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In vitro assessment of Enterococcus faecalis MTC 1032 as the potential probiotic in food supplements

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Abstract

Probiotics become important bacteria in our daily life due to their benefit on human health. In this study, a subset of bacterial strains from children was isolated and evaluated for beneficial probiotic traits such as antimicrobial activity, bile and acid tolerance, and pathogenic cell adherence inhibition. The strain with the best antimicrobial activity was selected for further characterization on the basis of morphological, biochemical characteristics and gene sequence. This strain was Gram-positive, oxidase and catalase-negative, and it produced acids by fermenting sugar and starch as carbon sources. Additionally, it could only hydrolyze bile-esculin, but not red blood cells. The 16S rDNA gene sequence revealed that this strain was Enterococcus faecalis. Interestingly, this strain effectively inhibited a variety of pathogens by acid and bacteriocin production and was bile-tolerant, able to survive under acidic condition. In the safety assessments, E. faecalis MTC 1032 could adhere to host epithelial cells and evidently inhibited pathogenic cell adhesion as demonstrated by cell reduction over time of E. coli ATCC 25922 and S. typhimurium ATCC 13311 on Caco-2 cell line. In summary, it was clearly represented that E. faecalis MTC 1032 provided suitable properties and could be a candidate as a probiotic strain in food supplements.

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Acknowledgements

I would like to thank the Office of the Higher Education Commission, Thailand for supporting the fund under the program of Scholarships for Frontier Research Network for the join Ph.D. Program and Thailand Toray Science Foundation. We would like to extend our thanks to the Thailand Research Fund and Faculty of Pharmacy, Mahidol University (IRG5780007) for financial support on publication. I also thank staffs of the Department of Microbiology, Faculty of Pharmacy, Mahidol University for clarifying several points in this work.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, 10800, Thailand

    Sirikhwan Tinrat

  2. Department of Microbiology, Faculty of Pharmacy, Mahidol University, 447 Sri Ayudthaya Road, Rachathevi, Bangkok, 10400, Thailand

    Piyatip Khuntayaporn, Krit Thirapanmethee & Mullika Traidej Chomnawang

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  1. Sirikhwan Tinrat
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  2. Piyatip Khuntayaporn
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Correspondence to Mullika Traidej Chomnawang.

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Tinrat, S., Khuntayaporn, P., Thirapanmethee, K. et al. In vitro assessment of Enterococcus faecalis MTC 1032 as the potential probiotic in food supplements. J Food Sci Technol 55, 2384–2394 (2018). https://doi.org/10.1007/s13197-018-3155-5

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  • DOI: https://doi.org/10.1007/s13197-018-3155-5

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