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Effect of mustard rhizobacteria on wheat growth promotion under cadmium stress: characterization of acdS gene coding ACC deaminase

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Abstract

Ten mustard rhizobacterial isolates that utilize 1-aminocyclopropane-1-carboxylate (ACC) as sole nitrogen source were screened for plant growth-promoting traits. These isolates enhanced root elongation significantly and minimized ethylene synthesis in wheat seedlings under induced cadmium stress condition. The presence of acdS gene coding for ACC deaminase was tested through gene-specific PCR amplification and further confirmed by nucleic acid hybridization. acdS gene from isolates Ps 2-3 and Ps 7-12 selected on the basis of wheat growth promotion was cloned and sequenced. Sequence analysis showed an open reading frame of 1017 bp encoding a complete polypeptide with an identity of 86.4 % between each other and sharing 84–95 % similarity with the earlier reported acdS genes. The deduced protein sequences were highly conserved and shared a 95.2 % homology at the amino acid level. Eight and thirteen new amino acid residues were found in the protein sequence of Ps 2-3 and Ps 7-12 with variation in numbers of different peptidase acting sites and protein motifs. Based on 16S rDNA sequencing, the isolates Ps 2-3 and Ps 7-12 were identified as Pseudomonas sp. and Pseudomonas fluorescens, respectively.

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Acknowledgments

We are grateful to Prof. Bernard R. Glick, Department of Biology, University of Waterloo, Ontario, Canada, for his valuable suggestions during the course of our investigation. The comments and suggestions of Dr. Tilak R. Sharma, National Research Centre on Plant Biotechnology, New Delhi, India, helped to considerably enhance the manuscript. The first author thanks Indian Agricultural Research Institute, New Delhi, India, for financial assistance in the form of Senior Research Fellowship. A financial grant received from the Department of Science and Technology (DST), India is also gratefully acknowledged.

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The authors of this manuscript declare that they do not have any conflict of interest.

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Authors and Affiliations

  1. Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Venkadasamy Govindasamy, Murugesan Senthilkumar & Kannepalli Annapurna

  2. National Institute of Abiotic Stress Management, Indian Council of Agricultural Research, Malegaon, Baramati (Pune), 413115, Maharashtra, India

    Venkadasamy Govindasamy

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  1. Venkadasamy Govindasamy
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  2. Murugesan Senthilkumar
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  3. Kannepalli Annapurna
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Correspondence to Venkadasamy Govindasamy.

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Govindasamy, V., Senthilkumar, M. & Annapurna, K. Effect of mustard rhizobacteria on wheat growth promotion under cadmium stress: characterization of acdS gene coding ACC deaminase. Ann Microbiol 65, 1679–1687 (2015). https://doi.org/10.1007/s13213-014-1007-8

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  • DOI: https://doi.org/10.1007/s13213-014-1007-8

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