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Isolation and characterization of lactic acid bacteria from traditional pickles of Himachal Pradesh, India

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Abstract

A total of 15 isolates of lactic acid bacteria (LAB) were isolated from traditional pickles of rural and urban areas of Himachal Pradesh. These isolates were identified as Enterococcus faecalis (7), Lactobacillus plantarum (3), Pediococcus pentosaceus (2), Leuconostoc mesenteroides (1), Lactococcus lactis (1) and Enterococcus sp. (1) on the basis of morphological, biochemical and 16S rDNA sequence analysis. All the LABs produced exopolysaccharides, have antibiotic susceptibility, and possess activity of β-galactosidase, β-glucosidase, protease and amylase, while none of the isolates showed haemolytic activity. Out of 15 isolates tested, only 6 isolates showed the phytase activity. These LAB exhibited antimicrobial activity against food borne pathogenic bacteria i.e. Bacillus cereus, Escherichia coli, Staphylococcus aureus and Shigella dysenteriae. These results suggested that LAB isolates from traditional pickles of Himachal Pradesh have very good potential to be used as probiotics.

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Acknowledgements

The financial support in the form of Maulana Azad National Fellowship and Post Doctoral Fellowship by University Grants Commission (UGC) (Grant Nos. F1-17.1/2010/MANF-BUD-HIM-3917/(SA-III/website), F.15-13/12(SA-II)) New Delhi is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171 005, India

    Monika,  Savitri, Vijay Kumar, Anila Kumari, Kunzes Angmo & Tek Chand Bhalla

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  1. Monika
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  2. Savitri
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  3. Vijay Kumar
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  4. Anila Kumari
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  5. Kunzes Angmo
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  6. Tek Chand Bhalla
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Correspondence to Tek Chand Bhalla.

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Monika, Savitri, Kumar, V. et al. Isolation and characterization of lactic acid bacteria from traditional pickles of Himachal Pradesh, India. J Food Sci Technol 54, 1945–1952 (2017). https://doi.org/10.1007/s13197-017-2629-1

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  • DOI: https://doi.org/10.1007/s13197-017-2629-1

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