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Polyethylenimine-poly(amidoamine) dendrimer modified with l-arginines as an efficient gene delivery vector

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Abstract

In this study, we synthesized polyethylenimine-polyamidoamine-arginine dendritic polymers (PPRs) as vectors for gene delivery. Four polymers, polyethylenimine-polyamidoamine generation 1 (PP1), PP2, PP1-arginine (PP1R), and PP2-arginine (PP2R), were synthesized and confirmed by 1H NMR. PPRs were shown to interact with and condense plasmid DNA effectively to form 171-179 nm polyplexes with 30-32 mV of zeta potentials at weight ratio 4:1 (polymer:plasmid DNA). Cytotoxicity of PPRs/pDNA complexes was lower than that of polyethylenimine (PEI) 25 kDa/pDNA complexes for all concentration ranges tested. In 293 cells, PP1R/pDNA complexes showed higher gene transfection efficiency than PEI 25 kDa. These results suggest that PPR could be promising dendritic gene carriers for gene therapy.

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Authors and Affiliations

  1. Department of Biochemistry, Chungnam National University, Daejeon, 305-764, Korea

    Nan Young Ahn, Tae-Hun Kim, Su Jeong Song, Jeong-Mi Moon & Joon Sig Choi

  2. Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Korea

    Joon Sig Choi

  3. Future Biotechnology Research Division, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Daejeon, 305-806, Korea

    Tai Hwan Ha

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  1. Nan Young Ahn
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  2. Tae-Hun Kim
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  3. Su Jeong Song
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  4. Jeong-Mi Moon
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  5. Tai Hwan Ha
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  6. Joon Sig Choi
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Correspondence to Tai Hwan Ha or Joon Sig Choi.

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These authors contributed equally to this work.

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Ahn, N.Y., Kim, TH., Song, S.J. et al. Polyethylenimine-poly(amidoamine) dendrimer modified with l-arginines as an efficient gene delivery vector. Macromol. Res. 23, 726–733 (2015). https://doi.org/10.1007/s13233-015-3101-6

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  • DOI: https://doi.org/10.1007/s13233-015-3101-6

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