Cataract-causing mutation S228P promotes βB1-crystallin aggregation and degradation by separating two interacting loops in C-terminal domain

β/γ-Crystallins are predominant structural proteins in the cytoplasm of lens fiber cells and share a similar fold composing of four Greek-key motifs divided into two domains. Numerous cataract-causing mutations have been identified in various β/γ-crystallins, but the mechanisms underlying cataract caused by most mutations remains uncharacterized. The S228P mutation in βB1-crystallin has been linked to autosomal dominant congenital nuclear cataract. Here we found that the S228P mutant was prone to aggregate and degrade in both of the human and E. coli cells. The intracellular S228P aggregates could be redissolved by lanosterol. The S228P mutation modified the refolding pathway of βB1-crystallin by affecting the formation of the dimeric intermediate but not the monomeric intermediate. Compared with native βB1-crystallin, the refolded S228P protein had less packed structures, unquenched Trp fluorophores and increased hydrophobic exposure. The refolded S228P protein was prone to aggregate at the physiological temperature and decreased the protective effect of βB1-crystallin on βA3-crystallin. Molecular dynamic simulation studies indicated that the mutation decreased the subunit binding energy and modified the distribution of surface electrostatic potentials. More importantly, the mutation separated two interacting loops in the C-terminal domain, which shielded the hydrophobic core from solvent in native βB1-crystallin. These two interacting loops are highly conserved in both of the N- and C-terminal domains of all β/γ-crystallins. We propose that these two interacting loops play an important role in the folding and structural stability of β/γ-crystallin domains by protecting the hydrophobic core from solvent access. Electronic supplementary material The online version of this article (doi:10.1007/s13238-016-0284-3) contains supplementary material, which is available to authorized users.


