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Cloning and characterization of a differentially expressed mitochondrial manganese superoxide dismutase gene from Pleurotus ostreatus

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Abstract

Manganese-containing superoxide dismutase (Mn-SOD) is one of the most important superoxide dismutases found in many eukaryotes and bacteria. In this study, the full-length cDNA of mitochondrial manganese superoxide dismutase from Pleurotus ostreatus (PoMn-SOD) was obtained. It contained 776 nucleotides with an open reading frame (ORF) of 663 bp, encoding 220 amino acid residues. The deduced amino acid sequence showed high identity with the sequences of other basidiomycetous Mn-SODs from Trametes versicolor (71 %) to Laccaria bicolor (77 %). Quantitative real-time PCR (RT-PCR) analysis revealed that PoMn-SOD gene transcripts were abundant in the stage of young fruit bodies and mature fruit bodies. The up-regulation of the PoMn-SOD gene suggested that it was developmental regulated and could play an important role in antagonizing environmental stresses. In addition, another isoform of Mn-SOD was detected by a non-denaturing polyacrylamide gel electrophoresis (PAGE) approach and the highest total Mn-SOD activity (203.9 U/mg) was observed in the stage of mature fruit bodies. These data could provide important reference for functional study of the PoMn-SOD gene and may benefit biotechnological production of Mn-SOD in the future.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31172011 and No. 30771502). We thank Weifeng Chen for her assistance during the homology modeling.

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Authors and Affiliations

  1. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Chaomin Yin, Wenxian Zhao, Jihong Zhu & Aimin Ma

  2. College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Liesheng Zheng & Liguo Chen

  3. Key Laboratory of Agro-Microbial Resources and Utilization, Ministry of Agriculture, Wuhan, 430070, China

    Aimin Ma

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  1. Chaomin Yin
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Correspondence to Aimin Ma.

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Yin, C., Zhao, W., Zhu, J. et al. Cloning and characterization of a differentially expressed mitochondrial manganese superoxide dismutase gene from Pleurotus ostreatus . Ann Microbiol 65, 1597–1606 (2015). https://doi.org/10.1007/s13213-014-0999-4

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  • DOI: https://doi.org/10.1007/s13213-014-0999-4

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