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Effects of photoperiod, salinity and pH on cell growth and lipid content of Pavlova lutheri

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Abstract

The aim of the present work was to study the effects of photoperiod, salinity and pH on growth and lipid content of Pavlova lutheri microalgae for biodiesel production in small-scale and large-scale open-pond tanks. In a 250-mL flask, the cultures grew well under 24 h illumination with maximum specific growth rate, μ max , of 0.12 day−1 and lipid content of 35 % as compared to 0.1 day−1 and 15 % lipid content in the dark. The salinity was optimum for the cell growth at 30–35 ppt, but the lipid content of 34–36 % was higher at 35–40 ppt. Algal growth and lipid accumulation was optimum at pH 8–9. Large-scale cultivation in 5-L and 30-L tanks achieved μ max of 0.13–0.14 day−1 as compared to 0.12 day−1 in small-scale and 300L cultures.

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Acknowledgments

The authors are grateful to Universiti Teknologi PETRONAS for providing facilities and for the scholarship to Syed Muhammad Usman Shah. Funding through the Fundamental Research Grant Scheme (FRGS/2/2010/TK/UTP/02/14) is acknowledged.

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Authors and Affiliations

  1. Department of Chemical Engineering, Universiti Teknologi PETRONAS, 31750, Bandar Seri Iskandar, Perak, Malaysia

    Syed Muhammad Usman Shah & Mohd Azmuddin Abdullah

  2. Centre of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

    Che Che Radziah

  3. Fisheries Research Institute, 11960, Batu Maung, Pulau Pinang, Malaysia

    Saadiah Ibrahim & Faazaz Latiff

  4. Fisheries Research Institute, Pulau Sayak, 08500, Kota Kuala Muda, Kedah, Malaysia

    Mohd Fariduddin Othman

Authors
  1. Syed Muhammad Usman Shah
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  2. Che Che Radziah
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  3. Saadiah Ibrahim
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  4. Faazaz Latiff
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  5. Mohd Fariduddin Othman
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  6. Mohd Azmuddin Abdullah
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Correspondence to Mohd Azmuddin Abdullah.

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Shah, S.M.U., Che Radziah, C., Ibrahim, S. et al. Effects of photoperiod, salinity and pH on cell growth and lipid content of Pavlova lutheri . Ann Microbiol 64, 157–164 (2014). https://doi.org/10.1007/s13213-013-0645-6

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  • DOI: https://doi.org/10.1007/s13213-013-0645-6

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