significantly shorter (all P = 0.000), and the 5-year survival rate decreased to 0 %. Patients with the 648 nt G → C mutation had improved survival (P = 0.000) with a 5-year survival rate of 100 %. Our results identified nine types of mutations within polβ cDNA in ESCC patients with four mutations related to patient survival.

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Acknowledgments

This study was supported by a grant from the National Natural Science Foundation of China (no. 81272188).

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Authors and Affiliations

  1. College of Basic Medical Sciences, Zhengzhou University, No. 100, Kexue Road, Zhengzhou, 450001, Henan Province, China

    Min Li, Wenqiao Zang, Yuanyuan Wang, Xiaoyan Xuan, Jimin Zhao, Lulu Liu, Ziming Dong & Guoqiang Zhao

  2. Department of Immunology and Microbiology, Henan Medical College for Stuff and Workers, Zhengzhou, 451191, Henan Province, China

    Yunyun Ma

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  1. Min Li
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  2. Wenqiao Zang
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  3. Yuanyuan Wang
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  9. Guoqiang Zhao
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Correspondence to Guoqiang Zhao.

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Li, M., Zang, W., Wang, Y. et al. DNA polymerase β mutations and survival of patients with esophageal squamous cell carcinoma in Linzhou City, China. Tumor Biol. 35, 553–559 (2014). https://doi.org/10.1007/s13277-013-1077-4

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  • DOI: https://doi.org/10.1007/s13277-013-1077-4

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