Abstract
In the present study, we fabricated epigallocatechin-3-gallate (EGCG) loaded albumin nanoparticles (Alb-NP-EGCG) to enhance bioavailability and improve pharmacokinetic parameters of EGCG. The physicochemical properties of the Alb-NP-EGCG were studied using scanning electron microscopy, differential scanning calorimetry, powder X-ray diffraction and in vitro release studies. Characterization of Alb-NP-EGCG indicated the formation of spherical nanoparticles with no drug and excipient interaction. Alb-NP-EGCG showed a high drug loading capacity of 92%. Further, in vitro study showed a sustained release of EGCG from Alb-NP-EGCG over a period of 48 h. Mathematical modeling and release kinetics indicated that the Alb-NP-EGCG followed zero order kinetic and EGCG was released via fickian diffusion method. In vivo bioavailability and distribution of Alb-NP-EGCG showed an enhanced plasma concentration of EGCG with 1.5 fold increase along with prolonged T1/2 of 15.6 h in the system when compared with the free EGCG. All this study demonstrated the fabrication of EGCG loaded albumin nanoparticles which favored the slow and sustained release of EGCG with improved pharmacokinetics and bioavailability thereby prolonging the action of EGCG. Additional acute and sub-acute toxicity test of the Alb-NP-EGCG demonstrated the safety of the Alb-NP-EGCG. Therefore, the Alb-NP-EGCG could be a promising drug delivery system for EGCG.
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Acknowledgements
The authors are thankful to NTRF, Tea Board, Kolkata for providing financial support. The authors are also thankful to the management of VIT for providing necessary facilities. We would also thank Sun Pharmaceutical Industrial limited, India for sample analysis.
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Funding was provided by National Tea Research Foundation, Tea Board, Kolkata (17(330)/214).
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AKAM was responsible for the concept and design of this study. NR performed all the experiments and compiled the results. NR and AKAM analyzed the data and drafted the manuscript. AKAM performed the final critical revision of the manuscript. All authors have read and approved the final manuscript.
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Ramesh, N., Mandal, A.K.A. Encapsulation of epigallocatechin-3-gallate into albumin nanoparticles improves pharmacokinetic and bioavailability in rat model. 3 Biotech 9, 238 (2019). https://doi.org/10.1007/s13205-019-1772-y
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DOI: https://doi.org/10.1007/s13205-019-1772-y