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Optimization of chitinase production by a new Streptomyces griseorubens C9 isolate using response surface methodology

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Abstract

The purpose of this article is to use statistical Plackett–Burman and Box–Wilson response surface methodology to optimize the medium components and, thus, improve chitinase production by Streptomyces griseorubens C9. This strain was previously isolated and identified from a semi-arid soil of Laghouat region (Algeria). First, syrup of date, colloidal chitin, yeast extract and K2HPO4, KH2PO4 were proved to have significant effects on chitinase activity using the Plackett–Burman design. Then, an optimal medium was obtained by a Box–Wilson factorial design of response surface methodology in liquid culture. Maximum chitinase production was predicted in medium containing 2% colloidal chitin, 0.47% syrup of date, 0.25 g/l yeast extract and 1.81 g/l K2HPO4, KH2PO4 using response surface plots of the STATISTICA software v.12.0.

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Acknowledgements

We thank Prof. A. Boulahrouf, head of applied microbiology team at the engineering microbiology and applications laboratory (Constantine University) for helping us in this work. Thanks are expressed to France Chitin (www.france-chitine.com) for a gift of chitin. We also thank M. Merouane Fateh and M. Mezdour Hichem (Constantine University) for their technical advice. This work was supported by Mentouri Brothers University Constantine, Algeria.

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  1. Gasmi Meriem & Kitouni Mahmoud

    Present address: Department of Biochemistry and Cellular Biology, University of Mentouri Brothers, Constantine, Algeria

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  1. Laboratoire de Génie microbiologie et applications, Université des Frères Mentouri Constantine, Campus Chaâbat Erssas, Route Ain El Bey, 25000, Constantine, Algeria

    Gasmi Meriem & Kitouni Mahmoud

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  1. Gasmi Meriem
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Correspondence to Gasmi Meriem.

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Meriem, G., Mahmoud, K. Optimization of chitinase production by a new Streptomyces griseorubens C9 isolate using response surface methodology. Ann Microbiol 67, 175–183 (2017). https://doi.org/10.1007/s13213-016-1249-8

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  • DOI: https://doi.org/10.1007/s13213-016-1249-8

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