Abstract
The science of DNA nanotechnology has led to the synthesis of size-controllable materials with uniformed geometries at nano- and micro-scales. Herein, we proposed the double-crossover (DX), antiparallel, and even half-turns perimeter tiles (DAE tiles) to synthesize intact, mono-crystalline, and giant 2D DNA micro-assemblies. The DNA tiles with 10-half-turns perimeter were synthesized via self-assembly of 106 nucleotides (NT) circular scaffold along with the complimentary staple strands. The DAE DNA tiles were successfully polymerized to achieve stable lattices by adjusting the inter-tile distances of certain lengths for attaining torsional (or twisting) chirality. We determined that the inter-tile connections affected the degree of coiling (or super-coiling) and twisting forces (right- or left-handed twists) in the DNA helix. While the degree of polymerization of DNA tiles was also tune-able by controlling the lengths and structural designs of the circular core of the DNA tiles. Furthermore, the number of half-turns in the core and on the connection arms (4 or 5) with even “E” or odd “O” half-turns was crucial. It affected the direction of winding of DNA duplexes to alter the overall stiffness and sturdiness of DNA lattices. The number of half-turns in the connections were either “4 or Even (21 NT); E with 5 NT sticky ends” or “5 Odd (26 NT); O with 6 NT sticky ends” (DAE-E or DAE-O tile systems). The AFM results revealed that the above tile systems (DAE-E or DAE-O) together with the locations of crossovers, and holiday junctions along the DNA tiles controlled the left- or right-handed coiling of DNA double strands. This phenomenon affected the compactness of resulting DNA motifs, the overall intrinsic curvatures, double-strand packing, and the geometry of DNA lattices.
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References
Aghebat Rafat A, Pirzer T, Scheible MB et al (2014) Surface-assisted large-scale ordering of DNA origami tiles. Angew Chemie Int Ed 53:7665–7668. https://doi.org/10.1002/anie.201403965
Ali MM, Li F, Zhang Z et al (2014) Rolling circle amplification: a versatile tool for chemical biology, materials science and medicine. Chem Soc Rev 43:3324–3341
Baig MMFA, Dissanayaka WL, Zhang C (2021a) 2D DNA nanoporous scaffold promotes osteogenic differentiation of pre-osteoblasts. Int J Biol Macromol 188:657–669. https://doi.org/10.1016/j.ijbiomac.2021.07.198
Baig MMFA, Zhang C, Akhtar MF et al (2021b) Treatment of Wilms’ nephroblastoma cancer cells via EGFR targeting of dactinomycin loaded DNA-nanowires. J Pharm Investig 51:233–242. https://doi.org/10.1007/s40005-020-00509-5
Barua R, Das S (2003) Finite field arithmetic using self-assembly of DNA tilings. In: 2003 Congress on Evolutionary Computation, CEC 2003—Proceedings. pp 2529–2536
Zion MY Ben, He X, Maass CC, et al (2017) Self-assembled three-dimensional chiral colloidal architecture
Brady RA, Brooks NJ, Foderà V et al (2018) Amphiphilic-DNA platform for the design of crystalline frameworks with programmable structure and functionality. J Am Chem Soc 140:15384–15392. https://doi.org/10.1021/jacs.8b09143
Dohno C, Makishi S, Nakatani K, Contera S (2017) Amphiphilic DNA tiles for controlled insertion and 2D assembly on fluid lipid membranes: the effect on mechanical properties. Nanoscale 9:3051–3058. https://doi.org/10.1039/c6nr07084a
Hong F, Jiang S, Lan X et al (2018) Layered-crossover tiles with precisely tunable angles for 2D and 3D DNA crystal engineering. J Am Chem Soc 140:14670–14676. https://doi.org/10.1021/jacs.8b07180
Kempter S, Khmelinskaia A, Strauss MT et al (2019) Single particle tracking and super-resolution imaging of membrane-assisted stop-and-go diffusion and lattice assembly of DNA origami. ACS Nano 13:996–1002. https://doi.org/10.1021/acsnano.8b04631
Li M, Zuo H, Yu J et al (2017) One DNA strand homo-polymerizes into defined nanostructures. Nanoscale 9:10601–10605. https://doi.org/10.1039/c7nr03640j
Lopez-Gomollon S, Nicolas FE (2013) Purification of DNA oligos by denaturing polyacrylamide gel electrophoresis (PAGE). Methods Enzymol 529:65–83. https://doi.org/10.1016/B978-0-12-418687-3.00006-9
Lubbe AS, Liu Q, Smith SJ et al (2018) Photoswitching of DNA hybridization using a molecular motor. J Am Chem Soc 140:5069–5076. https://doi.org/10.1021/jacs.7b09476
