Abstract
The bacterium Microbulbifer sp. ALW1 was previously characterized with the capability to break down the cell wall of brown algae into fine pieces. The biological functions of strain ALW1 were yet to be elucidated. In this study, a gene, namely MaCel5A, was isolated from the ALW1 strain genome, encoding an endo-β-1,4-glucanase. MaCel5A was phylogenetically categorized under the glycoside hydrolase family GH5, with the highest identity to a putative cellulase of Microbulbifer thermotolerans. The recombinant MaCel5A protein purified from heterologous expression in E. coli exhibited maximum activity at 50 °C and pH 6.0, respectively, and functioned selectively toward carboxymethyl cellulose and barley β-glucan. Recombinant MaCel5A demonstrated considerable tolerance to the exposure to high temperature up to 80 °C for 30 min retaining 49% residual activity. In addition, MaCel5A showed moderate stability against pH 5.0–11.0 and strong stability in the presence of nonionic surfactant. MaCel5A exhibited strong halo-stability and halotolerance. The activity of the enzyme increased about tenfold at 0.5 M NaCl, and about fivefold even at 4.0 M NaCl compared to the enzyme activity without the addition of salt. The two conserved glutamic acid residues in MaCel5A featured the typical catalytic acid/base and nucleophile machinery of glycoside hydrolases. These characteristics highlight the industrial application potential of MaCel5A.
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Acknowledgements
This work was financially supported by the Natural Science Foundation of Fujian Province of China (no. 2020J01679), and the Open Research Foundation of Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, China (no. B18097-6).
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HL: conceptualization, methodology, validation, writing—original draft, funding acquisition. QH and XH: investigation, formal analysis. ZJ: Writing—review and editing. HN: conceptualization, writing—review and editing, supervision, project administration. QL: supervision, writing—review and editing. YZ: conceptualization, writing—review and editing, supervision, funding acquisition.
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Li, H., Hu, Q., Hong, X. et al. Molecular cloning and characterization of a thermostable and halotolerant endo-β-1,4-glucanase from Microbulbifer sp. ALW1. 3 Biotech 11, 250 (2021). https://doi.org/10.1007/s13205-021-02801-z
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DOI: https://doi.org/10.1007/s13205-021-02801-z