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Antibiotic susceptibility and heterogeneity in technological traits of lactobacilli isolated from Algerian goat’s milk

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Abstract

The objective of this study was to identify and study the heterogeneity of technological traits of lactobacilli from goat’s milk of Algeria and to evaluate in vitro their safety aspect. Using API50 CHL system and 16S rDNA sequencing, 51 % of strains were assigned as Lactobacillus plantarum, 34 % as L. pentosus, 7 % as L. rhamnosus and 8 % as L. fermentum. A large variability was noted for the acidifying capacity in skim milk after 6, 12 and 24 h of incubation. All strains expressed aminopeptidase activity against alanine-ρ-NA and leucine-ρ-NA at different levels. All strains were resistant to vancomycin and most of strains showed more susceptibility to β-lactam antibiotic. High susceptibility toward the inhibitors of protein synthesis was also observed. Minimum inhibitory concentrations data obtained revealed that isolates were susceptible to penicillin and chloramphenicol, and resistant to gentamicin and vancomycin. Minimum inhibitory concentrations distribution of other antibiotics showed variability. The analysis of graphical representation of principal component analysis of technological properties of L. plantarum and L. pentosus strains showed diversity among the isolates. Finally, eight L. plantarum (LAM1, LAM3, LAM21, LAM25, LAM35, LF15, LAM34, and LAM35), four L. pentosus (LAM38, LAM39, LF9 and LF16) and two L. rhamnosus (LF3 and LF10) strains, could be good candidates as adjunct culture in dairy product in Algeria.

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Acknowledgments

Authors would like to expresses their gratitude to “Ministère de l’Enseignement Supérieur et de la Recherche Scientifique Algérien” and the Spanish Ministry of Science and Innovation for partially funded of this work. We are grateful to Dr Gaspar Perez Martinez (Director of laboratory of lactic acid bacteria and probiotic) - Instituto de Agroquímica y Tecnología de los Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Burjassot -Valencia- Espagne, for our acceptance in the laboratory and Dr Manuel Zúñiga for him excellent technical assistance and helpful.

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  1. Department of Biology, Faculty of Natural and Life Sciences, University Djillali Liabes, Sidi Bel Abbès, 22000, Algeria

    Leila Bousmaha-Marroki & Ahmed Marroki

  2. Laboratory of Microbial Genetic - Faculty of Natural and Life Sciences, University of Oran, Oran, 31100, Algeria

    Ahmed Marroki

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  1. Leila Bousmaha-Marroki
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Correspondence to Ahmed Marroki.

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Bousmaha-Marroki, L., Marroki, A. Antibiotic susceptibility and heterogeneity in technological traits of lactobacilli isolated from Algerian goat’s milk. J Food Sci Technol 52, 4708–4723 (2015). https://doi.org/10.1007/s13197-014-1556-7

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