Abstract
Background
Coal dust exposure has caused a variety of lung diseases. In addition to genotoxicity and cytotoxicity, other biological changes caused by coal dust (CD) exposure need further study.
Objective
To observe the cellular transformation effects of CD exposure and explore its underlying molecular mechanism, human bronchial epithelial cells (BEAS-2B) were cultured with continuous CD exposure. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, colony formation assay, wound healing assay, next-generation sequencing (NGS) and western blotting were performed to observe the cell proliferation, migration, genomic transcription and pathological signaling pathways.
Results
We demonstrated that BEAS-2B cells with long-term chronic CD exposure show accelerated proliferation rate and enhanced migration ability, and have altered gene expression profiles and aberrant activation of EGFR/Raf/ERK and PI3K/AKT/mTOR pathways.
Conclusions
The results indicate that chronic CD exposure could induce abnormal proliferation and migration of BEAS-2B cells, lead to the transformation potential of human bronchial epithelial cells.
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Acknowledgements
The National Natural Science Foundation of China (NO. 82071862, 81872017, 81572431), Anhui Provincial Science and Technology program (NO. 1604a0802094, 202004j07020053), University Natural Science Research Project of Anhui Province (NO. KJ2018ZD011, KJ2018A0097, KJ2019A0093, KJ2020A0340, KJ2020A0338) and Research Foundation of the Institute of Environment-friendly Materials and Occupational Health (Wuhu), Anhui University of Science and Technology (ALW2020YF11) funded this research.
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XT and AL: conceived and designed the study; AL, YZ and RW: performed data analysis; RX, YM, LS and WC: performed the biological methodology and acquisition of data; AL: drafted the manuscript (assign co-first authors order according to workload). All the authors read and approved the final version of the manuscript.
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Amin Li declares that she has no conflict of interest. Yinci Zhang declares that she has no conflict of interest. Ruikai Wang declares that he has no conflict of interest. Ruyue Xu declares that she has no conflict of interest. Yongfang Ma declares that she has no conflict of interest. Li Song declares that he has no conflict of interest. Weiya Cao declares that she has no conflict of interest. XiaolongTang declares that he has no conflict of interest.
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Li, A., Zhang, Y., Wang, R. et al. Coal dust exposure induces proliferation and migration of human bronchial epithelial cells. Mol. Cell. Toxicol. 19, 237–245 (2023). https://doi.org/10.1007/s13273-022-00252-y
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DOI: https://doi.org/10.1007/s13273-022-00252-y