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Growth, substrate consumption, and product formation kinetics of Phanerochaete chrysosporium and Schizophyllum commune mixed culture under solid-state fermentation of fruit peels

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Abstract

Kinetic analysis of solid-state fermentation (SSF) of fruit peels with Phanerochaete chrysosporium and Schizophyllum commune mixed culture was studied in flask and 7 kg capacity reactor. Modified Monod kinetic model suggested by Haldane sufficiently described microbial growth with co-efficient of determination (R2) reaching 0.908 at increased substrate concentration than the classical Monod model (R2 = 0.932). Leudeking–Piret model adequately described product synthesis in non-growth-dependent manner (R2 = 0.989), while substrate consumption by P. chrysosporium and S. commune fungal mixed culture was growth-dependent (R2 = 0.938). Hanes–Woolf model sufficiently represented α-amylase and cellulase enzymes synthesis (R2 = 0.911 and 0.988); α-amylase had enzyme maximum velocity (Vmax) of 25.19 IU/gds/day and rate constant (Km) of 11.55 IU/gds/day, while cellulase enzyme had Vmax of 3.05 IU/gds/day and Km of 57.47 IU/gds/day. Product yield in the reactor increased to 32.65 mg/g/day compared with 28.15 mg/g/day in shake flask. 2.5 cm media thickness was adequate for product formation within a 6 day SSF in the tray reactor.

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Acknowledgements

The investigators would like to thank Research Management Center of International Islamic University Malaysia for their financial support in making this work a reality.

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Authors and Affiliations

  1. Department of Biotechnology Engineering, Faculty of Engineering, Bioenvironmental Research Centre (BERC), International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia

    Kola Saheed Olorunnisola, Parveen Jamal & Md. Zahangir Alam

  2. Biological Sciences Department, Elizade University, P.M.B. 002, Ilara-Mokin, Ondo State, Nigeria

    Kola Saheed Olorunnisola

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  1. Kola Saheed Olorunnisola
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Correspondence to Kola Saheed Olorunnisola.

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Olorunnisola, K.S., Jamal, P. & Alam, M. Growth, substrate consumption, and product formation kinetics of Phanerochaete chrysosporium and Schizophyllum commune mixed culture under solid-state fermentation of fruit peels. 3 Biotech 8, 429 (2018). https://doi.org/10.1007/s13205-018-1452-3

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