Abstract
There is no detailed study on the effects of different ozone doses, in comparison with fungicide, on the quality losses of pomegranates during cold storage. In the present study, the use of ozone as an alternative to fungicide during cold storage of pomegranate was investigated. Pomegranates were exposed to 1, 2 and 3 ppm gaseous ozone for 3 h and dipped into Prochloraz solution (0.9%) for 10 s. Control samples were only treated with air at the same condition. The fruit were packaged in modified atmosphere bags and stored at 6 ºC and 90 ± 5% relative humidity for 4 months. All ozone doses and fungicide treatment suppressed respiration rate and retarded acidity loss in pomegranates. Fungicide and 1 ppm ozone treatments maintained vivid red color of pomegranates during storage. The 1 ppm ozone was the best treatment for decreasing decay rate of fruit, while high doses had a negative effect. Ozone exposure (1 ppm for 3 h) in air can be an alternative to fungicide during cold storage of pomegranate.
Similar content being viewed by others
Abbreviations
- C*:
-
Chroma
- cm:
-
Centimeter
- CO2 :
-
Carbon dioxide
- EU:
-
European Union
- g:
-
Gram
- GC:
-
Gas chromatography
- h:
-
Hour
- hº:
-
Hue angle
- kg:
-
Kilogram
- L*:
-
Skin brightness
- LDPE:
-
Low-density polyethylene
- MAP:
-
Modified atmosphere package
- ml:
-
Milliliter
- O2 :
-
Oxygen
- ppm:
-
Parts per million
- RH:
-
Relative humidity
- s:
-
Second
- SSC:
-
Soluble solids content
- TA:
-
Titratable acidity
- USA:
-
United States of America
- US-FDA:
-
United States—Food and Drug Administration
References
Alexopoulos A, Plessas S, Ceciu S, Lazar V, Mantzourani I, Voidarou C, Stavropoulou E, Bezirtzoglou E (2013) Evaluation of ozone efficacy on the reduction of microbial population of fresh-cut lettuce (Lactuca sativa) and green bell pepper (Capsicum annuum). Food Cont 30:491–496. https://doi.org/10.1016/j.foodcont.2012.09.018
Artes-Hernandez F, Aguayo E, Artes F, Tomas-Barberan FA (2007) Enriched ozone atmosphere enhances bioactive phenolics in seedless table grapes after prolonged shelf life. J Sci Food Agric 87(5):824–831. https://doi.org/10.1002/jsfa.2780
Barman K, Asrey R, Pal RK (2011) Putrescine and carnauba wax pretreatments alleviate chilling injury, enhance shelf life and preserve pomegranate fruit quality during cold storage. Sci Hort 130:795–800. https://doi.org/10.1016/j.scienta.2011.09.005
Bayar-Aydınoğlu D, Koyuncu MA, Erbaş D (2017) Cold storage of pomegranate arils treated with ozone gas. Fruit Sci 4(2):26–32
Bolel H, Koyuncu MA, Erbaş D (2019a) The combined effect of controlled atmosphere with ozone and Prochloraz treatment on storage life and quality of pomegranate cv. Hicaznar Acad J of Agri 8(2):195–202. https://doi.org/10.29278/azd.555195
Bolel H, Koyuncu MA, Erbaş D (2019b) The effects of ozone and fungicide treatments on the fruit quality changes of pomegranate during cold storage. JIST 9(4):1841–1850. https://doi.org/10.21597/jist.551675
Buluc O, Koyuncu MA (2020) Effects of intermittent ozone treatment on postharvest quality and storage life of pomegranate. Ozone-Sci Eng. https://doi.org/10.1080/01919512.2020.1816449
Çakır IO, Koyuncu MA, Erbaş D (2014) The effect of ozone treatment on cold storage of Red Globe grapes. Oral presentation at the meeting of VI. Storage and Marketing Symposium in Horticultural Crops, Bursa, Turkey
De Souza LP, Faroni LRDA, Heleno FF, Cecon PR, Gonçalves TDC, Da Silva GJ, Prates LHF (2018) Effects of ozone treatment on postharvest carrot quality. LWT-Food Sci Technol 90:53–60. https://doi.org/10.1016/j.lwt.2017.11.057
Dilmaçünal T, Erbaş D, Koyuncu MA, Onursal CE, Kuleaşan H (2014) Efficacy of some antimicrobial treatments compared to sodium hypochlorite on physical, physiological and microbial quality of fresh-cut melons (Cucumis melo L. var. inodorus). LWT-Food Sci Technol 59(2):1146–1151. https://doi.org/10.1016/j.lwt.2014.07.033
Elyatem SM, Kader AA (1984) Post-harvest physiology and storage behavior of pomegranate fruits. Sci Hort 24:287–298. https://doi.org/10.1016/0304-4238(84)90113-4
Gabler FM, Smilanick JL, Mansour MF, Karaca H (2010) Influence of fumigation with high concentrations of ozone gas on postharvest gray mold and fungicide residues on table grapes. Postharvest Biol Technol 55(2):85–90. https://doi.org/10.1016/j.postharvbio.2009.09.004
Glowacz M, Colgan R, Rees D (2015) The use of ozone to extend the shelf-life and maintain quality of fresh produce. J Sci Food Agric 95(4):662–671. https://doi.org/10.1002/jsfa.6776
