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Optimization of food-grade medium for co-production of bioactive substances by Lactobacillus acidophilus LA-5 for explaining pharmabiotic mechanisms of probiotic

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Abstract

This study aimed to optimize the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by Lactobacillus acidophilus LA-5 in dairy food-grade by-product. The factorial design revealed that the significant factors were temperature, time, and yeast extract. Then the response surface methodology was used for optimization. At the optimal conditions the viable cell number, CLA, EPSs, and inhibition activity were 2.62 ± 0.49 × 108 CFU/mL, 51.46 ± 1.50 μg/mL, 348.24 ± 5.61 mg/mL and 12.46 ± 0.80 mm, respectively. FTIR, GC, TLC, and SDS page analysis revealed the functional groups of pharmabiotics. The FTIR, GC, TLC, and SDS page analysis showed that both CLA isomers (c-9, t-11, and t-10, c-12) produced. The FTIR, GC, TLC, and SDS page analysis indicated that produced EPSs were composed of glucose, mannose, galactose, xylose, and fructose. FTIR, GC, TLC, and SDS page used to report BACs molecular weight, which showed two fractions by molecular mass 35 and 63 kDa. Previously the ability of different probiotic bacteria investigated and optimized the production of CLA, EPSs, and BACs, but, there was no report on the co-producing capacity of these bioactive metabolites by probiotics. The present work was investigated to optimize the co-production of pharmabiotic metabolites by L. acidophilus LA-5, in supplemented cheese whey as a cultivation medium.

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Authors and Affiliations

  1. Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia, Iran

    Saber Amiri, Mahmoud Rezazadeh Bari & Mohammad Alizadeh

  2. Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

    Saber Amiri, Reza Rezaei Mokarram & Mahmoud Sowti Khiabani

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  1. Saber Amiri
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  2. Reza Rezaei Mokarram
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  3. Mahmoud Sowti Khiabani
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  4. Mahmoud Rezazadeh Bari
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  5. Mohammad Alizadeh
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Correspondence to Saber Amiri or Reza Rezaei Mokarram.

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Amiri, S., Mokarram, R.R., Khiabani, M.S. et al. Optimization of food-grade medium for co-production of bioactive substances by Lactobacillus acidophilus LA-5 for explaining pharmabiotic mechanisms of probiotic. J Food Sci Technol 58, 1–12 (2021). https://doi.org/10.1007/s13197-020-04894-5

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