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Gentiana scabra extracts stimulate glucagon-like peptide-1 secretion via G protein-coupled receptor pathway

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Abstract

Glucagon-like peptide-1 (GLP-1) which secreted by enteroendocrine L cell have been issued as a therapeutic agent for type II diabetes mellitus, because of its function to stimulate insulin secretion in pancreatic β-cells. It is widely studied that sweet, bitter, and umami taste stimuli induce the secretion of GLP-1 in enteroendocrine L cell via G protein coupled receptor (GPCR) signaling pathway. Gentiana scabra (GS) is one of the bitter tasting herbal medicines which have been used in traditional oriental medicine to treat the diabetes mellitus. This study shows the GLP-1 secretion of the enteroendocrine L cell stimulated by stimulation of GS extractions through GPCR pathway. To examine the molecular mode-of-action, GLP-1 ELISA and microarray was performed. This study provides the understanding the possibility of GS used as a therapeutic herbal medicine for the type II diabetes.

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References

  1. Jang, H.J. et al. Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proc. Natl. Acad. Sci. USA 104, 15069–15074 (2007).

    Article  CAS  Google Scholar 

  2. Martin, B. et al. Modulation of taste sensitivity by GLP-1 signaling in taste buds. Ann. N. Y. Acad. Sci. 1170, 98–101 (2009).

    Article  CAS  Google Scholar 

  3. Toft-Nielsen, M.B., Madsbad, S. & Holst, J.J. Determinants of the effectiveness of glucagon-like peptide-1 in type 2 diabetes. J. Clin. Endocrinol. Metab. 86, 3853–3860 (2001).

    Article  CAS  Google Scholar 

  4. Leech, C.A. et al. Molecular physiology of glucagonlike peptide-1 insulin secretagogue action in pancreatic beta cells. Prog. Biophys. Mol. Biol. 107, 236–247 (2011).

    Article  CAS  Google Scholar 

  5. Kinnamon, S.C. Taste receptor signalling — from tongues to lungs. Acta Physiol (Oxf) 204, 158–168 (2011).

    Article  Google Scholar 

  6. Hurowitz, E.H. et al. Genomic characterization of the human heterotrimeric G protein alpha, beta, and gamma subunit genes. DNA Res. 7, 111–120 (2000).

    Article  CAS  Google Scholar 

  7. Zhang, J.-q. On the study on treatment of diabetes with traditional Chinese medicine. Chinese Journal of Integrative Medicine 2, 82–83 (1995).

    Google Scholar 

  8. Goldsmith, Z.G. & Dhanasekaran, D.N. G protein regulation of MAPK networks. Oncogene 26, 3122–3142 (2007).

    Article  CAS  Google Scholar 

  9. Daulhac, L., Kowalski-Chauvel, A., Pradayrol, L., Vaysse, N. & Seva, C. Src-family tyrosine kinases in activation of ERK-1 and p85/p110-phosphatidylinositol 3-kinase by G/CCKB receptors. J. Biol. Chem. 274, 20657–20663 (1999).

    Article  CAS  Google Scholar 

  10. Xu, Y. & Casey, G. Identification of human OGR1, a novel G protein-coupled receptor that maps to chromosome 14. Genomics 35, 397–402 (1996).

    Article  CAS  Google Scholar 

  11. Martin, S.C., Yule, D.I., Dunne, M.J., Gallacher, D.V. & Petersen, O.H. Vasopressin directly closes ATP-sensitive potassium channels evoking membrane depolarization and an increase in the free intracellular Ca2+ concentration in insulin-secreting cells. EMBO J. 8, 3595–3599 (1989).

    CAS  Google Scholar 

  12. Lo Vasco, V. 1p36.32 rearrangements and the role of PI-PLC η2 in nervous tumours. Journal of Neuro-Oncology 103, 409–416 (2011).

    Article  Google Scholar 

  13. Andrikopoulos, P. et al. Ca2+ influx through reverse mode Na+/Ca2+ exchange is critical for vascular endothelial growth factor-mediated extracellular signal-regulated kinase (ERK) 1/2 activation and angiogenic functions of human endothelial cells. J. Biol. Chem. 286, 37919–37931.

  14. Kim, K.S. et al. The multi-target antibiotic efficacy of Angelica dahurica Bentham et Hooker extract exposed to the Escherichia coli O157:H7 BioChip J. 5, 333–342 (2011).

    Article  CAS  Google Scholar 

  15. Choi, E.K. et al. Genome-wide gene expression analysis of Patrinia scabiosaefolia reveals an antibiotic effect. BioChip J. 5, 246–254 (2011).

    Article  CAS  Google Scholar 

  16. Yang, H.J. et al. Global transcriptome analysis of the E. coli O157 response to Agrimonia pilosa extract. Molecular & Cellular Toxicology 7, 299–310 (2011).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee University, Heogi-dong, Dongdaemun-gu, Seoul, 130-701, Korea

    Min-Hee Shin, Hyo-Weon Suh, Ki-Beom Lee, Ki-Suk Kim, Hea Jung Yang, Eun-Kyeong Choi, Yu Jeong Cho, Mi-Yeon Song, Kwang Seok Ahn & Hyeung-Jin Jang

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  1. Min-Hee Shin
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  2. Hyo-Weon Suh
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  3. Ki-Beom Lee
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  4. Ki-Suk Kim
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  5. Hea Jung Yang
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  6. Eun-Kyeong Choi
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  7. Yu Jeong Cho
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  8. Mi-Yeon Song
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  9. Kwang Seok Ahn
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  10. Hyeung-Jin Jang
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Correspondence to Hyeung-Jin Jang.

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These authors contributed equally to this work.

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Shin, MH., Suh, HW., Lee, KB. et al. Gentiana scabra extracts stimulate glucagon-like peptide-1 secretion via G protein-coupled receptor pathway. BioChip J 6, 114–119 (2012). https://doi.org/10.1007/s13206-012-6202-8

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  • DOI: https://doi.org/10.1007/s13206-012-6202-8

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