Mao X, Li K, Liu M et al (2019) Directing curli polymerization with DNA origami nucleators. Nat Commun 10:1–5. https://doi.org/10.1038/s41467-019-09369-6
Marin-Gonzalez A, Vilhena JG, Moreno-Herrero F, Perez R (2019) DNA crookedness regulates DNA mechanical properties at short length scales. Phys Rev Lett 122:1–8. https://doi.org/10.1103/PhysRevLett.122.048102
Marras AE, Zhou L, Su HJ, Castro CE (2015) Programmable motion of DNA origami mechanisms. Proc Natl Acad Sci U S A 112:713–718. https://doi.org/10.1073/pnas.1408869112
Martens K, Binkowski F, Nguyen L et al (2021) Long- and short-ranged chiral interactions in DNA-assembled plasmonic chains. Nat Commun 12:1–9. https://doi.org/10.1038/s41467-021-22289-8
Mitchell JC, Harris JR, Malo J et al (2004) Self-assembly of chiral DNA nanotubes. J Am Chem Soc 126:16342–16343. https://doi.org/10.1021/ja043890h
Nakayama Y, Yamaguchi H, Einaga N, Esumi M (2016) Pitfalls of DNA quantification using dnabinding fluorescent dyes and suggested solutions. PLoS ONE 11:e0150528. https://doi.org/10.1371/journal.pone.0150528
O’Neill P, Rothemund PWK, Kumar A, Fygenson DK (2006) Sturdier DNA nanotubes via ligation. Nano Lett 6:1379–1383. https://doi.org/10.1021/nl0603505
Ohya Y, Miyoshi N, Hashizume M et al (2012) Formation of 1D and 2D gold nanoparticle arrays by divalent DNA-gold nanoparticle conjugates. Small 8:2335–2340. https://doi.org/10.1002/smll.201200092
Rangnekar A, Labean TH (2014) Building DNA nanostructures for molecular computation, templated assembly, and biological applications. Acc Chem Res 47:1778–1788. https://doi.org/10.1021/ar500023b
Sato Y, Endo M, Morita M et al (2018) Environment-dependent self-assembly of DNA origami lattices on phase-separated lipid membranes. Adv Mater Interfaces 5:1–9. https://doi.org/10.1002/admi.201800437
Shi X, Lu W, Wang Z et al (2014) Programmable DNA tile self-assembly using a hierarchical sub-tile strategy. Nanotechnology 25:1–10. https://doi.org/10.1088/0957-4484/25/7/075602
Shin J, Kim J, Park SH, Ha TH (2018) Kinetic trans-assembly of DNA nanostructures. ACS Nano 12:9423–9432. https://doi.org/10.1021/acsnano.8b04639
Sung HP, Finkelstein G, LaBean TH (2008) Stepwise self-assembly of DNA tile lattices using dsDNA bridges. J Am Chem Soc 130:40–41. https://doi.org/10.1021/ja078122f
Tagawa M, Shohda KI, Fujimoto K, et al (2007) Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2′-deoxyuridine. Nucleic Acids Res 35:. https://doi.org/10.1093/nar/gkm872
Valdés A, Martínez-García B, Segura J et al (2019) Quantitative disclosure of DNA knot chirality by high-resolution 2D-gel electrophoresis. Nucleic Acids Res 47:e29. https://doi.org/10.1093/nar/gkz015
Wang P, Gaitanaros S, Lee S et al (2016) Programming self-assembly of DNA origami honeycomb two-dimensional lattices and plasmonic metamaterials. J Am Chem Soc 138:7733–7740. https://doi.org/10.1021/jacs.6b03966
Winfree E, Liu F, Wenzler LA, Seeman NC (1998) Design and self-assembly of two-dimensional DNA crystals. Nature 394:539–544. https://doi.org/10.1038/28998
Zhang C, Shao PG, Van Kan JA, Van Der Maarel JRC (2009) Macromolecular crowding induced elongation and compaction of single DNA molecules confined in a nanochannel. Proc Natl Acad Sci U S A 106:16651–16656. https://doi.org/10.1073/pnas.0904741106
Zhang B, Qin X, Zhou M et al (2021) Tetrahedral DNA nanostructure improves transport efficiency and anti-fungal effect of histatin 5 against Candida albicans. Cell Prolif 54:1–9. https://doi.org/10.1111/cpr.13020
Zhao Z, Liu Y, Yan H (2011) Organizing DNA origami tiles into larger structures using preformed scaffold frames. Nano Lett 11:2997–3002. https://doi.org/10.1021/nl201603a
Acknowledgements
The work was supported by Hong Kong Research Grant Council (RGC) funding projects (GRF#16308818, GRF#16309920, and GRF#16309421), Hong Kong Innovation and Technology Commission (HKITC) funding project (MHP/003/19) and the Social Development Project of Guizhou Department of Science and Technology ([No.2020]4Y214).
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Baig, M.M.F.A., Gao, X., Farid, A. et al. Synthesis of stable 2D micro-assemblies of DNA tiles achieved via intrinsic curvatures in the skeleton of DNA duplexes coupled with the flexible support of the twisted side-arms. Appl Nanosci 13, 4279–4289 (2023). https://doi.org/10.1007/s13204-022-02616-1
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DOI: https://doi.org/10.1007/s13204-022-02616-1