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Effect of the essential oils from Melaleuca alternifolia, Melaleuca quinquenervia and Backhousia citriodora on the synthesis of ochratoxin A by Aspergillus niger and Aspergillus carbonarius isolated from tropical wine grapes

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Abstract

The influence of essential oils (EOs) extracted from the leaves of Melaleuca alternifolia, Melaleuca quinquenervia and Backhousia citriodora on ochratoxin A (OTA) synthesis by fungi was studied. The extraction of EOs was performed by hydrodistillation (Clevenger apparatus) over a 2-h period and subsequently analyzed by GC–MS and GC–FID. The toxigenic activity of the essential oils (31.25; 15.62 and 7.81 µg mL−1) was evaluated by inhibiting the production of OTA by Aspergillus niger and Aspergillus carbonarius in Czapek agar medium culture. The quantification of the toxin was performed by HPLC. The production of OTA was dependent on the fungal species, incubation temperature (15 and 25 °C) and the presence of the essential oils. In tests carried out at 15 °C, the oils caused a reduction in OTA synthesis that ranged from 57.60 to 76.93% and from 54.78 to 98.68% for the fungal species A. carbonarius and A. niger, respectively. At 25 °C, reductions ranged from the 38.66 to 75.93% and from 17.94 to 71.79% for the respective fungi. The study concluded that natural products could be potential biocontrol agents against OTA contamination in food.

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Acknowledgements

The authors thanks the support of the Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).

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Authors and Affiliations

  1. Department of Chemistry, Federal University of Lavras, Lavras, MG, CEP: 37200-000, Brazil

    Juliana de Andrade Santiago, Maria das Graças Cardoso & Wilder Douglas Santiago

  2. Department of Food Science, Federal University of Lavras, Lavras, MG, CEP: 37200-000, Brazil

    Luis Roberto Batista, Fabiana Reinis Franca Passamani & Leonardo Milani Avelar Rodrigues

  3. Federal University of Vales de Jequitinhonha e Mucuri, Diamantina, MG, CEP: 39100-000, Brazil

    David Lee Nelson

Authors
  1. Juliana de Andrade Santiago
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  2. Maria das Graças Cardoso
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  3. Luis Roberto Batista
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  4. Wilder Douglas Santiago
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  5. Fabiana Reinis Franca Passamani
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  6. Leonardo Milani Avelar Rodrigues
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  7. David Lee Nelson
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Correspondence to Maria das Graças Cardoso.

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de Andrade Santiago, J., Cardoso, M., Batista, L.R. et al. Effect of the essential oils from Melaleuca alternifolia, Melaleuca quinquenervia and Backhousia citriodora on the synthesis of ochratoxin A by Aspergillus niger and Aspergillus carbonarius isolated from tropical wine grapes. J Food Sci Technol 55, 418–423 (2018). https://doi.org/10.1007/s13197-017-2857-4

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  • DOI: https://doi.org/10.1007/s13197-017-2857-4

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