Abstract
Apium graveolens L. (Apiaceae) is a dietary herb used as a spice, condiment and medicine. A. graveolens (Celery) has been studied for its antimicrobial property and for its application as flavours in food industry. The present study investigated the Apium graveolens oleoresin as an anti-quorum sensing and antibiofilm agent. The quorum sensing and biofilm inhibition study was carried out using biosensor strains Chromobacterium violaceum CV12472 and Pseudomonas aeruginosa PAO1. The MIC of celery oleoresin against C. violaceum CV12472 and P. aeruginosa PAO1 was 10 and 25% v/v, respectively. Inhibition of violacein and biofilm formation was tested at concentrations of oleoresins ranging from 1.56 and 50% v/v. The oleoresins showed a concentration dependent QS inhibitory activity and at sub-MIC of 6.25 and 12.5% v/v, the oleoresins significantly inhibited violacein production and biofilm formation (p < 0.05). Similarly, the celery oleoresin had significant QS modulatory effect on swimming, swarming and twitching motility in P. aeruginosa PAO1 at 12.5% v/v (p < 0.05). The major phytoconstituents present in celery oleoresin as analysed by GC–MS were eicosadiene, benzenemethanol and methyl ester which have not been previously reported. The findings suggest that celery has QS and biofilm inhibitory potential against gram negative pathogens and can find application as food intervention techniques.
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Acknowledgements
This work was funded by DRDO, New Delhi India (Project No. ERIP/ER/201611002/M/01/1672 dated 22nd June 2017). The authors would also like to acknowledge DST-PURSE Program, University of Mysore for cell line facility and IOE, University of Mysore, Mysore, India for fluorescence microscopy facility.
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Nagar, N., Aswathanarayan, J.B. & Vittal, R.R. Anti-quorum sensing and biofilm inhibitory activity of Apium graveolens L. oleoresin. J Food Sci Technol 57, 2414–2422 (2020). https://doi.org/10.1007/s13197-020-04275-y
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DOI: https://doi.org/10.1007/s13197-020-04275-y