INTRODUCTION
The vertebrate lens is a delicate machine with the ability to transmit and focus visible lights on the retina. The optical functions of the lens are achieved by the three-dimensional packing of the highly differentiated lens fiber cells and the unique protein expression profile in lens cells (Benedek, 1971;Bloemendal et al., 2004). In mature lens fiber cells, all organelles have been degraded via an apoptic-like pathway to avoid light scattering (Bassnett, 2009). The lack of protein turnover machineries implies that the lens proteins are required to maintain their solubility and stability for several decades (Bloemendal et al., 2004). Once the lens proteins precipitate into large aggregates, the transmission of visible lights will be scattered, which will lead to cataract (Benedek, 1997). Cataract, a typical protein aggregation disease, is one of the leading causes of human blindness worldwide. Similar to the other protein aggregation diseases, there are no clinical drugs available for cataract prevention and treatment. Currently surgical treatment is the only available method to cure both congenital and aged cataracts. Very recently, the findings of crystallin aggregation reversed by lanosterol  and regeneration of human lens using endogenous stem cells of infants (Lin et al., 2016) provide new insights in the developing of novel strategies for cataract prevention and treatment.
Each polypeptide of β/γ-crystallins is composed of four Greek-key motifs divided into two domains, the N-and C-terminal domain (NTD and CTD, respectively) (Bax et al., 1990). The hydrophobic core of each domain is formed by two Greek-key motifs and therefore the integrity of these Greek-key motifs is important to the structure and stability of β/γ-crystallins. Mutations of key residues involved in Greekkey motif formation in γD have been associated with more severe cataracts than those occurred at the loops, termini or surfaces of β/γ-crystallins (Vendra et al., 2013). Frameshift or nonsense mutations in β/γ-crystallins usually result in the loss of protein segments and thereby block the hydrophobic core formation. Besides acted as Greek-key motif breakers, mutations in β-crystallins may lead to cataract via complicated mechanisms such as changes in oligomerization and protein-protein interactions (Zhang et al., 2014;Xi et al., 2014a, b;Xu et al., 2010;Wang et al., 2013;Xu et al., 2012;Liu and Liang, 2005;Serebryany and King, 2014). Among the cataract-causing mutations in β-crystallins, there is a number of missense mutations occurred at the last β-strand of Greek-key motif 4. The last β-strand at the C-terminus of β-crystallins not only participates in the formation of the hydrophobic core, but also contributes to subunit interactions. Thus mutations occurred at the last β-strand may lead to cataract via quite different mechanisms. For example, V187E in βB2 is a Greek-key motif breaker, V187M in βB2 affects the hydrophobic core, while R188H in βB2 and R233H in βB1 affect the dynamic oligomeric equilibrium (Zhang et al., 2014;Xi et al., 2014a). At present it is difficult to predict how ADCC is caused by mutations in β-crystallins and experimental investigations are still necessary to elucidate the deleterious effects of these mutations.
In this research, we studied the possible mechanism of ADCC caused by the S228P mutation in βB1. In 2007, S228P in βB1 was identified as a causive mutation of ADCC in a four-generation large Chinese family with nuclear cataract phenotype (Wang et al., 2007). S228 is fully conserved among various human β/γ-crystallins, but can be substituted by Ala in βB1 from the other vertebrate species (Fig. S1A). Structurally, S228 is the first residue of the last β-strand in Greek-key motif 4 and does not participate into the formation of monomer-monomer or dimer-dimer interfaces (Fig. S1B) (Montfort et al., 2003). Ser is a polar residue and thereby S228 is not a key residue of the hydrophobic core. To elucidate how βB1 structure and stability are affected by the S228P mutation, we compared the behaviors of the wild type (WT) βB1 and the S228P mutant at the protein and cellular levels. Our results showed that S228 stabilized two interacting loops to shield the CTD hydrophobic core from solvent access. The S228P mutation separated the two interacting loops from each other and allowed water molecules to penetrate into the hydrophobic core. More importantly, the positions and interaction of these two loops are highly conserved in various β/γ-crystallins, suggesting that the shielding effect of these two loops is important to the folding and stability of β/γ-crystallin domains. Cells with aggregates (%) β B 11 -1 4 2 β B 11 -1 7 3 β B 11 -2 2 9 S 2 2 8 P W T Figure 1. The S228P mutation promoted βB1 aggregation and degradation in human HEK 293T cells and E. coli BL21 cells. (A) Representative confocal images of HEK 293T cells with exogenously expressed WT βB1 and S228P fused by EGFP at the N-terminus. The empty vector of pEGFP-C3 was also transfected and used as the control. The βB1 proteins were visualized by the tagged GFP (green). The aggresomes were recognized by the marker protein p62 (red). The nucleus was stained by Hoechst 33342 (blue). The white arrows indicate the typical aggregates formed by GFP-S228P, which did not colocalize with p62. (B) Representative confocal images of HEK 293T cells expressing WT βB1 and S228P fused by EGFP at the C-terminus. The white arrows indicate the typical p62-positive aggregates formed by S228P-GFP. (C) Quantitative analysis of protein aggregation in HEK 293T cells. The percentages were obtained by calculating the ratios of cells with aggregates in ten random viewing field and the presented data were from three independent experiments. (D) Western blot analysis of the exogenously expressed WT βB1 and S228P in the HEK 293T cells. Actin was used as the loading control. The black arrows indicate the appearance of protein degradation. (E) SDS-PAGE and Western blot analysis of the WT βB1 and S228P overexpressed in E. coli BL21 cells. The proteins were purified by Ni-column and Superdex column. The degraded segments were identified by mass spectrometry. The Western blot analysis was performed using antibody against His-tag. (F) The proteolytic fragment βB1 1-229 was prone to form aggregates in HEK 293T cells, while βB1 1-142 and βB1 1-173 were not. Representative confocal images are shown in Fig. S6. S228P promotes βB1-crystallin aggregation and degradation RESEARCH ARTICLE HEK 293T cells ( Fig. 1A and 1B). The position of the GFP tag does not affect the cellular distribution of βB1. The S228P mutant was prone to form intracellular aggregates for both types of fused proteins, while cells expressing GFP-S228P had a larger portion of cells containing aggregates than cells expressing S228P-GFP (Fig. 1C). The difference in percentages of cells with aggregates was not caused by the expression levels of the two types of fused proteins (Fig. S2). It is worth noting that GFP-WT also had a larger percentage of cells with aggregates than WT-GFP. A possible explanation is that the N-terminal extension has been shown to assist the folding of βB1  and thereby the N-terminal tagged GFP might interfere with the function of the N-terminal extension. The major difference between the two types of fused proteins was their colocalization with p62, an aggresome marker. The aggregates formed by S228P-GFP were p62-positive, while those by GFP-S228P were p62-negative. Most crystallin mutants formed p62-positive aggresomes in human cells Xi et al., 2015). We further confirmed that GFP-S228P formed p62negative aggregates by fusing S228P by a Flag tag at the N-terminus (Fig. S3). The absolute number of percentage of cells with Flag-S228P aggregates was lower than that of GFP-S228P, which might be caused the by lower expression level of Flag-S228P in the cells or inaccessibility of the Flag tag buried in the aggregates. Nonetheless, the results showed that the formation of p62-negative aggregates was independent on the type of tag fused at the N-terminus of S228P. Previously, the p62-positive crystallin aggregates have been shown to induce cell death. Here we found that the p62-negative S228P aggregates could also be toxic to the cells and induce cell apoptosis and necrosis (Fig. S3).
The exogenously expressed proteins were analyzed by Western blot using antibody against GFP (Fig. 1D). Consistent with the results from confocal microscope observations, Western blot analysis indicated that the WT proteins were predominantly in the supernatant fraction of cell lysates, while the mutated proteins were in both of the supernatant and precipitation fractions. There were S228P proteolytic segments that could be pulled down by the GFP antibody, while no proteolysis was observed for the WT proteins. GFP-S228P was more susceptible to degradation than S228P-GFP, which might be caused by the protective effect of the tagged GFP at the C-terminus. The proteolysisprone GFP-S228P had higher propensity to form intracellular aggregates (Fig. 1C), suggesting that proteolysis might increase the aggregatory propensity of the protein.
The N-terminal His-tagged WT βB1 could be successfully obtained from the supernatant fractions of E. coli cells cultivated at 37°C (Fig. S4A). However, most of the S228P mutated proteins existed in the inclusion bodies when using the same induction and cultivating conditions as those for the expression of WT protein. When the E. coli cells were cultivated at 12°C, a small fraction of recombinant S228P proteins was in the soluble fractions and could be purified by Nicolumn (Fig. S4B). However, little S228P proteins with the predicted molecular size could be obtained after purification by gel filtration chromatography at 4°C even though there were no halts during purification (Figs. 1E and S4B). Western blot analysis using antibody against His indicated that the purified proteins were His-tagged recombinant proteins (Fig. 1E). Mass spectrometry analysis of the major bands separated by SDS-PAGE indicated that the purified soluble proteins were N-terminal proteolytic segments of βB1, while the proteins in the inclusion bodies were dominated by intact S228P. These observations indicated that the behaviors of the S228P mutant in the E. coli cells were similar to those in HEK 293T cells, implying that the high susceptibility to aggregation and degradation was an intrinsic property of the S228P mutated βB1. To study whether proteolysis affect S228P aggregation, three N-terminal fragments, βB1 1-142 , βB1 1-173 and βB1 1-229 were constructed and overexpressed in the E. coli cells. Part of the recombinant βB1 1-142 and βB1 1-173 proteins existed in the supernatant fractions, while most of βB1 1-229 proteins were in the inclusion bodies ( Fig. S5). In the HEK 293T cells, most cells expressing βB1 1-142 and βB1 1-173 did not contain protein aggregates, while about 45% cells expressing βB1 1-229 with a GFP tag at the N-terminus contained p62-negative aggregates (Figs. 1F and S6). This suggested that βB1 1-229 was the main aggregation-prone proteolytic fragment.
Recently, it has been identified that lanosterol, a sterol intermediate in the cholesterol synthetic pathway, can reverse crystallin aggregation . The HEK 293T cells containing exogenously expressed WT and mutated βB1 were treated by various concentrations of lanosterol or cholesterol. The results herein confirmed the previous finding that lanosterol but not cholesterol could decrease the percentage of cells with crystallin aggregates in a concentration-dependent manner (Fig. 2). Lanosterol was effective to redissolve preformed aggregates for both types of fused proteins, implying that the action of lanosterol was independent on the nature of crystallin aggregates, p62positive or p62-negative.

