Abstract
Elsinoë ampelina, the causal agent of grapevine anthracnose, infects all aerial parts of the plant, causing severe grapes production losses. Essential oils, as natural antifungal product, are amongst the most promising natural plant protection alternatives because they are recognized as safe substances and show high antifungal activity against phytopathogenic fungi. The present study aimed to determine Eucalyptus staigeriana essential oil effect in vitro and in vivo against E. ampelina. The essential oil exhibited antifungal activity inhibiting mycelial growth and conidial germination at 0.15 and 1 µL mL−1 concentrations, respectively. Moreover, essential oil application in vivo reduced the disease incidence by 40% and severity by more than 60% in preventive treatment, and in curative treatment, the incidence was reduced by exceeding 30% and severity by more than 80%. The present results indicated that E. staigeriana essential oil could be a natural fungicidal product for controlling anthracnose caused by E. ampelina in grapevines.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adams RP (2005) Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Carol Stream, p 46
Ammad F, Moumen O, Gasem A, Othmane S, Hisashi K-N, Zebib B, Merah O (2018) The potency of lemon (Citrus limon L.) essential oil to control some fungal diseases of grapevine wood. Comptes Rendus Biologies 341(2):97–101. https://doi.org/10.1016/j.crvi.2018.01.003
Brooker MIH, Keing DA (2004) Field guide to Eucalyptus, 2nd edn. Bloomings Book, Melbourne
Burt S (2004) Essential oils: their antibacterial properties and potential applications in foods — a review. Int J Food Microbiol 94:23–253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022
Capote N, Pastrana AM, Aguado A, Sánchez-Torres P (2012) Molecular tools for detection of plant pathogenic fungi and fungicide resistance. Plant pathology 151–202.
Carisse O, Lefebvre A (2011) A model to estimate the amount of primary inoculum of Elsinoë ampelina. Plant Dis 95:1167–1171. https://doi.org/10.1094/PDIS-11-10-0798
Carisse O, Morissette-Thomas V (2013) Epidemiology of grape anthracnose: factors associated with defoliation of grape leaves infected by Elsinoë ampelina. Plant Dis 97:222–230. https://doi.org/10.1094/PDIS-04-12-0393-RE
Carisse O, Levasseur A, Provost C (2020) Influence of leaf wetness duration and temperature on infection of grape leaves by Elsinoe ampelina under controlled and vineyard conditions. Plant Dis 104:2817–2822
Carisse O, Levasseur A, Provost C (2021) Anthracnose risk establishment based on age-related susceptibility of grape leaves, flowers and berries to infection by Elsinoe ampelina. Plant Disease.https://doi.org/10.1094/PDIS-09-20-2060-RE
Fialho RO, Papa MFS (2015) Atividade antifúngica in vitro de óleos essenciais sobre Sphaceloma ampelinum. Cultura Agronômica 24:63–70
Isman MB (2000) Plant essential oils for pest and disease management. Crop Prot 19:603–608. https://doi.org/10.1016/S0261-2194(00)00079-X
La Torre A, Mandalà C, Pezza L, Caradonia F, Battaglia V (2014) Evaluation of essential plant oils for the control of Plasmopara viticola. J Essent Oil Res 26(4):282–291. https://doi.org/10.1080/10412905.2014.889049
Li Z, dos Santos RF, Gao L, Chang P, Wang X (2021) Current status and future prospects of grapevine anthracnose caused by Elsinoe ampelina: an important disease in humid grape-growing regions. Mol Plant Pathol 22:899–910. https://doi.org/10.1111/mpp.13076
Macedo ITF, Bevilaqua CML, Oliveira LMB, Camurça-Vasconcelos ALF, Vieira LS, Oliveira FR, Queiroz-Junior EM, Tomé AR, Nascimento NRF (2010) Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Vet Parasitol 173:93–98. https://doi.org/10.1016/j.vetpar.2010.06.004
Magarey RD, Emmett RW, Magarey PA, Franz PR (1993) Evaluation of control of grapevine anthracnose caused by Elsinoë ampelina by pre-infection fungicides. Australas Plant Pathol 22:48–52. https://doi.org/10.1071/APP9930048
Marzoug HNB, Romdhane M, Lebrihi A, Mathieu F, Couderc F, Abderraba M, Khouja ML, Bouajila J (2011) Eucalyptus oleosa essential oils: chemical composition and antimicrobial and antioxidant activities of the oils from different plant parts (stems, leaves, flowers and fruits). Molecules 16(2):1695–2170. https://doi.org/10.3390/molecules16021695
Negi PS (2012) Plant extracts for the control of bacterial growth: efficacy, stability and safety issues for food application. Int J Food Microbiol 156:7–17. https://doi.org/10.1016/j.ijfoodmicro.2012.03.006
Oliveira DH, Abib PB, Giacomini RX, Lenardão EJ, Schiedeck G, Ethel EA, Luchese C, Savegnago L, Jacob RG (2019) Antioxidant and antifungal activities of the flowers’ essential oil of Tagetes minuta, (Z)-tagetone and thiotagetone. J Essent Oil Res 31(2):160–169. https://doi.org/10.1080/10412905.2018.1519465
Pedrotti C, Marcon ÂR, Delamare APL, Echeverrigaray SL, Ribeiro RTDS, Schwambach J (2019) Alternative control of grape rots by essential oils of two Eucalyptus species. J Sci Food Agric 99(14):6552–6561. https://doi.org/10.1002/jsfa.9936
