Skip to main content
SpringerLink
Log in

Influence du traitement présomptif intermittent par la sulfadoxinepyriméthamine sur l’acquisition d’anticorps anti-VAR2CSA chez la femme enceinte vivant en zone hypoendémique au Sénégal

Effect of intermittent presumptive treatment with sulfadoxine-pyrimethamine on the acquisition of anti-VAR2CSA antibodies in pregnant women living in a hypoendemic area in Senegal

  • Santé Publique / Public Health
  • Published:
Bulletin de la Société de pathologie exotique

Résumé

L’impact du traitement présomptif intermittent (TPI) sur l’immunité de la femme enceinte au Sénégal n’est pas très bien connu. L’objectif de cette étude était d’évaluer la production des immunoglobulines (IgG) dirigées contre les protéines MSP1, GLURP et DBL5 entre l’inclusion et l’accouchement chez des femmes enceintes sous TPI. Nous avons mené une étude prospective au niveau de la maternité Roi-Baudouin de Guédiawaye (RBG) [Sénégal]. Les femmes incluses dans cette étude ont subi des prélèvements sanguins à l’inclusion et à l’accouchement. Les échantillons de sang collectés ont été analysés après centrifugation pour la détection des IgG, par technique Elisa à partir des sérums. Un consentement libre et éclairé était obtenu préalablement à l’inclusion des femmes. Au total, 101 femmes âgées de 18 à 44 ans étaient incluses dans l’étude. Les femmes multigestes représentaient 70,3 % de la population d’étude, tandis que les primigestes représentaient 29,7 %. Le dosage des IgG a révélé de faibles variations entre l’inclusion et l’accouchement des femmes pour ce qui est des IgG anti-MSP1 (83,1 contre 79,5; p = 0,52), de même que pour les IgG anti-GLURP-R2 (84,1 contre 75,9; p = 0,16). Après ajustement sur la gestité, on note une baisse significative de la production d’anticorps anti-VAR2CSA entre l’inclusion et l’accouchement des femmes de l’étude (p < 0,05). En diminuant l’incidence du paludisme pendant la grossesse, le TPI réduit l’acquisition des anticorps antiparasites placentaires, ce qui retarderait l’installation d’une immunité antipalustre protectrice. L’application du TPI chez la femme enceinte serait de ce fait plus indiquée en zone hypoendémique où l’exposition au paludisme est plus faible.

Abstract

The impact of intermittent presumptive treatment (IPT) on the immunity of pregnant women in Senegal is still not very well known. We conducted a prospective study at the Roi-Baudouin maternity of Guediawaye in Senegal to assess IgG antibodies production against MSP1, GLURP and DBL5 in pregnant women under IPT. Blood samples were collected from the participating women at inclusion and delivery. Samples were analyzed after centrifugation for the detection of IgG antibodies in sera by Elisa. Informed consent was given by each study participant prior to their inclusion. A total of 101 eligible women aged from 18 to 44 were included in this study. Multigravidae women represented 70.3% of the study population, whereas primigravidae accounted for 29.7%. The IgG level decreased slightly from inclusion to delivery for the women with regard to anti-MSP1 (83.1at inclusion versus 79.5 at delivery, p = 0.52) as well as anti-GLURP-R2 (84.1 at inclusion versus 75.9 at delivery, p = 0.16). After adjustment for number of pregnancies, there was a significant decrease in the production of anti-VAR2CSA between inclusion and delivery (p < 0.05). By reducing the incidence of malaria during pregnancy, IPT reduced the acquisition of placental parasites antibodies suppressors which could delay the development of protective immunity against malaria. The application of IPT in pregnant women would thus be more appropriate in hypoendemic areas where malaria exposure is lower.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Références

  1. Asa OO, Onayade AA, Fatusi AO, et al (2008) Efficacy of intermittent preventive treatment of malaria with sulphadoxine-pyrimethamine in preventing anaemia in pregnancy among Nigerian women. Matern Child Health J 12(6):692–698. Epub 2008

    Article  PubMed  CAS  Google Scholar 

  2. Barfod L, Dobrilovic T, Magistrado P, et al (2010) Chondroitin sulfate A-adhering Plasmodium falciparum-infected erythrocytes express functionally important antibody epitopes shared by multiple variants. J Immunol 185(12):7553–7561. Epub 2010

    Article  PubMed  CAS  Google Scholar 

  3. Beeson JG, Rogerson SJ, Cooke BM, et al (2000) Adhesion of Plasmodium falciparum-infected erythrocytes to hyaluronic acid in placental malaria. Nat Med 6(1):86–90

    Article  PubMed  CAS  Google Scholar 

  4. Bouyou-Akotet MK, Ionete-Collard DE, Mabika-Manfoumbi M, et al (2003) Prevalence of Plasmodium falciparum infection in pregnant women in Gabon. Malar J 2:18. Epub 2003