The S228P mutation modifies βB1 refolding pathway
The results in Fig. 1 suggested that the S228P mutation might affect the folding of βB1 in human and E. coli cells. Equilibrium refolding of the GdnHCl-denatured proteins was performed to verify this proposal (Fig. 3). Similar to previous observations , the refolding of βB1 was a multi-state process with the appearance of at least two equilibrium refolding intermediates (2U → 2I 2 → I 1 → N 2 ). As proposed previously, I 2 at 2.2 mol/L GdnHCl is a partially folded monomeric intermediate, while I 1 at ∼1.3 mol/L is a dimeric intermediate. I 1 may be a state with extra shielding of the Trp fluorescence as evidenced by the blue-shift of the maximum emission wavelength (E max ) or larger Parameter A value when compared to the native state . Thus the transition from I 1 to N involves structural arrangements around the Trp fluorophores. The transition curves from Trp fluorescence intensity, ANS fluorescence and turbidity were almost superimposed for the WT and S228P proteins. As for the transition curves from CD, E max and Parameter A, no significant difference was observed for the transition from U to I 2 , while large deviations between the two proteins were found for the I 2 → I 1 transition. I 1 of S228P had different structural features from those of WT βB1. Compared with I 1 of WT βB1, I 1 of S228P had less regular secondary structures, a red-shift of E max and a smaller Parameter A value, suggesting that the S228P mutation retarded the formation of a well-packed dimeric intermediate.