Han Q, Gao H, Chen H, Fang X, Wu W (2017) Precooling and ozone treatments affects postharvest quality of black mulberry (Morus nigra) fruits. Food Chem 221:1947–1953. https://doi.org/10.1016/j.foodchem.2016.11.152
Horvitz S, Cantalejo MJ (2014) Application of ozone for the postharvest treatment of fruits and vegetables. Crit Rev Food Sci Nutr 54(3):312–339. https://doi.org/10.1080/10408398.2011.584353
Karaca H, Velioğlu YS (2014) Effects of ozone treatments on microbial quality and some chemical properties of lettuce, spinach, and parsley. Postharvest Biol Technol 88:46–53. https://doi.org/10.1016/j.postharvbio.2013.09.003
Koyuncu MA, Erbaş D, Onursal CE, Seçmen T, Güneyli A, Üzümcü SS (2019) Postharvest treatments of salicylic acid, oxalic acid and putrescine influences bioactive compounds and quality of pomegranate during controlled atmosphere storage. JFST 56(1):350–359. https://doi.org/10.1007/s13197-018-3495-1
Luo A, Bai J, Li R, Fang Y, Li L, Wang D, Zhang L, Liang J, Huang T, Kou L (2019) Effects of ozone treatment on the quality of kiwifruit during postharvest storage affected by Botrytis cinerea and Penicillium expansum. J Phytopathol 167:470–478. https://doi.org/10.1111/jph.12819
Mersinli E, Koyuncu MA, Erbaş D (2021) Quality retention of minimally processed spinach using low-dose ozonated water during storage. Turk J Agric For 45(2):1–9. https://doi.org/10.3906/tar-2004-75
Ozen T, Koyuncu MA, Erbas D (2020) Effect of ozone treatments on the removal of pesticide residues and postharvest quality in green pepper. JFST. https://doi.org/10.1007/s13197-020-04729-3
Papachristodoulou M, Koukounaras A, Siomos AS, Liakou A, Gerasopoulos D (2017) The effects of ozonated water on the microbial counts and the shelf life attributes of fresh-cut spinach. J Food Process Preserv 42:1–9. https://doi.org/10.1111/jfpp.13404
EU Regulation, (2018). Regulation (EU) 2018/848 of the European Parliament and of the Council of 30 May 2018 on organic production and labelling of organic products and repealing Council Regulation, P. 150.
Selçuk N, Erkan M (2015) Changes in phenolic compounds and antioxidant activity of sour-sweet pomegranates cv. ‘Hicaznar’ during long-term storage under modified atmosphere packaging. Postharvest Biol Technol 109:30–39. https://doi.org/10.1016/j.postharvbio.2015.05.018
Smilanick JL, Crisosto C, Mlikota F (1999) Postharvest use of ozone on fresh fruit. Perish Handl Q 99:10–14
Tabakoğlu N, Karaca H (2018) Effects of ozone-enriched storage atmosphere on postharvest quality of black mulberry fruits (Morus nigra L.). LWT-Food Sci Technol 92:276–281. https://doi.org/10.1016/j.lwt.2018.02.044
USDA: U.S. Department of Agriculture, (2020) Agricultural Marketting Service [accessed 2020 Sep. 22]. http://www.ams.usda.gov/rules-regulations/organic.
Varasteh F, Arzani K, Barzegar M, Zamani Z (2012) Changes in anthocyanins in arils of chitosan-coated pomegranate (Punica granatum L. cv. Rabbab-e-Neyriz) fruit during cold storage. Food Chem 130:267–272. https://doi.org/10.1016/j.foodchem.2011.07.031
Whangchai K, Saengnil K, Uthaibutra K (2006) Effect of ozone in combination with some organic acids on the control of postharvest decay and pericarp browning of longan fruit. Crop Prot 25(8):821–825. https://doi.org/10.1016/j.cropro.2005.11.003
Zhang L, Lu Z, Yu Z, Gao X (2005) Preservation of fresh-cut celery by treatment of ozonated water. Food Cont 16(3):279–283. https://doi.org/10.1016/j.foodcont.2004.03.007
Acknowledgements
The authors wish to thank Dr. Derya ERBAŞ (Isparta University of Applied Sciences, Faculty of Agriculture) for the statistical analysis of data in this study.
Funding
There is no funding for this study.
Author information
Authors and Affiliations
Contributions
The design of this research was done by Mehmet Ali Koyuncu. M.A. Koyuncu also contributed to the interpretation of the results and writing of the article. The physical and chemical analyses in the present study were performed by Oya Buluc.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Consent for publication
All authors agree to publish this article in Journal of Food Science and Technology.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This article was produced from the master’s thesis of first author (Buluc, O.)
Rights and permissions
About this article
Cite this article
Buluc, O., Koyuncu, M.A. Ozone: a promising alternative to prochloraz for cold storage of pomegranate. J Food Sci Technol 59, 2731–2740 (2022). https://doi.org/10.1007/s13197-021-05295-y
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13197-021-05295-y