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RNAi activation with homologous and heterologous sequences that induce resistance against the begomovirus Pepper golden mosaic virus (PepGMV)

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Abstract

This study compared the transcriptional changes in Nicotiana benthamiana plants treated with homologous sequences derived from Pepper golden mosaic virus (PepGMV) and heterologous sequences that derived from another begomovirus, Tomato chino La Paz virus (ToChLPV) prior to infection by PepGMV. The results of microarray analyses identified upregulated genes associated with RNAi such as DCL2, DCL4, AGO3, AGO7, AGO10, NRPD2B (Pol IV), DRB3, CMT3, RDR6. The components that participate in different RNAi pathways were identified, including methylation induced by both constructs, as well as the code of these genes in Arabidopsis thaliana and its counterpart in N. benthamiana through different genome assembly. The expression of these genes was validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR), where DCL3, DCL4, AGO1-1, AGO2, RDR6 and PPR1 showed increased expression during plant protection with the heterologous construct compared to those protected with the homologous construct. The results of this study confirmed the activation of the gene silencing mechanism at the transcriptional level with both constructs and established the possibility of their use as a protection system for both homologous and heterologous sequences.

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Acknowledgements

This research was financed by the Consejo Nacional de Ciencia y Tecnología (CONACyT, Mexico) through projects SEP2007-84777 and SEP2015-253828 “Analysis of the regulatory function of sRNAs in a gene silencing system as a mechanism of protection against mixed begomoviral infections” granted to R.J. Holguín-Peña. The authors are grateful to Gerardo Rafael Hernández García for his support in figure editing, the Laboratory of Phytopathology, CIBNOR, México, and Diana Fischer for editorial services.

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Authors and Affiliations

  1. Centro de Investigaciones Biológicas del Noroeste, Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, 23096, La Paz, Baja California Sur, Mexico

    Mayela Vargas-Salinas, Diana Medina-Hernández, Guadalupe Fabiola Arcos-Ortega & Ramón Jaime Holguín-Peña

  2. Facultad de Ciencias del Mar, Universidad Autónoma de Sinaloa (UAS) Mazatlán, 82000, Sinaloa, Mexico

    Irasema Elizabeth Luis-Villaseñor

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  1. Mayela Vargas-Salinas
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  2. Diana Medina-Hernández
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  3. Guadalupe Fabiola Arcos-Ortega
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  4. Irasema Elizabeth Luis-Villaseñor
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  5. Ramón Jaime Holguín-Peña
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by Diana Medina Hernández, Mayela Vargas Salinas, Guadalupe Fabiola Arcos Ortega and Irasema Elizabeth Luis Villaseñor. The first draft of the manuscript was written by Diana Medina Hernández, Mayela Vargas Salinas, Guadalupe Fabiola Arcos Ortega and all authors commented on previous versions of the manuscript. Review and editing: Mayela Vargas Salinas and Guadalupe Fabiola Arcos Ortega. Funding acquisition: Ramón Jaime Holguín Peña. All authors read and approved the final manuscript.

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Correspondence to Ramón Jaime Holguín-Peña.

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Vargas-Salinas, M., Medina-Hernández, D., Arcos-Ortega, G.F. et al. RNAi activation with homologous and heterologous sequences that induce resistance against the begomovirus Pepper golden mosaic virus (PepGMV). 3 Biotech 11, 114 (2021). https://doi.org/10.1007/s13205-021-02653-7

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