The refolded S228P mutant is unstable and prone to aggregate
The S228P mutation probably affected the final step of βB1 dimer assembly and structural rearrangements (Fig. 3). Since the native state of S228P could not be obtained by purification of recombinant proteins, we purified the inclusion bodies from the E. coli cells and the proteins in the waterinsoluble fraction were dissolved by buffer A containing 6 mol/L GdnHCl. The refolded S228P was successfully obtained by one-step dilution of the 6 mol/L GdnHCl denatured proteins in the refolding buffer (buffer A). The refolded S228P was prone to degrade and aggregate and thus fresh refolded samples were used for the biophysical measurements (Fig. 4). Compared with the WT protein, S228P possessed more disordered structures as revealed by the shape of far-UV CD spectra (Fig. 4A). The E max of S228P was similar to that of the WT protein (Fig. 4B). However, S228P had a much larger Trp fluorescence intensity, implying that the mutation impaired the correct quenching of Trp fluorophores in native β/γ-crystallins (Chen et al., 2009;Chen et al., 2006;Kosinski-Collins et al., 2004). The refolded S228P had much larger ANS fluorescence than the WT βB1 (Fig. 4C), suggesting that the refolded S228P failed to bury the hydrophobic side chains in the interior of the molecule. The oligomeric status of refolded S228P could not obtained by static light scattering due to fast degradation and aggregation of the proteins. Thus the molecular size of refolded S228P was qualitatively evaluated by resonance Raleigh light scattering, which is a sensitive monitor of change in molecular size of soluble oligomers (He et al., 2009). The refolded S228P protein had a much higher light scattering intensity than the WT protein (Fig. 4D), implying that the refolded S228P either had more disordered regions or had a larger oligomeric size.
In the lens, β-crystallins are proposed to form homomers or heteromers (Slingsby and Bateman, 1990). In the βA/βB heteromers, βB-crystallins are thought to protect the relative instable βA-crystallins against aggregation Wang et al., 2011a, b;Bateman et al., 2001;Bateman et al., 2003). The heteromers were formed by mixing equal molar of homomers and incubated at 37°C as described previously . SEC (Fig. 4E) and SDS-PAGE analysis (Fig. 4F) were used to characterize heteromer formation. As the control, the WT βB1 and βA3 prefer to exist as heterodimer after incubation. However, almost all molecules of refolded S228P precipitated and little βA3/ S228P heteromers could be obtained. To elucidate whether refolded S228P lost its ability to associate with βA3 or aggregated before association, we monitored the timecourse change in turbidity after mixing βA3 and S228P at 37°C. As the controls, βA3, βB1 and βA3/βB1 were stable at the physiological temperature. However, refolded S228P aggregated rapidly at 37°C (Fig. 4G). The addition of βA3 10 μm 10 μm 10 μm 10 μm 10 μm 10 μm S228P promotes βB1-crystallin aggregation and degradation