    Article  PubMed  Google Scholar 

  5. Camara B, Diack B, Diouf S, et al (1995) Les faibles poids de naissance: fréquence et facteurs de risque dans le district de Guédiawaye (banlieue de Dakar). Dakar Med 40(2):213–219

    PubMed  CAS  Google Scholar 

  6. Chaouat G, Cayol V, Mairovitz V, Dubanchet S (1999) Localization of the Th2 cytokines IL-3, IL-4, IL-10 at the fetomaternal interface during human and murine pregnancy and lack of requirement for Fas/Fas ligand interaction for a successful allogeneic pregnancy. Am J Reprod Immunol 42(1):1–13

    Article  PubMed  CAS  Google Scholar 

  7. Dicko A, Mantel C, Thera MA, et al (2003) Risk factors for malaria infection and anemia for pregnant women in the Sahel area of Bandiagara, Mali. Acta Trop 89(1):17–23

    Article  PubMed  Google Scholar 

  8. Duffy PE, Fried M (2003) Antibodies that inhibit Plasmodium falciparum adhesion to chondroitin sulfate A are associated with increased birth weight and the gestational age of newborns. Infect Immun 71(11):6620–6623

    Article  PubMed  CAS  Google Scholar 

  9. Fievet N, Moussa M, Tami G, et al (2001) Plasmodium falciparum induces a Th1/Th2 disequilibrium, favoring the Th1-type pathway, in the human placenta. J Infect Dis 183(10):1530–1534. Epub 2001

    Article  PubMed  CAS  Google Scholar 

  10. Fried M, Duffy PE (1996) Adherence of Plasmodium falciparum to chondroitin sulfate A in the human placenta. Science 272(5267): 1502–1504

    Article  PubMed  CAS  Google Scholar 

  11. Fried M, Duffy PE (1998) Maternal malaria and parasite adhesion. J Mol Med 76(3–4):162–171

    Article  PubMed  CAS  Google Scholar 

  12. Fried M, Duffy PE (2002) Two DBLgamma subtypes are commonly expressed by placental isolates of Plasmodium falciparum. Mol Biochem Parasitol 122(2):201–210

    Article  PubMed  CAS  Google Scholar 

  13. Fried M, Muga RO, Misore AO, Duffy PE (1998) Malaria elicits type 1 cytokines in the human placenta: IFN-gamma and TNFalpha associated with pregnancy outcomes. J Immunol 160(5): 2523–2530

    PubMed  CAS  Google Scholar 

  14. Gnidehou S, Jessen L, Gangnard S, et al (2010) Insight into antigenic diversity of VAR2CSA-DBL5ɛ domain from multiple Plasmodium falciparum placental isolates. PLoS One 5(10). pii: e13105

    Article  PubMed  Google Scholar 

  15. Guitard J, Andersen P, Ermont C, et al (2010) Plasmodium falciparum population dynamics in a cohort of pregnant women in Senegal. Malar J 9:165

    Article  PubMed  Google Scholar 

  16. Guitard J, Cottrell G, Magnouha NM, et al (2008) Differential evolution of anti-VAR2CSA-IgG3 in primigravidae and multigravidae pregnant women infected by Plasmodium falciparum. Malar J 7:10

    Article  PubMed  Google Scholar 

  17. Koudou BG, Ghattas H, Essé C, et al (2010) The use of insecticide-treated nets for reducing malaria morbidity among children aged 6–59 months, in an area of high malaria transmission in central Côte-d’Ivoire. Parasit Vectors 3:91

    Article  PubMed  Google Scholar 

  18. Lancastre F, Combrisson A, Le Bras J, et al (1988) Un cas de chloroquinorésistance de Plasmodium falciparum en Côte-d’Ivoire. Presse Med 17(12):589

    PubMed  CAS  Google Scholar 

  19. Marques HH, Vallada MG, Sakane PT, Boulos M (1996) Congenital malaria. Case reports and a brief review of literature (article en portugais, résumé en anglais). J Pediatr (Rio J) 72(2):103–105

    Google Scholar 

  20. Maubert B, Guilbert LJ, Deloron P (1997) Cytoadherence of Plasmodium falciparum to intercellular adhesion molecule 1 and chondroitin-4-sulfate expressed by the syncytiotrophoblast in the human placenta. Infect Immun 65(4):1251–1257

    PubMed  CAS  Google Scholar 

  21. Mbonye AK, Bygbjerg I, Magnussen P (2007) Intermittent preventive treatment of malaria in pregnancy: evaluation of a new delivery approach and the policy implications for malaria control in Uganda. Health Policy 81(2–3):228–241. Epub 2006

    Article  PubMed  Google Scholar 

  22. Menon R (1972) Pregnancy and malaria. Med J Malaya 27(2):115–119

    PubMed  CAS  Google Scholar 

  23. Okoko BJ, Ota MO, Yamuah LK, et al (2002) Influence of placental malaria infection on foetal outcome in the Gambia: twenty years after Ian Mcgregor. J Health Popul Nutr 20(1):4–11

