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Transcriptomic profiling of soybean in response to UV-B and Xanthomonas axonopodis treatment reveals shared gene components in stress defense pathways

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Abstract

Soybean (Glycine max [L.]) has evolved to survive under abiotic and biotic stress conditions by utilizing multiple signaling pathways. Although several studies have revealed shared defense signaling pathways in plants, the majority of components at the convergence points of signaling pathways triggered by both abiotic and biotic stress remain poorly understood. Here, we profiled the overall transcriptional responses of soybean to two different types of stress using the UV-B-resistant cultivar, Buseok, and the UV-B-sensitive cultivar, Cheongja 3, as well as two near isogenic lines carrying bacterial leaf pustule (BLP) disease-resistant and -susceptible alleles. We compared transcript abundance and identified genes that commonly respond to UV-B stress and BLP disease. In addition, we surveyed the co-localization of differentially expressed genes (DEGs) and their paralogs with abiotic and biotic stress-related quantitative trait loci (QTLs) on the soybean genome. Among 14 DEGs that respond to both stresses, five DEGs are involved in the jasmonic acid (JA) metabolic pathway, encoding Jasmonate ZIM (Zinc-finger protein expressed in Inflorescence Meristem) domain-containing protein 1 (JAZ 1), a negative regulator of JA signaling. Two DEGs for JAZ 1 were co-localized with biotic stress-related QTLs. One DEG encoding the stress-induced protein starvation-associated message 22 and its two paralogs were co-localized with both abiotic and biotic stress-related QTLs. The results of this study help elucidate general responses to abiotic and biotic stress in soybean, thereby helping breeders improve stress-resistant soybean cultivars.

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Acknowledgements

This work was supported by a Grant from the Next Generation BioGreen 21 Program (Code No. PJ01102701), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Plant Science and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea

    Min Young Yoon, Moon Young Kim, Jayern Lee, Taeyoung Lee, Jungmin Ha & Suk-Ha Lee

  2. Plant Genomics and Breeding Institute, Seoul National University, Seoul, Republic of Korea

    Moon Young Kim, Jungmin Ha & Suk-Ha Lee

  3. Experiment & Analysis Division, Yeongnam-Regional Office, Animal and Plant Quarantine Agency, Busan, Republic of Korea

    Kil Hyun Kim

  4. Department of Crop Science and Biotechnology, Dankook University, Cheonan, Republic of Korea

    Yong Hwan Kim

  5. Crop Genomics Lab, Seoul National University, Rm. 4105 Bldg. 200 CALS, 1 Gwanak-ro, Gwanak-Gu, Seoul, 08826, Republic of Korea

    Suk-Ha Lee

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  1. Min Young Yoon
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Correspondence to Suk-Ha Lee.

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Min Young Yoon declare that she have no conflict of interest. Moon Young Kim declare that she have no conflict of interest. Jayern Lee declare that he have no conflict of interest. Taeyoung Lee declare that he have no conflict of interest. Kil Hyun Kim declare that he have no conflict of interest. Jungmin Ha declare that he have no conflict of interest. Yong Hwan Kim declare that he have no conflict of interest. Suk-Ha Lee declare that he have no conflict of interest.

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Yoon, M.Y., Kim, M.Y., Lee, J. et al. Transcriptomic profiling of soybean in response to UV-B and Xanthomonas axonopodis treatment reveals shared gene components in stress defense pathways. Genes Genom 39, 225–236 (2017). https://doi.org/10.1007/s13258-016-0490-7

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  • DOI: https://doi.org/10.1007/s13258-016-0490-7

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