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Pectin-like polysaccharide extracted from leaves Crataeva tapia promotes antioxidant, immunomodulatory and emulsifiers applied in therapeutic formulations

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Abstract

The objective of this work was to isolate a polysaccharide similar to pectin from Crataeva tapia leaves, not yet reported in the literature, and to evaluate its antioxidant, cytotoxic and immunomodulatory profile. Pectin was extracted from the leaves in three stages, organic solvent followed by acidified water and ethanol precipitation. With the pectin obtained, the physicochemical characterization of the molecule was carried out using high-performance liquid chromatography, Fourier-transform infrared spectroscopy, nuclear magnetic resonance (13C and 1H) and different thermal and elemental analysis. Furthermore, the antioxidant activities were evaluated in vitro, and using human peripheral blood mononuclear cell culture, cytotoxicity and immunostimulatory actions were investigated. Physical and chemical analyses showed characteristic signs of pectin. Antioxidant activity tests showed that pectin had moderate to low antioxidant activity. Furthermore, pectin did not affect the viability of erythrocytes and PBMC and induced an immunostimulatory state when it promoted the production of cytokines IL-6, IL-10 and TNF-α and increased the activation of CD8 + T lymphocytes. This study showed that pectin from Crataeva tapia is not cytotoxic and promoted a pro-inflammatory profile in peripheral blood mononuclear cell with application as an immunostimulating and emulsifying compound.

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Acknowledgements

We would like to thank CAPES (Coordination for the Improvement of Higher Education Personnel) and the Pernambuco State Research Support Foundation—FACEPE (Process APQ-0498-4.03/19), researcher fixation grant—FACEPE (Process BFP-0038-04.03/21) and grant from the National Council for Science and Technology Development—CNPq (Process 306865/2020-3) for granting financial assistance during the research period. The authors also thank the Center for Technological Platforms at the Aggeu Magalhães Research Center for using the flow cytometer (FIOCRUZ Pernambuco).

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  1. Department of Antibiotics, Biosciences Center, Federal University of Pernambuco, Recife, PE, 50.670-420, Brazil

    Simone da Paz Leôncio Alves, Iris Trindade Tenório Jacob, Marcela Daniela Muniz Arruda, Georon Ferreira de Sousa, Guilherme Antônio de Souza, Maria do Carmo Alves de Lima, Ivone Antônia de Souza, Cristiane Moutinho Lagos de Melo, Iranildo José da Cruz Filho & Dayane Kelly Dias do Nascimento Santos

  2. Department of Biochemistry, Biosciences Center, Federal University of Pernambuco, Recife, PE, 50.670-420, Brazil

    Abdênego Rodrigues da Silva

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  1. Simone da Paz Leôncio Alves
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Correspondence to Dayane Kelly Dias do Nascimento Santos.

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da Paz Leôncio Alves, S., Jacob, I.T.T., Arruda, M.D.M. et al. Pectin-like polysaccharide extracted from leaves Crataeva tapia promotes antioxidant, immunomodulatory and emulsifiers applied in therapeutic formulations. 3 Biotech 13, 114 (2023). https://doi.org/10.1007/s13205-023-03509-y

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