RESEARCH ARTICLE
could elongate the lag time and slow down the rate of S228P aggregation, but did not decrease the extent of aggregation (Fig. S7). Thus it seems that the association between βA3 and S228P could occur but was greatly weakened by the mutation. To confirm this proposal, the 6 mol/L GdnHCl-denatured S228P were refolded in the presence or absence of βA3 ( Fig. 4H and 4I). Similar to previous results Wang et al., 2011a), the WT βB1 could associate with βA3 during co-refolding and protect βA3 against misfolding and aggregation. The S228P mutant did not aggregate during refolding from the GdnHCl-denatured state, but partially lost the protective effect on βA3. Thus the S228P mutation weakened the ability of βB1 to form heteromers with βA3, probably by perturbation of the correct refolding of βB1.
The S228P mutation separates two interacting loops that shield the hydrophobic core of the C-terminal domain from solvent S228 is the first residue forming the last β-strand of Greekkey motif 4 in βB1 (Fig. S1B). Ser has a polar side chain and consequently S228P is not a key residue in the formation of the hydrophobic core. MD simulations were performed for both the dimeric and monomeric (subunit A of the dimer) structures to explore the structural basis for the deleterious effects of the S228P mutation on βB1 structure, folding, assembly and stability. The structural variations for all simulated proteins were within 3 Å and the simulations could reach equilibrium after ∼7.5 ns (Fig. S8A). Under our simulating conditions, no significant difference was observed between the simulated dimeric and monomeric structures (Fig. S8B). Alignment of the dimeric structures of βB1 and S228P indicated that the mutation did not alter the overall fold of βB1 (Fig. 5A). However, the S228P mutation greatly reduced the subunit binding energy arisen from electrostatic interactions (Figs. 5B and S9A). It has been proposed that the subunit interface is mainly stabilized by the H-bonding network among two acidic residues (D169 and D170) and two basic residues (R231 and R233) in each subunit (Montfort et al., 2003). The H-bonding network was maintained in the subunit interface of S228P, but the positions of these charged residues were slightly altered in the simulated S228P dimeric structure (Fig. S9B). A close inspection of the surface electrostatic potentials indicated that the S228P mutation altered the distribution of charged/polar residues around the subunit interface (Fig. 5C). The mutation did not significantly alter the solvent accessible surface area (SAA) of the subunit, but increased the SAA of the dimer (Fig. 5D), which further suggested that decrease in subunit binding   energy might result in the exposure of more buried residues in the interface to solvent.
Although a significant increase in ANS fluorescence was observed for the refolded S228P protein (Fig. 4C), it seems that the overall hydrophobic exposure was not significantly affected by the S228P mutation when analyzing the surface electrostatic potentials of the simulated dimeric and monomeric structures (Figs. 5C and S10). The main difference between the WT and mutated proteins was the distribution of surface charged/polar regions and the hydrophobic regions. Thus a possible explanation for the increased ANS fluorescence shown in Fig. 4C is that the mutated protein might contain more hydrophobic sites accessible by the ANS probes. A comparison of the spatial aggregation propensity (SAP) values (Chennamsetty et al., 2009) indicated that the major difference between the two proteins was at the region, 199 PGYRG 203 (Fig. 5E). The positions of most Trp residues except for W237 at the C-terminal extension were not significantly affected by the mutation (Fig. 5F), which is consistent with the unchanged E max of Trp fluorescence (Fig. 4B). The mutation-induced increase of Trp fluorescence was more likely to be caused by the change in the rigidness of the hydrophobic core or quenching of Trp fluorescence by adjacent residues.
The major structural changes in the subunit structure caused by the S228P mutation was obtained by tracing the structural fluctuations during the 20 ns simulations (Fig. S11A). Two adjacent interacting loops, P199-G203 and P172-V176, had large variations in S228P but not in WT βB1 (Fig. S11B). Similar results could also be obtained by comparing the RMSD values of the final frame of the simulated structures (Fig. 6A) except for the identification of an additional region ranging from A84 to R90 in NTD, which is equivalent to the G168 to G178 region in CTD ( Fig. 6B and  6C). It is worth noting that D169 is a key residue forming subunit interface (Figs. 5B and S9B) (Montfort et al., 2003). Structurally, most parts of these three regions with the largest structural changes formed loops and surface helices to cover the hydrophobic core of NTD and CTD. The movement of the A84 to R90 region did not affect the positions of the two interacting loops in NTD, whereas the closely packed loops in CTD were separated from each other in the simulated structures of S228P (Fig. 6D). The distances between the two interacting loops provided a quantitative evaluation of separation (Fig. 6E). The distance between the two loops was about 6.2 Å in both NTD and CTD of the WT βB1 as well as NTD of S228P, but separated by 9.3 Å in CTD of S228P. The separation of the two interacting loops by the S228P mutation allowed the solvent molecules to penetrate in the hydrophobic core of CTD (Fig. 6F) and thereby might retard CTD dehydration during refolding. In WT βB1, S228 helps to tether the P199-Y201 loop by forming H-bonds with Y196, Y198 and Y201 via its side-chain OH and backbone NH, which were disrupted by the S228P mutation (Fig. 6G). The above structural analysis suggested that the S228P mutation did not perturb the hydrophobic core directly, but influenced the protection of the hydrophobic core from solvent by the two interacting loops.

