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PCDH8 protects MPP+-induced neuronal injury in SH-SY5Y cells by inhibiting MAPK pathway

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Abstract

Background

Parkinson's disease (PD) is a common degenerative disease of the nervous system in the elderly.

Objectives

To investigate the effect of protocadherin 8 (PCDH8) on PD and explore the underlying mechanism.

Results

PCDH8 was down-regulated in PD. Silencing PCDH8 inhibited cell viability, increased cell cytotoxicity and apoptosis, but overexpression of PCDH8 acted the opposed effects. Then, silencing PCDH8 increased the levels of IL-1β, IL-8, TNF-α and ROS production, but decreased SOD and CAT activity, while up-regulation of PCDH8 acted the opposed effects. In addition, down-regulation of PCDH8 activated MAPK pathway, but up-regulation of PCDH8 inhibited MAPK pathway. Furthermore, in SH-SY5Y cells treated by MPP+, SB203580 reversed cell injuries induced by silencing PCDH8.

Conclusion

PCDH8 was down-regulated in PD, and PCDH8 protected MPP+-induced neuronal injury in SH-SY5Y cells via inhibiting MAPK pathway.

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Authors and Affiliations

  1. Department of Neurology, Jiyang District People’s Hospital of Jinan, Jinan, 251400, Shandong, People’s Republic of China

    Huaming Wei

  2. Department of Infection Management, Chiping District People’s Hospital, Liaocheng, 252100, People’s Republic of China

    Yuping Wu

  3. Department of Neurology, Gaotang County People’s Hospital, Liaocheng, 252800, Shandong, People’s Republic of China

    Huanxia Zhuang

  4. Department of Neurology, People’s Hospital of Leling City, No.38 Anju Road, Leling, 253600, Shandong, People’s Republic of China

    Hongyi Su

Authors
  1. Huaming Wei
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  2. Yuping Wu
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  3. Huanxia Zhuang
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  4. Hongyi Su
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Contributions

Huaming Wei designed the study and wrote the paper; Huaming Wei and Yuping Wu provided technical support and obtained the data; Hongyi Su and Huanxia Zhuang analyzed data; Final approval of the version to be published: All authors.

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Correspondence to Hongyi Su.

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Huaming Wei declares that he has no conflict of interest. Yuping Wu Wei declares that he has no conflict of interest. Huanxia Zhuang declares that she has no conflict of interest. Hongyi Su declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Wei, H., Wu, Y., Zhuang, H. et al. PCDH8 protects MPP+-induced neuronal injury in SH-SY5Y cells by inhibiting MAPK pathway. Mol. Cell. Toxicol. 19, 293–302 (2023). https://doi.org/10.1007/s13273-022-00257-7

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  • DOI: https://doi.org/10.1007/s13273-022-00257-7

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