Abstract
High permeable drug delivery mechanism is indispensable for the treatment of various diseases including cancer. Protein-polymeric carriers have enhanced the permeability and therapeutic of bioactive compounds. Here, polymethyl methacrylate (PMMA) as polymer and egg white protein of ovalbumin (OVA) from a natural source of quail egg was developed for the highly permeable biocompatible drug delivery system. A significant anti-cancer drug doxorubicin (DOX) was loaded on this drug delivery system. Graphene oxide (GO)-functionalized OVA–PMMA drug delivery system has increased the surface for an accumulation of drug. The drug-loading capacity and controlled release of the drug were investigated through the dialysis technique with various physiological pH environments. The effect of DOX and GO on the morphology of OVA–PMMA matrix was studied with the help of FT-IR and XRD patterns. Dynamic light scattering study gives the data about the particle size of this OVA–PMMA–GO and OVA–PMMA–GO–DOX. These data matched with the image obtained from SEM and TEM instruments. Cytotoxicity effect and cellular uptaking of DOX-loaded OVA–PMMA and OVA–PMMA–GO were investigated on gastric cancer cell line and normal cell line. All these characterizations of this study reveal that the drug is successfully loaded on this new drug carrier and controlled release was achieved.
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Acknowledgements
M. Rajan acknowledges major financial support from the Department of Science and Technology, Science and Engineering Research Board (Ref. YSS/2015/001532; New Delhi, India) and also acknowledges the DST-PURSE program for the purchase of SEM and FT-IR, and UPE programs for the purchase of TEM.
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Prabakaran, S., Jeyaraj, M., Nagaraj, A. et al. Polymethyl methacrylate–ovalbumin @ graphene oxide drug carrier system for high anti-proliferative cancer drug delivery. Appl Nanosci 9, 1487–1500 (2019). https://doi.org/10.1007/s13204-019-00950-5
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DOI: https://doi.org/10.1007/s13204-019-00950-5