DISCUSSION
Cataract is a prevalent disease that is directly caused by the appearance of light-scattering crystallin aggregates (Benedek, 1997;Moreau and King, 2012). Although crystallin aggregation is a common feature of various cataracts, the ways of highly soluble crystallins to aggregation may be very complicated (Vendra et al., 2013;Zhang et al., 2014;Xi et al., 2014a, b;Xu et al., 2010;Wang et al., 2013;Xu et al., 2012;Liu and Liang, 2005;Serebryany and King, 2014). Dissection of the mechanisms underlying various cataracts will facilitate the development of potential strategies for cataract prevention and treatment. Meanwhile, crystallins have been taken as model proteins in the protein folding field for a long time. Numerous cataract-causing crystallin mutations also provide invaluable information to study the structural determinants of protein folding and aggregation. Previous studies have shown that the integrity of the Greek-key motifs is important to the understanding of β/γ-crystallin folding and misfolding (Vendra et al., 2013;Moreau and King, 2009;Flaugh et al., 2005). This proposal was also reinforced by our results. The mutation at S228, which is the first residue at the last β-strand of Greek-key motif 4, resulted in severe changes in βB1 folding and stability. Our results showed that the mutated protein could not gain the native structure of βB1. The misfolded S228P protein was prone to aggregate and degrade in both human and E. coli cells. Our results strongly suggested that S228 was an essential structural residue for βB1 folding. Unlike the other mutations occurred at residues in the conserved Greek-key motifs, the S228P mutation did not disrupt the hydrophobic core directly. According to the structural studies by MD simulations, we propose that the hydrophobic core of βB1 CTD is protected by surface loops and helices, particularly gated by two interacting loops formed by residues around S173 and G200. Alignment of NTD and CTD structures indicated that NTD also possessed such two gating loops. The distance between the backbones of these two loops was the same for NTD and CTD though the composing residues are not conserved in their primary sequences. The movements of these two loops in WT βB1 were highly restricted but had higher fluctuations in S228P. Structurally, these two loops acted as the "doors" to protect the hydrophobic core. The closure of the doors prevented solvent molecules to access the hydrophobic core and thereby stabilized the dehydrated core structure (Fig. 6F). S228 locked the doors by stabilizing the loop around G200 via forming H-bonding network with Y196, Y198 and Y201. Considering that this Ser can be substituted by Ala in some species (Fig. S1A), it seems that the polar side chain of Ser does not play a major role in S228 functions. The backbone of S228 forms two hydrogen bonds with Y196, which may be important to stabilize these two loops. Alignment of domain structures from various β/γ-crystallins indicated that the positions and interaction of these two loops are highly conserved in the β/γ-crystallin superfamily (Fig. 7). Therefore the shielding effect of these two interacting loops is probably a general structural factor governing the structural stability and folding of all β/γ-crystallin domains. Further research in the other β/γ-crystallins is needed to verity this proposal. It will be interesting to investigate whether the other globular domains also possess similar structures to shield the hydrophobic core from solvent access. Globular proteins are characterized by the burial of apolar residues in the hydrophobic core and the solvation of surface polar residues (Baldwin, 2007;Prabhu and Sharp, 2005). Thus desolvation of residues forming the hydrophobic core is crucial to the folding of globular proteins. Based on our observations, we propose that the final step of domain folding in β/γ-crystallins is to close the doors formed by the two loops and thereby to accomplish the formation of a relatively dry core structure. Failure to close the doors, such as the disruption by the S228P mutation, will retard the completeness of domain folding. This proposal is supported by the significant deviation in the I 2 to I 1 transition of S228P from that of the WT βB1 (Fig. 3). Compared with I 2 of βB1, I 2 of S228P had an about 8 nm red-shift of the Trp fluorescence, indicating that the Trp fluorophores in I 2 of S228P were more solvent accessible. Although the refolded S228P The hydrophobic core of the structurally conserve NTD and CTD is covered by two interacting loops. The S228P mutation separated the two loops in CTD but not those in NTD. The Cα atoms of residues used for distance determination are highlighted by spheres. (E) Distance between the two loops determined from the Cα atoms between N82 and N108 in NTD and their equivalent residues S173 and G200 in CTD. The left panel shows the time-course changes in the distances and the right panel shows the average value of the distances. (F) Open of the interacting loops in S228P allowed water molecules to penetrate in the hydrophobic core of CTD. The water molecules are shown in stick model. (G) S228 formed H-bonding network with three Try residues and stabilize the interacting loops, which is disrupted by the S228P mutation.
had a similar E max value to WT βB1, the dramatically high Trp fluorescence intensity and ANS fluorescence intensity indicated that refolded S228P failed to bury all hydrophobic residues and the fluorescence quenching residues were wrongly positioned. Consequently, the refolded S228P was instable and prone to aggregate at the physiological temperature. It is worth noting that one of the proteolytic sites was around S173, which suggested that the gateless CTD in S228P was more prone to be attacked by water and trace amount of heavy metals or proteases. The susceptibility of S228P C-terminus to degradation also confirmed the proposal that the C-terminal domain was less folded in the S228P mutant.
In summary, we propose that the S228P mutation disrupted the interactions between the two gated doors of the hydrophobic core in CTD. Failure to shield the hydrophobic core from solvent led to a less packed C-terminal domain with extra hydrophobic exposure. The misfolded S228P mutant was prone to degrade and aggregate in the cells, which finally formed large light-scattering particles in the cataractous lens in patients. A possible method to prevent or cure the cataractous lens caused by the S228P mutation is to develop lanosterol-based drugs since lanosterol could redissolve intracellular S228P aggregates, no matter whether they were p62-positive or p62-negative.