    PubMed  Google Scholar 

  24. Plasse M, Peyron F, Francois P, et al (1990) Aspects cliniques inhabituels de paludisme à Plasmodium falciparum. Pédiatrie 45(5):311–314

    PubMed  CAS  Google Scholar 

  25. Raccurt CP, Le Bras M, Le Bras J, et al (1986) À propos d’un cas fatal de neuropaludisme chloroquinorésistant chez un voyageur au Cameroun. Bull Soc Pathol Exot Filiales 79(1):39–49

    PubMed  CAS  Google Scholar 

  26. Rogerson SJ, Chaiyaroj SC, Ng K, et al (1995) Chondroitin sulfate A is a cell surface receptor for Plasmodium falciparum-infected erythrocytes. J Exp Med 182(1):15–20

    Article  PubMed  CAS  Google Scholar 

  27. Salanti A, Dahlbäck M, Turner L, et al (2004) Evidence for the involvement of VAR2CSA in pregnancy-associated malaria. J \Exp Med 200(9):1197–1203

    Article  PubMed  CAS  Google Scholar 

  28. Scherf A, Pouvelle B, Buffet PA, Gysin J (2001) Molecular mechanisms of Plasmodium falciparum placental adhesion. Cell Microbiol 3(3):125–131

    Article  PubMed  CAS  Google Scholar 

  29. Staalsoe T, Megnekou R, Fievét N, et al (2001) Acquisition and decay of antibodies to pregnancy-associated variant antigens on the surface of Plasmodium falciparum-infected erythrocytes that protect against placental parasitemia. J Infect Dis 184(5):618–626. Epub 2001

    Article  PubMed  CAS  Google Scholar 

  30. Staalsoe T, Shulman CE, Bulmer JN, et al (2004) Variant surface antigen-specific IgG and protection against clinical consequences of pregnancy-associated Plasmodium falciparum malaria. Lancet 363(9405):283–289

    Article  PubMed  CAS  Google Scholar 

  31. Staalsoe T, Shulman CE, Dorman EK, et al (2004) Intermittent preventive sulfadoxine-pyrimethamine treatment of primigravidae reduces levels of plasma immunoglobulin G, which protects against pregnancy-associated Plasmodium falciparum malaria. Infect Immun 72(9):5027–5030

    Article  PubMed  CAS  Google Scholar 

  32. Steketee RW, Breman JG, Paluku KM, et al (1988) Malaria infection in pregnant women in Zaire: the effects and the potential for intervention. Ann Trop Med Parasitol 82(2):113–120

    PubMed  CAS  Google Scholar 

  33. Trape JF, Lefebvre-Zante E, Legros F, et al (1992) Vector density gradients and the epidemiology of urban malaria in Dakar, Senegal. Am J Trop Med Hyg 47(2):181–189

    PubMed  CAS  Google Scholar 

  34. Tuikue Ndam NG, Salanti A, Le-Hesran JY, et al (2006) Dynamics of anti-VAR2CSA immunoglobulin G response in a cohort of Senegalese pregnant women. J Infect Dis 193(5):713–720. Epub 2006

    Article  PubMed  CAS  Google Scholar 

  35. Viebig NK, Gamain B, Scheidig C, et al (2005) A single member of the Plasmodium falciparum var multigene family determines cytoadhesion to the placental receptor chondroitin sulphate A. EMBO Rep 6(8):775–781

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Service de parasitologie médicale, faculté de médecine de l’université Cheikh-Anta-Diop de Dakar, BP 5005, Dakar Fann, Sénégal

    I. Diouf, R. C. K. Tine, J. L. Ndiaye, K. Sylla, B. Faye, M. L. Mengue, O. Faye, Y. Dieng & O. Gaye

  2. District sanitaire de Guédiawaye, centre de santé Roi-Baudouin de Guédiawaye, ministère de la Santé et de la Prévention, Dakar, Sénégal

    A. Gaye

Authors
  1. I. Diouf
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. K. Tine
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. L. Ndiaye
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Sylla
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. B. Faye
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. L. Mengue
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. O. Faye
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Y. Dieng
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. A. Gaye
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. O. Gaye
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to R. C. K. Tine.

About this article

Cite this article

Diouf, I., Tine, R.C.K., Ndiaye, J.L. et al. Influence du traitement présomptif intermittent par la sulfadoxinepyriméthamine sur l’acquisition d’anticorps anti-VAR2CSA chez la femme enceinte vivant en zone hypoendémique au Sénégal. Bull. Soc. Pathol. Exot. 104, 277–283 (2011). https://doi.org/10.1007/s13149-011-0153-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13149-011-0153-5

Mots clés

Keywords

Search

Navigation