Pedrotti C, Marcon ÂR, Delamare APL, Echeverrigaray SL, Ribeiro RTDS, Schwambach J (2020) Essential oil as sustainable alternative for diseases management of grapes in postharvest and in vineyard and its influence on wine. Journal of Environmental Science and Health Part B 56(1):73–81. https://doi.org/10.1080/03601234.2020.1838827
Petit AN, Wojnarowiez G, Panon ML, Baillieul F, Clément C, Fontaine F, Vaillant-Gaveau N (2009) Botryticides affect grapevine leaf photosynthesis without inducing defense mechanisms. Planta 229:497–506. https://doi.org/10.1007/s00425-008-0849-3
Rozwalka LC, Moreira RR, Garcia MJB, Marques FA, May De Mio LL (2020) Chemical components of essential oils as a base to control two grape pathogens: Sphaceloma ampelinum and Pseudocercopora vitis. J Phytopathol 168:342–352. https://doi.org/10.1111/jph.12898
Santos RF, Ciampi-Guillardi M, Amorim L, Massola Júnior NS, Spósito MB (2018a) Aetiology of anthracnose on grapevine shoots in Brazil. Plant Pathol 67:692–706. https://doi.org/10.1111/ppa.12756
Santos RF, Spósito MB, Ayres MR, Sosnowski MR (2018b) b) Phylogeny, morphology and pathogenicity of Elsinoë ampelina, the causal agent of grapevine anthracnose in Brazil and Australia. J Phytopathol 166:187–198. https://doi.org/10.1111/jph.12675
Santos RF, Spósito MB, Ayres MR, Sosnowski MR (2018c) c) In vitro production of conidia of Elsinoë ampelina, the causal fungus of grapevine anthracnose. Eur J Plant Pathol 152:815–821. https://doi.org/10.1007/s10658-018-1502-z
Scariot FJ, Foresti L, Delamare APL, Echeverrigaray S (2020) Activity of monoterpenoids on the in vitro growth of two Colletotrichum species and the mode of action on C. acutatum. Pesticide Biochemistry and Physiology 170. https://doi.org/10.1016/j.pestbp.2020.104698
Tang X, Shao Y, Tang Y, Zhou W (2018) Antifungal activity of essential oil compounds (geraniol and citral) and inhibitory mechanisms on grain pathogens (Aspergillus flavus and Aspergillus ochraceus). Molecules 23:2108. https://doi.org/10.3390/molecules23092108
Thind SK, Arora JK, Kaur N, Monga PK, Arora PK (2001) Periodicity and prediction model of grape anthracnose in Punjab an agrometeorological approach. Plant Disease Research 16:63–67
Thind TS (2015) Anthracnose. In Compendium of grape diseases, disorders, and pests. 2nd Edn., eds Wilcox WF, Gubler WD, Uyemoto JK (St. Paul: APS Press) 17–19.
Tomazoni EZ, Pauletti GF, Ribeiro RTS, Moura S, Schwambach J (2017) In vitro and in vivo activity of essential oils extracted from Eucalyptus staigeriana, Eucalyptus globulus and Cinnamomum camphora against Alternaria solani Sorauer causing early blight in tomato. Sci Hortic 223:72–77. https://doi.org/10.1016/j.scienta.2017.04.033
Tomazoni EZ, Griggio GS, Broilo EP, Ribeiro RTS, Soares GLG, Schwambach J (2018) Screening for inhibitory activity of essential oils on fungal tomato pathogen Stemphylium solani Weber. Biocatalysis and Agricultural Biotecnology 16:364–372. https://doi.org/10.1016/j.bcab.2018.08.012
Tsakirakis A, Tsatsakis A, Tsakalof A, Kasiotis K, Machera K, Christou A (2012) Operator exposure during fungicide applications in vineyards. Toxicol Lett 211. https://doi.org/10.1016/j.toxlet.2012.03.788
Umereweneza D, Muhizi T, Kamizikunze T, Nkurunziza JP (2019) Chemical composition and antifungal activity of essential oils extracted from leaves of Eucalyptus melliodora and Eucalyptus anceps grown in Rwanda. J Essent Oil Bearing Plants 22(1):151–158. https://doi.org/10.1080/0972060X.2019.1585297
Van Den Dool H, Kratz PDA (1963) Generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography. J Chromatogr A 11:463–471. https://doi.org/10.1016/s0021-9673(01)80947-x
Yoldashkhan A, Samavat S, Zamanizadeh H (2018) In-vitro antifungal activity of several biorational fungicides against Calonectria pseudonaviculata, the causal agent of Buxus hyrcana blight. Forest Pathol 48(5):e12442. https://doi.org/10.1111/efp.12442
Zheng S, Jing G, Wang X, Ouyang Q, Jia L, Tao N (2015) Citral exerts its antifungal activity against Penicillium digitatum by affecting the mitochondrial morphology and function. Food Chem 178:76–81. https://doi.org/10.1016/j.foodchem.2015.01.077
Zhou D, Wang Z, Li M, Xing M, Xian T, Tu K (2017) Carvacrol and eugenol effectively inhibit Rhizopus stolonifer and control postharvest soft rot decay in peaches. J Appl Microbiol 124(1):166–178. https://doi.org/10.1111/jam.13612
Acknowledgements
The authors acknowledge Ricardo Feliciano dos Santos (University of São Paulo) and Lenon Modesto (Federal University of Santa Catarina, Brazil) for providing the fungal strain for the development of this study.
Funding
This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES)—Finance Code 001.
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior,001,Carine Pedrotti
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflicts of interest
The authors declare no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Pedrotti, C., Caro, I.M.D.D., Franzoi, C. et al. Control of anthracnose (Elsinoë ampelina) in grapevines with Eucalyptus staigeriana essential oil. Org. Agr. 12, 81–89 (2022). https://doi.org/10.1007/s13165-021-00382-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13165-021-00382-y