Protein sample preparation
Human CRYBB1 and CRYBA3 genes were cloned from the human lens cDNA library as described previously . The S228P mutant of βB1 was obtained by site-directed mutagenesis using the following primers: For-βB1, 5′-CGGGATCCATGTCTCAG GCTGCAAAGGC-3′ and Rev-βB1, 5′-CCCAAGCTTTCACTTGGGG GGCTCTGTG-3′. The C-terminal truncated mutants of βB1 were constructed using the following primers: For-βB1 1-142 , 5′- The sphere models show that the interaction between the two loops is conserved across various β/γ-crystallins. The structurally equivalent loops could also be found in NTD of various β/γ-crystallins (Data not shown).

Refolding of proteins from the inclusion bodies
Since the intact S228P proteins mainly existed in inclusion bodies when overexpressed in E. coli cells, the non-proteolytic S228P proteins were obtained by refolding from the inclusion bodies by dilution. The inclusion bodies were separated from the precipitation fractions of cell lysates in the presence of 1 mmol/L PMSF and washed by buffer B (20 mmol/L sodium phosphate, 500 mmol/L NaCl, 1 mol/L GdnHCl and 0.5% Triton-100, pH 7.0) for three times to remove the unexpected proteins and membranes. The purified inclusion bodies were dissolved in buffer C (20 mmol/L sodium phosphate, 500 mmol/L NaCl and 6 mol/L GdnHCl, pH 7.0). The Histagged proteins in the inclusion bodies were purified by Ni 2+ -affinity chromatography and gel filtration chromatography using the same procedures as those for the purification of soluble proteins except that all buffers contained 6 mol/L GdnHCl. The purified 6 mol/L GdnHCl-denatured proteins were concentrated by PEG20000 to a final concentration of 20 mg/mL. Protein refolding was performed by diluting the 6 mol/L GdnHCl-denatured proteins in buffer A with a dilution ratio of 1:100. The refolding solutions were incubated at 4°C for 20 h and then the supernatant fractions were collected. The refolded proteins were further purified by gel filtration chromatography at 4°C and the peaks with >95% purity were collected, concentrated and stored at −80°C.

Spectroscopic experiments
Far-UV circular dichroism (CD) was recorded on a Chirascan spectrophotometer using a 0.1 cm path-length cell. The samples for the far-UV CD experiments were in buffer D (2 mmol/L sodium phosphate, 15 mmol/L NaCl, 0.1 mmol/L EDTA and 0.1 mmol/L DTT, pH 7.0) to reduce the influence of high concentration of salts. The fluorescence spectra measured were on a Hitachi F-2500 spectrofluorimeter using a 1 cm path-length curvette. The intrinsic Trp fluorescence was excited by 295 nm light, while the extrinsic ANS fluorescence was excited by 380 nm light. The samples for ANS fluorescence measurements were prepared by adding 1:75 ANS probes to the protein solutions. Parameter A, which is a sensitive monitor of the changes in the shape and position of Trp fluorescence spectra (Turoverov et al., 1976), was calculated by dividing the fluorescence intensity at 320 nm by that at 365 nm (I 320 /I 365 ). Resonance Raleigh light scattering was measured using an excitation wavelength of 295 nm as described previously (He et al., 2009). Turbidity was determined by the absorbance at 400 nm of the protein solutions on an Ultraspec 4300 pro UV/Visible spectrophotometer. Most spectroscopic experiments were performed using a protein concentration of 5.8 µmol/L (∼0.2 mg/mL) unless otherwise indicated.

SEC and SDS-PAGE analysis
The SEC analysis was performed using a Superdex 200 column equipped on an ÄKTA FPLC with an elution rate of 0.5 mL/min. About 100 µL protein solutions were injected in the column. The protein concentration for SEC analysis was 30 µmol/L (∼1 mg/mL) and buffer A was used as the elution buffer. The SDS-PAGE analysis was performed using the 12.5% separating gel. About 40 µL protein solutions with a protein concentration of 0.2 mg/mL were used for the SDS-PAGE analysis.

