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Circular RNA circ_0024037 suppresses high glucose-induced lens epithelial cell injury by targeting the miR-199a-5p/TP53INP1 axis

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Abstract

Background

Diabetic cataract is a common ocular complication of diabetes. Circular RNA (circRNA) can participate in a variety of regulatory processes of a variety of eye diseases, including diabetic cataract.

Objective

Nowadays, the biological mechanism underlying circ_0024037 during diabetic cataract is not completely understood. This study was designed to explore the biological role of circ_0024037 in high glucose (HG)-induced lens epithelial damage.

Result

Circ_0024037 and TP53INP1 were significantly up-regulated while miR-199a-5p was significantly down-regulated in the diabetic cataract tissues and HG-induced human lens epithelial cells (HLECs). Knockdown of circ_0024037 significantly promoted the HG-induced HLECs cell proliferation, inhibited apoptosis, decreased MDA level as well as increased GSH-PX level. The dual-luciferase reporter assay and RIP assay showed that circ_0024037 served as a sponge of miR-199a-5p and miR-199a-5p could directly target TP53INP1 in HLECs.

Conclusion

Circ_0024037 knockdown protected HLECs from the HG-induced dysfunction by regulating the miR-199a-5p/TP53INP1 pathway in diabetic cataract. Our findings provid novel insights into the pathogenesis of diabetic cataract.

Highlights

  • Circ_0024037 is up-regulated in diabetic cataract;

  • Circ_0024037 regulates proliferation, apoptosis, MDA, and GSH-PX level in high- glucose-induced HLECs;

  • Circ_0024037/miR-199a-5p/TP53INP1 involves in the progression of diabetic cataract.

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Author notes

  1. Liping Zhou and Yanhua Zheng contributed equally to this work.

Authors and Affiliations

  1. Department of Ophthalmology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, No. 136 Jingzhou Street, Xiangcheng District, Xiangyang, 441000, Hubei, China

    Liping Zhou, Yue Xu & Pincheng Shen

  2. Aier Eye Hospital of Wuhan University (Wuhan Aier Eye Hospital), 481 Zhongshan Road, Wuchang District, Wuhan, 430061, Hubei, China

    Yanhua Zheng

Authors
  1. Liping Zhou
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  2. Yanhua Zheng
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  3. Yue Xu
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  4. Pincheng Shen
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Contributions

All authors have been involved in the management of the patient and in the conception of the manuscript. The specific authors’ contribution is listed below. ZL, ZY: Methodology, formal analysis and investigation, writing-original draft. XY: Formal analysis and investigation, writing—review and editing. SP: Conceptualization, formal analysis and investigation, resources.

Corresponding authors

Correspondence to Yue Xu or Pincheng Shen.

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Conflict of interest

Zhou LP declares that she has no conflict of interest. Zheng YH declares that she has no conflict of interest. Xu Y declares that she has no conflict of interest. Shen PC declares that she has no conflict of interest.

Ethical approval

This study was permitted by the Ethics Committee of Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science.

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Zhou, L., Zheng, Y., Xu, Y. et al. Circular RNA circ_0024037 suppresses high glucose-induced lens epithelial cell injury by targeting the miR-199a-5p/TP53INP1 axis. Mol. Cell. Toxicol. 20, 281–291 (2024). https://doi.org/10.1007/s13273-023-00340-7

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  • DOI: https://doi.org/10.1007/s13273-023-00340-7

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