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Use of urea-polyacrylamide electrophoresis for discrimination of A1 and A2 beta casein variants in raw cow’s milk

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Abstract

Beta-casein (BC) in cow’s milk occurs in several genetic variants, where BC A1 (BCA1) and BC A2 (BCA2) are the most frequent. This work deals with a method based on modified polyacrylamide gel electrophoresis using urea PAGE to discriminate BCA1 and BCA2 variants from Holstein Friesian (HF) and genetically selected Jersey A2/A2 (JA2) cow’s milk. Two well defined bands were obtained from BC fraction of HF milk, while that of JA2 showed a single band. Proteins from these bands were sequenced by HPLC-quadrupole linear ion trap/mass spectrometry, resulting in BCA1 and BCA2 separation from the BC fraction of HF milk, whereas BCA2 was the only constituent of JA2 fraction. This method represents a feasible and useful tool to on site phenotyping of BC fraction of cow’s milk for pharmaceutical and food industries applications.

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Acknowledgements

Tanks are given to CONACyT-PEI, for Grant No. C003V-2013-01-199586. Authors acknowledge Ecológico Tierra Viva breeders for genotyping control data and provision of milk samples. Authors thank Dr. C. Ferreira-Batista and E. Meneses-Romero from IBT, UNAM, México for technical support in BC sequencing.

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Authors and Affiliations

  1. Centro de Investigación y Desarrollo Tecnológico en Enfermedades Crónicas A.C., Avenida Jurica 122, Parque Industrial Querétaro, 76220, Querétaro, Qro., Mexico

    Miguel Angel Duarte-Vázquez, Carlos Raúl García-Ugalde & Laura Mariana Villegas

  2. DIPA, Facultad de Química, Universidad Autónoma de Querétaro. C.U., Cerro de las Campanas s/n, Col. Las Campanas, 76010, Querétaro, Qro., Mexico

    Blanca Erika Álvarez, Blanca Estela García-Almendárez & Carlos Regalado

  3. Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro Campus Juriquilla, Avenida de las Ciencias s/n, Juriquilla, 76230, Santa Rosa Jáuregui, Qro., Mexico

    Jorge Luis Rosado

Authors
  1. Miguel Angel Duarte-Vázquez
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  2. Carlos Raúl García-Ugalde
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  3. Blanca Erika Álvarez
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  4. Laura Mariana Villegas
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  5. Blanca Estela García-Almendárez
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  6. Jorge Luis Rosado
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  7. Carlos Regalado
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Correspondence to Carlos Regalado.

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Duarte-Vázquez, M.A., García-Ugalde, C.R., Álvarez, B.E. et al. Use of urea-polyacrylamide electrophoresis for discrimination of A1 and A2 beta casein variants in raw cow’s milk. J Food Sci Technol 55, 1942–1947 (2018). https://doi.org/10.1007/s13197-018-3088-z

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  • DOI: https://doi.org/10.1007/s13197-018-3088-z

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