Protein refolding experiments
The fully denatured proteins were prepared by incubating the proteins in buffer A containing 6 mol/L GdnHCl at 25°C for 24 h. The equilibrium refolding of the proteins were performed by diluting the fully denatured proteins in buffers with various final concentrations of GdnHCl ranging from 0.1 mol/L to 6 mol/L at 4°C overnight. The transition curves of protein equilibrium refolding were obtained by monitoring the GdnHCl-dependence of changes in far-UV CD, intrinsic Trp fluorescence, extrinsic ANS fluorescence, light scattering and turbidity. The appearance of aggregates during kinetic refolding was measured by time-course change in turbidity after protein refolding initiated by 1:50 manual dilution of the 6 mol/L GdnHCl-denatured proteins into buffer A. The turbidity data were recorded every 2 s for 10 min. After 1 h refolding, the samples were centrifuged and analyzed by 12.5% SDS-PAGE. The final GdnHCl and protein concentration were 0.12 mol/L and 0.2 mg/mL, respectively.

Protein aggregation kinetics at the physiological temperature
Aggregation kinetics at the physiological temperature was determined by incubating 0.2 mg/mL homomers or heteromers at 37°C for 50 min. The temperature was controlled by a water bath. The timecourse change in turbidity was recorded on an Ultraspec 4300 pro UV/Visible spectrophotometer every 2 s during the incubation of protein solutions at 37°C. After 50 min incubation, the reaction was terminated on ice and was further analyzed by SEC and SDS-PAGE.

Cell culture and immunofluorescence
The genes of the CRYBB1 and S228P were constructed into the eukaryotic vector pEGFP-C3, pEGFP-N1 or pcDNA 3.1 N-Flag. The HeLa and HEK 293T cell lines were from ATCC. The recombinant plasmids were exogenously expressed in HEK 293T cells by transient transfection using lipofectamine 3000. The intracellular distribution of the exogenously expressed proteins was studied using the same procedure as those reported previously Xi et al., 2015). In brief, the 293T cells were cultured in the DMEM medium with the addition of 10% FBS at 37°C in 5% CO 2 incubator. After transient transfection by lipofectamine 3000 for 6 h, the cells were transferred to fresh DMEM medium with the addition of 10% FBS and cultivated at 37°C for 24 h. Then the transfected cells were washed by PBS buffer for three times, fixed by 4% paraformaldehyde for 40 min, washed three times by PBS buffer, treated by 0.4% Trition X-100 for 10 min and blocked by 10% FBS for 40 min. The fixed cells were stained by dyes and antibodies, followed by washing three times using the PBS buffer. The nuclei were dyed with Hoechst 33342. The aggresomes were detected by the marker protein p62, which was recognized by Alexa 649-conjugated goat antibody against mouse p62. The exogenously expressed proteins were visualized by the tagged GFP. The distribution of the proteins in the cells was studied by a Carl Zeiss LSM 710 confocal microscope. The percentage of cells with aggregates was qualified by calculating the percentages from 200 positively transfected cells in ten randomly selected viewing fields. The mean fluorescence intensity of transfected cells was calculated by ImageJ (Collins, 2007).

Cell apoptosis assay
The cell viability was determined by Cell Counting Kit-8 (CCK-8) using the same procedure as those reported previously . In brief, the HeLa cells were transiently transfected by the empty vector pcDNA 3.1 N-Flag and the recombinant plasmids containing the WT CRYBB1 and S228P genes. The transfected cells were cultured at 37°C for 24 h and then were double stained by propidium iodide (PI) and annexin V. The percentages of apoptotic and necrotic cells were analyzed by bivariate flow cytometry on a FACSCalibur flow cytometer from BD Biosciences.

Molecular dynamic simulations
Details of molecular dynamic (MD) simulation analysis were the same as those described previously (Wang et al., 2013;Chen et al., 2012). In brief, the starting structure of the mutant S228P was constructed by single site mutagenesis by PyMOL (The PyMOL Molecular Graphics System, Version 0.99rc6, Schrödinger, LLC, http://www.pymol.org/) using the crystal structure of truncated human βB1 (PDB ID: 1OKI) (Montfort et al., 2003) as the template. MD simulations were performed in a water box containing 150 mmol/L NaCl generated by Visual molecular dynamics (VMD) encoded in Nanosecond molecular dynamics (NAMD), version 2.9 (Phillips et al., 2005). After equilibration of the system for 5.8 ns, the simulations were run at 2 fs time-steps for 20 ns at 310 K and constant pressure (1 atm). The simulated structures were analyzed by VMD and PyMOL. Both dimeric structure and subunit structure (monomer) were applied to MD simulations to evaluate the effects of the S228P mutation on structures of the Greek-key motifs and dimer assembly.